Highly Efficient Spectrophotometric Determination of Cu2+ ion in Aqueous Medium Using a thiosemicarbazone–derivative Ligand

Başoğlu, Aysel; Ocak, Ümmühan; Karaküçük‐İyidoğan, Ayşegül

doi:10.1007/s10895-022-03127-6

Cited by 1 publication

(1 citation statement)

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“…21a ), it can be attributed to the extension of the electron conjugation system by the chelate structure formed after metal ion coordinated with the ligand, resulting in the emergence of new absorption peaks at longer wavelengths 36 . In addition, an iso-absorption point (~330 nm) was observed between the peak maximum of Cu 1 R 5 (312 nm) and the peak maximum of the complex (386 nm), suggesting a single equilibrium step 37 . More importantly, when Cu + concentration increased from 0 to 20.0 μM, the intensity of the absorption band around 386 nm was gradually increased.…”

Section: Resultsmentioning

confidence: 95%

Alkyne-tagged SERS nanoprobe for understanding Cu+ and Cu2+ conversion in cuproptosis processes

Zhang,

Mei,

Liu

et al. 2024

Nat Commun

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Simultaneously quantifying mitochondrial Cu+ and Cu2+ levels is crucial for evaluating the molecular mechanisms of copper accumulation-involved pathological processes. Here, a series of molecules containing various diacetylene derivatives as Raman reporters are designed and synthesized, and the alkyne-tagged SERS probe is created for determination Cu+ and Cu2+ with high selectivity and sensitivity. The developed SERS probe generates well-separated distinguishable Raman fingerprint peaks with built-in corrections in the cellular silent region, resulting in accurate quantification of Cu+ and Cu2+. The present probe demonstrates high tempo-spatial resolution for real-time imaging and simultaneously quantifying mitochondrial Cu+ and Cu2+ with long-term stability benefiting from the probe assembly with designed Au-C≡C groups. Using this powerful tool, it is found that mitochondrial Cu+ and Cu2+ increase during ischemia are associated with breakdown of proteins containing copper as well as conversion of Cu+ and Cu2+. Meanwhile, we observe that parts of Cu+ and Cu2+ are transported out of neurons by ATPase. More importantly, cuproptosis in neurons is found including the oxidative stress process caused by the conversion of Cu+ to Cu2+, which dominates at the early stage (<9 h), and subsequent proteotoxic stress. Both oxidative and proteotoxic stresses contribute to neuronal death.

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Section: Resultsmentioning

confidence: 95%