“…The lipid bilayer is neither mechanically nor electrically stable [31]. Several approaches have been conducted to overcome this inherent limitation such as the inclusion of polymerizable lipids [32,33], the use of hydrogels and inorganic supports [34,35], reduction of the lateral bilayer size [36], 'droplet interface bilayers' (DIBs) [37,38], and replacement of the lipids by amphiphilic polymers [26]. The protein itself is not very stable and has a relatively short lifetime for detection as a result of the sensitivity of the protein to temperature, voltage, ion concentrations, and solvents [39,40].…”