Highly efficient in vivo mutagenesis method based on the endonuclease and exonuclease activity of Bacillus alcalophilus RecJ (BaRecJ)
Jixiang Shang,
Yanchao Zhang,
Zongjun Xu
et al.
Abstract:With the continual advancement of technologies such as microbial cultivation, DNA sequencing, bioinformatics, and genetic engineering, methods for microbial breeding have become increasingly diverse. We identified a RecJ enzyme (BaRecJ) with both endonuclease and exonuclease activities from B. alcalophilus. Differing from traditional physical-chemical mutagenesis approaches and the methods based on perturbation factor. this research established a novel mutagenesis method utilizing the endonuclease and exonucle… Show more
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