Highly fluorescent semiconductor core–shell CdTe–CdS nanocrystals for monitoring living yeast cells activity

Farias, Patrícia M. A.; Santos, Beate S.; Menezes, Frederico Duarte de; Brasil, A.G.; Ferreira, Ricardo; Motta, Mauricio; Neto, Antônio Gomes de Castro; Vieira, A.A.S.; Silva, D.C.N.; Fontes, Adriana; César, Carlos L.

doi:10.1007/s00339-007-4267-3

Cited by 28 publications

(14 citation statements)

References 15 publications

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“…Herein, we demonstrate that QDs prepared in our own facilities were successfully used for imaging of living parasites and interaction studies. Others studies with living cells as macrophages , yeast cells (Farias et al 2007) and also quantum dots as fluorescent biolabel in cancer diagnostic (Farias et al 2006), showed the low level of cytotoxity of this material in comparison with others fluorophores.…”

Section: Discussionmentioning

confidence: 97%

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

et al. 2009

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Semiconductor quantum dots (QDs) are highly fluorescent nanocrystals markers that allow long photobleaching and do not destroy the parasites. In this paper, we used fluorescent core shell quantum dots to perform studies of live parasite-vector interaction processes without any observable effect on the vitality of parasites. These nanocrystals were synthesized in aqueous medium and physiological pH, which is very important for monitoring live cells activities, and conjugated with molecules such as lectins to label specific carbohydrates involved on the parasite-vector interaction. These QDs were successfully used for the study of in vitro and in vivo interaction of Trypanosoma cruzi and the triatomine Rhodnius prolixus. These QDs allowed us to acquire real time confocal images sequences of live T. cruzi-R. prolixus interactions for an extended period, causing no damage to the cells. By zooming to the region of interest, we have been able to acquire confocal images at the three to four frames per second rate. Our results show that QDs are physiological fluorescent markers capable to label living parasites and insect vector cells. QDs can be functionalized with lectins to specifically mark surface carbohydrates on perimicrovillar membrane of R. prolixus to follow, visualize, and understand interaction between vectors and its parasites in real-time.

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Section: Discussionmentioning

confidence: 97%

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

et al. 2009

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“…Moreover, QDs passivation or functionalization with thiol molecules [11–13] can be used as a universal bioconjugation. For example, mercaptoacetic acid (MAA), and mercaptosuccinic acid (MSA), link the thiol (-SH) termination with the CdTe QD, forming a small passivation layer of CdS around the nanoparticle.…”

Section: Introductionmentioning

confidence: 99%

“…QDs can be capped with chemically inert materials such as silica to protect the cell and the QD, avoiding both cell cytotoxicity, and chemical and/or metabolic degradation of the QD labeling inside the cells [ 10 ]. Moreover, QDs passivation or functionalization with thiol molecules [ 11 – 13 ] can be used as a universal bioconjugation. For example, mercaptoacetic acid (MAA), and mercaptosuccinic acid (MSA), link the thiol (-SH) termination with the CdTe QD, forming a small passivation layer of CdS around the nanoparticle.…”

Section: Introductionmentioning

confidence: 99%

CdTe and CdSe Quantum Dots Cytotoxicity: A Comparative Study on Microorganisms

Gomes

Vieira

Almeida

et al. 2011

Sensors

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Quantum dots (QDs) are colloidal semiconductor nanocrystals of a few nanometers in diameter, being their size and shape controlled during the synthesis. They are synthesized from atoms of group II–VI or III–V of the periodic table, such as cadmium telluride (CdTe) or cadmium selenium (CdSe) forming nanoparticles with fluorescent characteristics superior to current fluorophores. The excellent optical characteristics of quantum dots make them applied widely in the field of life sciences. Cellular uptake of QDs, location and translocation as well as any biological consequence, such as cytotoxicity, stimulated a lot of scientific research in this area. Several studies pointed to the cytotoxic effect against micoorganisms. In this mini-review, we overviewed the synthesis and optical properties of QDs, and its advantages and bioapplications in the studies about microorganisms such as protozoa, bacteria, fungi and virus.

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“…De Farias et al. reported the rapid cellular uptake of glucose‐capped NPs, less than 10 min in yeast cells, which express high‐affinity glucose transporters . In addition, the expression of GLUT1 and GLUT3 glucose transporters has been also observed in HeLa cells and other tumour cells …”

Section: Resultsmentioning

confidence: 99%

Glucose‐Modified Silicon Nanoparticles for Cellular Imaging

et al. 2017

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Luminescent silicon nanoparticles have recently attracted attention due to their remarkable stability, covalent functionalisation and tunable photoemission properties. Owing to their biocompatibility, low toxicity, and the small particle size that can be achieved by different synthetic approaches, these nanomaterials are candidates as cellular probes in the field of bioimaging, and potentially for in vivo applications. Tailoring the surface of the particles with active biomolecules such as sugar moieties can be an interesting strategy to increase the kinetics of internalisation or to vary the localisation of nanosystems in living cells. In this study, we synthesised and modified ultrasmall silicon nanoparticles with glucose covalently linked on their surface. Moreover, by varying the ratio between the amount of silicon nanoparticles and the saccharide groups, the amount of glucose, as a capping moiety, can be well controlled. FTIR spectroscopy, NMR spectroscopy, zeta potential measurements and anisotropy decay analysis confirmed the covalent binding of glucose to the nanoparticles. The photophysical behaviour of the surface-functionalised silicon quantum dots was not significantly different to that of the unmodified nanoparticles. In vitro studies demonstrated faster internalisation of the glucose-functionalised nanoparticles into HeLa cells. Different localisation and uptake kinetics of the glucose-modified particles compared to the unmodified particles are discussed in order to reveal the role played by the sugar molecules

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Highly fluorescent semiconductor core–shell CdTe–CdS nanocrystals for monitoring living yeast cells activity

Cited by 28 publications

References 15 publications

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

CdTe and CdSe Quantum Dots Cytotoxicity: A Comparative Study on Microorganisms

Glucose‐Modified Silicon Nanoparticles for Cellular Imaging

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