HIGHLY EFFICIENT PRODUCTION OF L (+)-LACTIC ACID USING MEDIUM WITH POTATO, CORN STEEP LIQUOR AND CALCIUM CARBONATE BY Lactobacillus rhamnosus ATCC 9595

Abstract

The objective of this study was to optimize the composition of the fermentation medium through a Central Composite Rotational Design (CCRD) for the production of L (+)-latic acid (LA) using Lactobacillus rhamnosus ATCC 9595 and potato, corn steep liquor (CSL) and calcium carbonate as raw materials. A CCRD with three independent variables (potato flour (PF), CSL and CaCO 3 concentrations) was performed for a total of 17 treatments. Kinetic studies were conducted, and samples were taken at time intervals of 0 to 72 h. Cell count, pH, reducing sugars and LA concentrations were determined. LA productivity and yield, sugar consumption and cell growth factor were calculated. The highest concentration of LA was 183.8 g/L, with 2.553 g/L.h productivity, 94.2% yield and 96.1 % sugar consumption.

Improvement of Polymer Grade L-Lactic Acid Production Using Lactobacillus rhamnosus SCJ9 from Low-Grade Cassava Chips by Simultaneous Saccharification and Fermentation

“…L. rhamnosus has been extensively studied for various applications particularly the use as probiotic against diarrhea and arthritis [28]. This bacterial species was commonly found to remarkable tolerance when exposing to the harsh acidic condition which is a typical condition in the stomach and digestive tract [29,30] and it has also been used in lactic acid production [10,31]. Regarding the fast-growing and highly optically pure L-lactic acid-producing capabilities, not only the application in L-lactic acid production, a newly isolated L. rhamnosus SCJ9 may provide the suitable characteristics for the use as a probiotic in the food and feed industries.…”

“…Pretreatment with cellulosic material has been found to cause several technical problems such as inhibition of the enzyme involved in cellulose hydrolysis by intermediate products. On the contrary, various biomass materials in the form of starch such as corn, cassava, potato, barley, and wheat have been used as carbon sources for the production of lactic acid [10][11][12]. In addition, starch-containing wastes have also been reportedly used as the main substrate for lactic acid fermentation [13,14].…”

l‐Lactate oxidase‐mediated removal of l‐lactic acid derived from fermentation medium for the production of optically pure D‐lactic acid

“…De Cunha et al. [ 19 ] reported that Lacticaseibacillus rhamnosus ATCC 9595 could produce 183.8 g L –1 of lactic acid using 220 g L –1 of potato flour and 55 g L –1 of CSL. Although not mentioned in these reports, CSL contains a large amount of lactic acid, consisting of a mixture of l ‐lactic and d ‐lactic acids.…”

“…of CSL could reduce the concentration of yeast extract to 1 g L -1 in L-lactic acid fermentation from starch hydrolysate using Enterococcus faecalis RKY1. De Cunha et al [19] reported that Lacticaseibacillus rhamnosus ATCC 9595 could produce 183.8 g L -1 of lactic acid using 220 g L -1 of potato flour and 55 g L -1 of CSL. Although not mentioned in these reports, CSL contains a large amount of lactic acid, consisting of a mixture of L-lactic and D-lactic acids.…”

“…As an alternative cheap nitrogen source, many authors have demonstrated the utility of corn steep liquor (CSL), which is occurred in starch manufacturing process from corns [ 16 ] in lactic acid production. [ 17–20 ] Wee et al. [ 18 ] reported that the addition of 30 g L –1 of CSL could reduce the concentration of yeast extract to 1 g L –1 in l ‐lactic acid fermentation from starch hydrolysate using Enterococcus faecalis RKY1.…”