Highly Flexible Electrospun Hybrid (Polyurethane/Dextran/Pyocyanin) Membrane for Antibacterial Activity via Generation of Oxidative Stress

Sheet, Sunirmal; Vinothkannan, Mohanraj; Saravanakumar, B.; Subramaniyan, Sivakumar Allur; Acharya, Satabdi; Lee, Yang Soo

doi:10.1021/acsomega.8b01607

Cited by 18 publications

(10 citation statements)

References 52 publications

(99 reference statements)

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“…Additionally, phenazine-like redox-active molecules contribute to the survive of their producers under the anoxic or anaerobic conditions in honeybee guts [46]. Moreover, phenazines facilitate ROS accumulation to kill bacteria by oxidizing intracellular NADPH pool, and induce oxidative stress response, inflammatory response [25,47,48], consistent with the observation of accumulated ROS and increased inflammatory cytokines in duodenum in our work (Fig. 3C and Fig.…”

Section: Discussionsupporting

confidence: 88%

Phenazines Modulate Gastrointestinal Metabolism and Microbiota

Peng

Zhao

et al. 2021

Preprint

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Background: Natural and synthetic phenazines are ubiquitously occurred in environment and have been used for various therapeutic purposes in human, animals and agriculture, and the widespread use makes residue problem in environment and foods increasingly serious. However, the metabolic and comprehensive impacts of phenazines on the digestive tract are poorly understood, particularly the microbial pyocyanin (PYO), the most representative phenazines produced by Pseudomonas . Here, we utilized PYO as the representative of phenazines to study the effects on digestive tract. Results: Metabolic kinetic analysis showed that PYO exhibited low oral bioavailability in both rats and swine model, revealing a restriction of PYO in gut and might cause impacts on digestive tract. PYO was subsequently found to induce intestinal barrier destruction including inflammation and reactive oxygen species (ROS) accumulation in duodenum. Microbiome analysis showed that PYO caused gut microbiota dysbiosis by decreasing the symbiotic bacteria and increasing the opportunistic pathogenic bacteria. Additionally, the integral and dysfunctional assessment of liver demonstrated that PYO induced liver inflammation and metabolic disorder. Metabolism analysis further confirmed that PYO could be metabolized by both gut microbiota and liver, and all metabolites retained the nitrogen-containing tricyclic structural skeleton of phenazines, which was the core bioactivity of phenazine compounds, indicating all the outcomes were due to the intrinsic characteristic of phenazine structure. Conclusions: PYO were low oral bioavailable and all the metabolites retained the nitrogen-containing tricyclic structural skeleton, final resulting in the damages to digestive tract including intestinal barrier destruction, gut microbiota dysbiosis, liver damages and metabolic disorder. These findings elucidated the effect of phenazines on digestive tract in vivo and shed light on the rational design of phenazines for the development and application of such compounds in future.

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Section: Discussionsupporting

confidence: 88%

Phenazines Modulate Gastrointestinal Metabolism and Microbiota

Peng

Zhao

et al. 2021

Preprint

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“…In this case, also, a greater sensitivity of Gram-negative bacteria to these newly obtained materials was observed. In addition, the percentage of the bacterial inhibition of XG/PVA-based composite films containing hydro-alcoholic extract from Feteasca Neagra and Merlot grape pomace achieved onto Escherichia coli and Listeria monocytogenes ( Table 3 ) was superior to those already reported for other polysaccharide-based films incorporating quercetin or tannic acid [ 36 ] and were comparable only with those embedding pyocyanin [ 37 ]. Moreover, as far as we know, this is the first study which reports XG/PVA/Merlot pomace-based cryogels with an antimicrobial activity of 99% against Salmonella typhymurium .…”

Section: Resultsmentioning

confidence: 78%

A Comparative Analysis on the Effect of Variety of Grape Pomace Extracts on the Ice-Templated 3D Cryogel Features

Rǎschip¹,

Fifere²,

Dinu³

2021

Gels

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Nowadays, there is a growing interest in developing functional packaging materials from renewable resources containing bioactive compounds (such as polyphenols) in order to reduce the use of petroleum-based plastics and their impact on the environment. In this regard, the effect of a variety and concentration of grape pomace extracts (Feteasca Neagra or Merlot) incorporated within ice-templated 3D xanthan-based composites was evaluated by considering their water content, surface and texture properties, radical scavenging and microbiological activities. The embedding of Feteasca Neagra or Merlot grape pomace extracts was studied by static water swelling and contact angle measurements, and SEM, EDX, and TGA analyses. The water contact angle results showed an increase in the surface hydrophobicity of the extract-loaded cryogels with an increase in extract content from 10 to 40 v/v%. SEM micrographs indicated that the entrapment of grape pomace extracts affected the morphology of the pore walls and reduced the pore sizes. The antioxidant activity of grape pomace extract-loaded composite cryogels was closely related to the total phenolic content of grape variety and to their concentration into matrices. The highly hydrophobic character of composite cryogels containing Merlot grape pomace extract and their remarkable antimicrobial activity indicates a great potential of these materials for food packaging applications.

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“…Finally, the stained cells were counted using a BD FACSCalibur flow cytometer (San Jose, California, USA). The acquired data were isolated using FlowJo 10.0.7 software (Treestar Inc, Ashland, OR, US) [28].…”

Section: Methodsmentioning

confidence: 99%

1,4-Naphthoquinone Analogues: Potent Antibacterial Agents and Mode of Action Evaluation

et al. 2019

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1,4-Naphthoquinones have antibacterial activity and are a promising new class of compound that can be used to treat bacterial infections. The goal was to improve effective antibacterial agents; therefore, we synthesized a new class of naphthoquinone hybrids, which contain phenylamino-phenylthio moieties as significant counterparts. Compound 4 was modified as a substituted aryl amide moiety, which enhanced the antibacterial activity of earlier compounds 3 and 4. In this study, five bacterial strains Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae) were used to evaluate the antibacterial potency of synthesized naphthoquinones using the minimal inhibitory concentration (MIC) method. Most of the studied naphthoquinones demonstrated major antibacterial activity with a MIC of 15.6 µg/mL–500 µg/mL. Selected compounds (5a, 5f and 5x) were studied for the mode of action, using intracellular ROS generation, determination of apoptosis by the Annexin V-FITC/PI assay, a bactericidal kinetic study and in silico molecular modelling. Additionally, the redox potentials of the specified compounds were confirmed by cyclic voltammetry (CV).

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Highly Flexible Electrospun Hybrid (Polyurethane/Dextran/Pyocyanin) Membrane for Antibacterial Activity via Generation of Oxidative Stress

Cited by 18 publications

References 52 publications

Phenazines Modulate Gastrointestinal Metabolism and Microbiota

Phenazines Modulate Gastrointestinal Metabolism and Microbiota

A Comparative Analysis on the Effect of Variety of Grape Pomace Extracts on the Ice-Templated 3D Cryogel Features

1,4-Naphthoquinone Analogues: Potent Antibacterial Agents and Mode of Action Evaluation

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