Highly efficient preparation of 1-lysophosphatidylcholine via high proportion of Novozym® 435 (lipase B from Candida antarctica)-catalyzed ethanolysis

Yasuda, Sayumi; Yamamoto, Yukihiro

doi:10.1016/j.btre.2020.e00505

Cited by 2 publications

(2 citation statements)

References 19 publications

(26 reference statements)

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“…Sarney et al [ 63 ], for example, tested the transesterification reaction in a range of alcohols and reaction conditions (time and temperature) using immobilized R. miehei lipase. More recently, Mnasri et al [ 64 ] compared multiple enzymes ( R. miehei , T. lanuginosus , Candida antarctica ) and reaction conditions (substrate ratio, amount of catalyst and temperature), while Yasuda and Yamamoto [ 65 ] prepared LPC using lipase B from C. antarctica (Novozym ® 435).…”

Section: Resultsmentioning

confidence: 99%

New Forms of Neuroactive Phospholipids for DHA Enrichment in Brain

Gomes,

Mendes,

Duarte

et al. 2024

Marine Drugs

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Low levels of docosahexaenoic acid (DHA) in the brain have been related to neurological disorders, like Alzheimer’s disease (AD). After ingestion, dietary DHA must cross the blood–brain barrier, where it is absorbed as lysophosphatidylcholine (LPC), due to its role as a preferential DHA carrier in the brain. This work aimed at the production of LPC-DHA extracts to be used in supplementation/food fortification intended neural enrichment in DHA. As it is rich in DHA, especially its phospholipids (PL), Atlantic mackerel (Scomber scombrus, caught in Spring/2022) was used as a raw material. The polar lipids fraction was separated and hydrolysed with Rhizomucor miehei lipase, to enzymatically convert phosphatidylcholine (PC) into LPC. The fish (muscle and by-products) lipids fraction was used for total lipids (TL) content, lipid classes (LC) and fatty acid (FA) profile evaluation, whilst polar lipids extracts were studied for LC production and FA analysis. Muscle TL ranged between 1.45 and 4.64 g/100 g (WW), while by-products accounted for 7.56-8.96 g/100 g, with the highest contents being found in March. However, PL were more abundant in muscle (22.46–32.20% of TL). For polar lipids extracts, PL represented 50.79% of TL, among which PC corresponded to 57.76% and phosphatidylethanolamine to 42.24%. After hydrolysis, nearly half of this PC was converted into LPC. When compared to the initial PC, DHA relative content (33.6% of total FA) was significantly higher after hydrolysis: 55.6% in PC and 73.6% in LPC. Such extract, obtained from this undervalued species, may represent a promising strategy to increase DHA uptake into brain cells while allowing this species to upgrade.

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Section: Resultsmentioning

confidence: 99%

New Forms of Neuroactive Phospholipids for DHA Enrichment in Brain

Gomes,

Mendes,

Duarte

et al. 2024

Marine Drugs

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“…Phosphatidylcholines consist of a polar phosphocholine head group and glycerophosphoric acid, along with a variety of fatty acids. Based on published and validated data regarding the lipid composition of bovine lung surfactant, applying techniques for resolving fatty acid chains, it is suggested that the fatty acid chains of LPC in bovine lung surfactant are predominantly palmitic acid (C16:0) and monoenoic species, with oleic acid (9C-18:1) being particularly abundant (Barrow, 1990;Dong et al, 2010;Haynes & De Jesús, 2012;Hedger, 2010;Heinzelmann et al, 2014;Kosicek et al, 2010;Law et al, 2019;Léon et al, 2004;Mehling et al, 2013;Rehman et al, 2017;Schmitz & Ruebsaamen, 2010;Shelley et al, 1984;Wong et al, 2022;Yasuda & Yamamoto, 2020;Yoder et al, 2014;Yu et al, 1983;Zhang et al, 2016;Zhou et al, 2019). Some research has been conducted to analyze LPC using liquid chromatography.…”

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confidence: 99%

Validation of a high‐performance liquid chromatography method for determining lysophosphatidylcholine content in bovine pulmonary surfactant medication

Aghaei,

Talari,

Mollahosseini

et al. 2024

Biomedical Chromatography

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Pulmonary surfactant replacement therapy is a promising improvement in neonatal care for infants with respiratory distress syndrome. Lysophosphatidylcholine (LPC) is an undesirable component that can hinder surfactant proteins from enhancing the adsorption of surfactant lipids to balance surface tensions by creating a saturated coating on the interior of the lungs. A novel normal‐phase liquid chromatography method utilizing UV detection and non‐toxic solvents was developed and validated for the first time to analyze LPC in the complex matrix of pulmonary surfactant medication. The analytical method validation included evaluation of system suitability, repeatability, intermediate precision, linearity, accuracy, limit of detection (LOD), limit of quantification (LOQ), stability and robustness. The method yielded detection and quantification limits of 4.4 and 14.5 μg/ml, respectively. The calibration curve was modified linearly within the LOQ to 1.44 mg/ml range, with a determination coefficient of 0.9999 for standards and 0.9997 for sample solutions. Given the lack of reliable published data on LPC analysis in pulmonary surfactant medications, this newly developed method demonstrates promising results and offers advantages of HPLC methodology, including simplicity, accuracy, specificity, sensitivity and an exceptionally low LOD and LOQ. These attributes contribute to considering this achievement as an innovative method.

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Highly efficient preparation of 1-lysophosphatidylcholine via high proportion of Novozym® 435 (lipase B from Candida antarctica)-catalyzed ethanolysis

Cited by 2 publications

References 19 publications

New Forms of Neuroactive Phospholipids for DHA Enrichment in Brain

New Forms of Neuroactive Phospholipids for DHA Enrichment in Brain

Validation of a high‐performance liquid chromatography method for determining lysophosphatidylcholine content in bovine pulmonary surfactant medication

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