Highly efficient gene transfer in naive human T cells with a murine leukemia virus-based vector

Dardalhon, Valérie; Jaleco, Sara; Rebouissou, Cosette; Ferrand, Christophe; Skander, Nadia; Swainson, Louise; Tiberghien, Pierre; Spits, Hergen; Noraz, Nelly; Taylor, Naomi

doi:10.1182/blood.v96.3.885

Cited by 24 publications

(2 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies have reported that IL-7 does not alter the phenotype of naïve lymphocytes and has no effect on activation markers. 39,[46][47][48] Others have observed that IL-7 induces the up-regulation of certain activation markers (CD25/CD95) but because these changes were not accompanied by conversion of the cells from CD45RA + to CD45RO + phenotype, these changes were classified as minimal. 40,41 In our study, we included three separate controls (untreated, IL-2-treated, and fresh) with the realization that each has its own limitations.…”

Section: Discussionmentioning

confidence: 99%

Interleukin‐7 signalling is sufficient to phenotypically and functionally prime human CD4⁺ naïve T cells

2005

View full text Add to dashboard Cite

Summary Interleukin‐7 (IL‐7) is produced by bone marrow and lymphoid stromal cells and is involved in the synthesis, survival and homeostasis of T cells. These attributes are the basis for current strategies to utilize IL‐7 as an immune modulator for several clinical conditions to replenish depleted T‐cell numbers. Because we had previously determined that IL‐7 can induce potent human immunodeficiency virus replication in the otherwise non‐permissive CD4+ naïve T‐cell compartment, we evaluated here the impact of IL‐7 on the phenotype and functional potential of naïve CD4+ T cells in an attempt to understand the mechanism of this induction. We demonstrate that IL‐7 mediated the up‐regulation of CD25, CD95 and human leucocyte antigen‐DR, while it did not alter the expression of CD45RO, CD69, CD40, or CD154. Examination of the cytokine profile of IL‐7‐treated naïve T cells using a Type1/Type2 Proteome Array indicated a remarkable IL‐7‐mediated induction of interferon‐γ production, while the other cytokines evaluated (IL‐2, IL‐12, tumour necrosis factor‐α, IL‐4, IL‐5, IL‐10 and IL‐13) were not affected. Intracellular staining of IL‐7‐treated naïve T cells for interferon‐γ verified the Proteome data. IL‐7 did not induce cell cycle proliferation of naïve CD4+ T cells, as evaluated by 7‐AAD/pyronin immunostaining and carboxyfluorescein diacetate succinimidyl ester dye tracking. IL‐7 treatment of naïve CD4+ T cells induced their ability to prime monocytes, as was indicated by induction of CD80 and CD86 expression on monocytes cocultured with IL‐7‐treated naïve CD4+ T cells. Collectively, these data indicate that IL‐7 signalling is sufficient to phenotypically and functionally prime human CD4+ naïve T cells independent of antigen stimulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Interleukin‐7 signalling is sufficient to phenotypically and functionally prime human CD4⁺ naïve T cells

2005

View full text Add to dashboard Cite

show abstract

“…studied. Dardalhon et al (2000) used a retroviral vector encoding eGFP and packaged with GALV to transduce mature PBMC and T cells from umbilical cord collections. Naive umbilical cord T cells were shown to switch from CD45RA to the CD45RO phenotype after 6 d of stimulation, and were transduced at lower efficiency than mature T cells.…”

Section: Discussionmentioning

confidence: 99%

The impact of retroviral suicide gene transduction procedures on T cells

et al. 2003

View full text Add to dashboard Cite

Summary. Retroviral vectors encoding the herpes simplex thymidine kinase gene have been used to render T cells sensitive to the prodrug ganciclovir. Such genetically modified T cells have been used in clinical trials for their graft-versus-leukaemia effects following allogeneic haematopoietic stem cell transplantation. In the event of graftversus-host disease (GVHD) the cells were susceptible to elimination through exposure to ganciclovir. We have investigated the impact of T-cell activation, required for successful retrovirus-mediated gene transfer, on T-cell receptor repertoire profile, subset distribution and antiviral potential. Using a combination of antibodies against CD3 and CD28, T cells were transduced at high efficiency when exposed to retrovirus between 48 and 72 h later.Lymphocytes had undergone up to seven cycles of cell division by the end of the procedure. Although the T-cell receptor Vb repertoire was not altered after retroviral transduction, there were notable shifts in subset profiles with an increased proportion of CD45RO cells in transduced populations. T cells continued to proliferate for several days after transduction and were difficult to sustain under the extended culture conditions required to generate virus-specific T cells. These observations may explain the lower than expected levels of GVHD and poor antiviral immunity reported in recent trials.

show abstract

Cell Isolation and Expansion Using Dynabeads ®

Neurauter¹,

Bonyhadi²,

Lien³

et al.

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

This chapter describes the use of Dynabeads for cell isolation and expansion. Dynabeads are uniform polystyrene spherical beads that have been made magnetisable and superparamagnetic, meaning they are only magnetic in a magnetic field. Due to this property, the beads can easily be resuspended when the magnetic field is removed. The invention of Dynabeads made, by Professor John Ugelstad, has revolutionized the separation of many biological materials. For example, the attachment of target-specific antibodies to the surface of the beads allows capture and isolation of intact cells directly from a complex suspension such as blood. This is all accomplished under the influence of a simple magnetic field without the need for column separation techniques or centrifugation. In general, magnetic beads coated with specific antibodies can be used either for isolation or depletion of various cell types. Positive or negative cell isolation can be performed depending on the nature of the starting sample, the cell surface markers and the downstream application in question. Positive cell isolation is the method of choice for unprocessed samples, such as whole blood, and for downstream molecular applications. Positive cell isolation can also be used for any downstream application after detachment and removal of the beads. Negative cell isolation is the method of choice when it is critical that cells of interest remain untouched, i.e., no antibodies have been bound to any cell surface markers on the cells of interest. Some cell populations can only be defined by multiple cell surface markers. Such populations of cells can be isolated by the combination of negative and positive cell isolation. By coupling Dynabeads with antibodies directed against cell surface activation molecules, the beads can be used both for isolation and expansion of the cells. Dynabeads are currently used in two major clinical applications: 1) In the Isolex 300i Magnetic Cell Selection System for CD34 Stem Cell Isolation--2) For ex vivo T cell isolation and expansion using Dynabeads ClinExVivo CD3/CD28 for clinical trials in novel adoptive immunotherapy.

show abstract

Highly efficient gene transfer in naive human T cells with a murine leukemia virus-based vector

Cited by 24 publications

References 61 publications

Interleukin‐7 signalling is sufficient to phenotypically and functionally prime human CD4⁺ naïve T cells

Interleukin‐7 signalling is sufficient to phenotypically and functionally prime human CD4⁺ naïve T cells

The impact of retroviral suicide gene transduction procedures on T cells

Cell Isolation and Expansion Using Dynabeads ®

Contact Info

Product

Resources

About

Highly efficient gene transfer in naive human T cells with a murine leukemia virus-based vector

Cited by 24 publications

References 61 publications

Interleukin‐7 signalling is sufficient to phenotypically and functionally prime human CD4+ naïve T cells

Interleukin‐7 signalling is sufficient to phenotypically and functionally prime human CD4+ naïve T cells

The impact of retroviral suicide gene transduction procedures on T cells

Cell Isolation and Expansion Using Dynabeads ®

Contact Info

Product

Resources

About

Interleukin‐7 signalling is sufficient to phenotypically and functionally prime human CD4⁺ naïve T cells

Interleukin‐7 signalling is sufficient to phenotypically and functionally prime human CD4⁺ naïve T cells