Highly Efficient Intramolecular Electrochemiluminescence Energy Transfer for Ultrasensitive Bioanalysis of Aflatoxin M1

Abstract

The intermolecular electrochemiluminescence resonance energy transfer (ECL-RET) between luminol and Ru(bpy) was studied extensively to achieve the sensitive bioanalysis owing to the perfect spectral overlap of the donor and acceptor, but it still suffers from the challenging issue of low energy-transfer efficiency. The intramolecular ECL-RET towards the novel ECL compound containing the donor of luminol and the acceptor of Ru(bpy) (mcpbpy) (Lum-Ru) was designed and investigated. With the high-efficient ECL-RET in one molecule, the highly intense ECL signal of Lum-Ru was obtained owing to the short path of energy transmission and less energy loss between luminol and Ru(bpy) (mcpbpy) . Lum-Ru was further applied to construct a signal-off electrochemiluminescence (ECL) aptasensor for ultrasensitive detection of a harsh carcinogen of Aflatoxin M1 (AFM1). This sensing platform also provides a significant boost for the trace detection of other biomolecules in clinical analysis.

Dual resonance energy transfer in triple-component polymer dots to enhance electrochemiluminescence for highly sensitive bioanalysis

“…By incorporating Ru(bpy) 3 2+ into Pdots, the RET from the excited Pdots to encapsulated Ru(bpy) 3 2+ can enhance the ECL emission by 15.7 fold 24. The intramolecular RET achieved by integrating luminol (donor) and Ru(bpy) 2 (mcpbpy) 2+ (acceptor) into one molecule has showed enhanced ECL emission owing to the short path of energy transmission and less energy loss 25. In this work, triple-component (D1, A1/D2 and A2) Pdots ( P1 dots) are composited with carbazole, tetraphenylethene (TPE), and 2,2-difluoro-3-(4-methoxyphenyl)-4,6-diphenyl-2 H -1,3l4,2l4-oxazaborinine (DMDO) (Scheme 1A).…”

Synthesis and Chemiluminescent Properties of Amino-Acylated luminol Derivatives Bearing Phosphonium Cations

“…Their synthesis was reportedly performed via the direct acylation of luminol with acyl chlorides, while their CL efficiencies appeared to be much lower to those of luminol, indicating that chemical functionalization can substantially alter the CL properties of the parent molecule. On the other hand, highly-efficient (electro)chemiluminescent luminol-Ru(bpy) 3 donor-acceptor dyads [35,36] have been lately prepared from the direct acylation of luminol, while acylation has very recently been also achieved using cyclic anhydrides [37]. Thus, our goal was two-fold, namely: a) the development of an efficient synthetic procedure for a series of luminol and isoluminol amino-acylated phosphonium derivatives; and b) the evaluation of the chemiluminescence properties of these derivatives, in order to assess their potential for in vivo CL performance.…”

Mycotoxins Detection Based on Electrochemical Approaches

“…Owing to the enhancement of the effective collision efficiency and reduction of steric energy loss, appropriate donor-acceptor pairs of RET can improve ECL performance. For instance, the excited state of luminol (AP 2 À * ) used as donor and Ru as acceptor, high-intense ECL response achieved via ECL-RET in the Lum-Ru intramolecular (Figure 7D) [125]. AFM1 could be recycled by means of one-pot target-induced circular exponential amplification strategy, while a large amount of ferrocenelabeled dsDNA formed to quenching ECL signal.…”