Highly efficient production of 1-cyanocyclohexaneacetic acid by cross-linked cell aggregates (CLCAs) of recombinant E. coli harboring nitrilase gene

Zou, Shu-Ping; Huang, Jiuzuo; Xue, Ya‐Ping; Zheng, Yu‐Guo

doi:10.1016/j.procbio.2017.10.014

Cited by 21 publications

(1 citation statement)

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“…Cell immobilization is often regarded as an appropriate strategy for efficient industrial production, , since the utilization of immobilized cells not only enhances the reusability of biocatalysts but also simplifies the separation of products. − In addition, immobilized cells have better catalytic performances (thermal stability, pH variation, or activity) than free cells − and easily adapt to the difference between the cellular environment and the industrial setting. , …”

Section: Introductionmentioning

confidence: 99%

Efficient Chemoenzymatic Synthesis of Optically Active Pregabalin from Racemic Isobutylsuccinonitrile

Zhang

Liu

et al. 2019

Org. Process Res. Dev.

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An efficient chemoenzymatic route has been developed for the synthesis of optically active pregabalin (PGB) from isobutylsuccinonitrile (IBSN). (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), a critical chiral intermediate of PGB, was synthesized using regio- and enantioselective hydrolysis of IBSN by immobilized Escherichia coli cells harboring nitrilase BrNIT from Brassica rapa. The catalytic performances of immobilized cells were investigated, and high enantioselectivity (E > 150) and substrate conversion (>41.1%) were obtained at a substrate loading of 100 g/L by immobilized cells after 12 batches of reaction. The unreacted (R)-IBSN was recycled by racemization with a high yield of 94.5%, and the resultant (S)-CMHA was hydrogenated directly to the desired PGB with a high purity of 99.6% and optical purity of 99.4%. The input of raw materials and E factor of this chemoenzymatic route were demonstrated to be much lower than those of the first- and second-generation routes for PGB synthesis.

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