Highly Multiplexed, Efficient, and Automated Single‐Cell MicroRNA Sequencing with Digital Microfluidics.
Yingwen Chen,
Xuanqun Wang,
Xing Na
et al.
Abstract:Single‐cell microRNA (miRNA) sequencing has allowed for comprehensively studying the abundance and complex networks of miRNAs, which provides insights beyond single‐cell heterogeneity into the dynamic regulation of cellular events. Current benchtop‐based technologies for single‐cell miRNA sequencing are low throughput, limited reaction efficiency, tedious manual operations, and high reagent costs. Here, a highly multiplexed, efficient, integrated, and automated sample preparation platform is introduced for sin… Show more