Highly expressed TLX1NB and NPSR1-AS1 lncRNAs could serve as diagnostic tools in colorectal cancer

Dastjerdi, Shaghayegh; Valizadeh, Mehdi; Nemati, Reza; Honardoost, Mohammad Amin; Dolatabadi, Nasrin Fattahi; Zamani, Atefeh; Tabatabaeian, Hossein

doi:10.1007/s13577-021-00597-x

Cited by 8 publications

(7 citation statements)

References 21 publications

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“…Dastjerdi et al showed that NPSR1-AS1 had the ability to make a considerable distinction between the tumor and the normal samples. These findings might have repercussions for the early diagnosis and focused treatment of colorectal cancer in the future [ 36 ]. He et al discovered that NPSR1-AS1 activated the MAPK pathway to promote the proliferation and metastasis of thyroid cancer cells by engaging ELAVL1 to stabilize NPSR1 mRNA.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of NPSR1-AS1 as a Novel Diagnostic and Prognostic Biomarker Involved in Immune Infiltrates in Lung Adenocarcinoma

Zhang

Yuan

Xiang

et al. 2022

Journal of Oncology

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The incidence of lung adenocarcinoma (LUAD), the most common subtype of lung cancer, continues to make lung cancer the largest cause of cancer-related deaths worldwide. Long noncoding RNAs (lncRNAs) have been shown to have a significant role in both the onset and progression of lung cancer. In this study, we aimed to investigate the clinical significance and underlying mechanism of lncRNA NPSR1-AS1 (NPSR1-AS1) in LUAD. First, we performed an analysis on TCGA and identified 229 differentially expressed lncRNAs (DELs) (including 216 upregulated lncRNAs and 13 downregulated lncRNAs). Then, we carried out a screening of the lncRNAs associated with survival, and a total of 382 survival-related lncRNAs were found. 15 survival-related DELs were identified. Among them, our attention focused on NPSR1-AS1. We found that the expression of NPSR1-AS1 was much higher in LUAD specimens compared to nontumor tissues. According to the results of the ROC assays, high NPSR1-AS1 expression had an AUC value of 0.904 for LUAD, with a 95% confidence interval ranging from 0.881 to 0.927. The expression of NPSR1-AS1 was shown to be significantly elevated in a wide variety of cancers, according to the findings of a pancancer investigation. Functional enrichment analysis confirmed that NPSR1-AS1 was involved in LUAD progression via regulating several tumor-related pathways. Patients with high levels of NPSR1-AS1 expression were shown to have a shorter disease-specific survival (DSS) or overall survival (OS) than those with low levels of NPSR1-AS1 expression, according to the findings of a clinical investigation. It was determined by multivariate analysis that NPSR1-AS1 expressions served as an independent prognostic factor for the overall survival of LUAD patients. The results of immune cell infiltration revealed that the expressions of NPSR1-AS1 were negatively associated with CD8 T cells, pDC, cytotoxic cells, mast cells, iDC, neutrophils, NK CD56dim cells, DC, Th17 cells, Tgd, and macrophages, while they were positively associated with NK CD56bright cells and B cells. Overall, our findings revealed that NPSR1-AS1 could serve as a potential biomarker to assess the clinical outcome and immune infiltration level in LUAD.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of NPSR1-AS1 as a Novel Diagnostic and Prognostic Biomarker Involved in Immune Infiltrates in Lung Adenocarcinoma

Zhang

Yuan

Xiang

et al. 2022

Journal of Oncology

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“…Knockdown of ENST00000455974 inhibits the migration of colon cancer cells [ 24 ]. TLX1NB was recently reported to be upregulated in CRC tissues relative to that in adjacent normal tissues [ 8 ]. In addition, elevated TLX1NB levels were correlated with higher-grade CRC [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…TLX1NB was recently reported to be upregulated in CRC tissues relative to that in adjacent normal tissues [ 8 ]. In addition, elevated TLX1NB levels were correlated with higher-grade CRC [ 8 ]. However, the specific mechanistic role of TLX1NB in colon cancer remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…However, recent studies indicated that lncRNAs are involved in the invasion and metastasis of colon cancer [ 6 , 7 ]. The lncRNA T cell leukemia homeobox 1 neighbor (TLX1NB) is encoded in the human 10q24.31 chromosomal region and was reported to be upregulated in colorectal cancer (CRC) tissues [ 8 ]. Although elevated TLX1NB levels are correlated with high-grade CRC [ 8 ], the functional role of this lncRNA in colon cancer remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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The long non-coding RNA T cell leukemia homeobox 1 neighbor enhances signal transducer and activator of transcription 5A phosphorylation to promote colon cancer cell invasion, migration, and metastasis

et al. 2022

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Colon cancer is among the most prevalent gastrointestinal tumor types. The long noncoding RNA (lncRNA) T cell leukemia homeobox 1 neighbor (TLX1NB) is up-regulated in colorectal cancer (CRC). However, the functional role of this lncRNA in colon cancer remains unknown. In our study, we investigated the clinical significance of TLX1NB in colon cancer through bioinformatics analysis and explored its role in migration, invasion and metastasis of colon cancer cell with a series of experiments. Firstly, TLX1NB was up-regulated in colon cancer tissues and increased TLX1NB expression was significantly associated with advanced N stages. In wound healing assays and transwell assays, TLX1NB overexpression promoted HCT116 cell migration and invasion while TLX1NB knockdown inhibited SW620 cell migration and invasion. In vivo, TLX1NB knockdown suppressed pulmonary metastasis of SW620 cell and vimentin expression but increased E-cadherin expression. Then, TLX1NB overexpression enhanced signal transducer and activator of transcription 5A (STAT5A) phosphorylation and TLX1NB knockdown suppressed STAT5A phosphorylation. Moreover, the inhibition of STAT5A phosphorylation reversed TLX1NB overexpression-associated increase in HCT116 cell migratory and invasive activity. In conclusion, TLX1NB enhances STAT5A phosphorylation to promote colon cancer cell invasion, migration, and metastasis.

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“…The advent of deep sequencing methods, together with reliable annotation databases, has led to rapid progress in the understanding and identification of functional lncRNAs in different cancer types, including colorectal cancer 12 . In addition to the well‐known dysregulated lncRNAs in colorectal cancer, such as HOTAIR, 13 NEAT1 14 and H19, 15 recently, Differentiation Antagonizing Non‐Protein Coding RNA (DANCR), 16 Small Nucleolar RNA Host Gene 11 (SNHG11), 17 Embryonic Stem Cell Related (ESRG), Prader‐Willi Region Non‐Protein Coding RNA 1 (PWRN1) 18 and TLX1 Neighbour (TLX1NB) 19 have been identified as dysregulated lncRNAs in colorectal cancer. Long noncoding RNAs can be used to improve our understanding of cancer aetiology and can also be exploited as diagnostic biomarkers for early detection of cancer due to their stability and detectability in body fluids 20–22 …”

Section: Introductionmentioning

confidence: 99%

MIMT1 and LINC01550 are uncharted lncRNAs down‐regulated in colorectal cancer

Vejdandoust

Moosavi

Dolatabadi³

et al. 2023

Int J Experimental Path

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Incomplete knowledge of the molecular basis of colorectal cancer, with subsequent limitations in early diagnosis and effective treatment, has contributed to this form of malignancy becoming the second most common cause of cancer‐related death worldwide. With the advances in high‐throughput profiling techniques and the availability of public data sets such as The Cancer Genome Atlas Program (TCGA), a broad range of coding transcripts have been profiled and their underlying modes of action have been mapped. However, there is still a huge gap in our understanding of noncoding RNA dysregulation. To this end, we used a bioinformatics approach to shortlist and evaluate yet‐to be‐profiled long noncoding RNAs (lncRNAs) in colorectal cancer. We analysed the TCGA RNA‐seq data and followed this by validating the expression patterns using a qPCR technique. Analysing in‐house clinical samples, the real‐time PCR method revealed that the shortlisted lncRNAs, that is MER1 Repeat Containing Imprinted Transcript 1 (MIMT1) and Non‐Protein Coding RNA 1550 (LINC01550), were down‐regulated in colorectal cancer tumours compared with the paired adjacent normal tissues. Mechanistically, the in silico results suggest that LINC01550 could form a complex competitive endogenous RNA (ceRNA) network leading to the subsequent regulation of colorectal cancer‐related genes, such as CUGBP Elav‐Like Family Member (CELF2), Polypyrimidine Tract Binding Protein 1 (PTBP1) and ELAV Like RNA Binding Protein 1 (ELAV1). The findings of this work indicate that MIMT1 and LINC01550 could be novel tumour suppressor genes that can be studied further to assess their roles in regulating the cancer signalling pathway(s).

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Highly expressed TLX1NB and NPSR1-AS1 lncRNAs could serve as diagnostic tools in colorectal cancer

Cited by 8 publications

References 21 publications

Comprehensive Analysis of NPSR1-AS1 as a Novel Diagnostic and Prognostic Biomarker Involved in Immune Infiltrates in Lung Adenocarcinoma

Comprehensive Analysis of NPSR1-AS1 as a Novel Diagnostic and Prognostic Biomarker Involved in Immune Infiltrates in Lung Adenocarcinoma

The long non-coding RNA T cell leukemia homeobox 1 neighbor enhances signal transducer and activator of transcription 5A phosphorylation to promote colon cancer cell invasion, migration, and metastasis

MIMT1 and LINC01550 are uncharted lncRNAs down‐regulated in colorectal cancer

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