Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell tracking

Abstract

Tracking the distribution of stem cells is crucial to their therapeutic use. However, the usage of current vectors in cellular labeling is restricted by their low internalizing efficiency. Here, we reported a cellular labeling approach with a novel vector composed of mesoporous silica nanoparticles (MSNs) conjugated with fluorescein isothiocyanate in human bone marrow mesenchymal stem cells and 3T3-L1 cells, and the mechanism about fluorescein isothiocyanate-conjugated MSNs (FITC-MSNs) internalization was studied. FITC-MSNs were efficiently internalized into mesenchymal stem cells and 3T3-L1 cells even in short-term incubation. The process displayed a time- and concentration-dependent manner and was dependent on clathrin-mediated endocytosis. In addition, clathrin-dependent endocytosis seemed to play a decisive role on more internalization and longer stay of FITC-MSNs in mesenchymal stem cells than in 3T3-L1 cells. The internalization of FITC-MSNs did not affect the cell viability, proliferation, immunophenotype, and differentiation potential of mesenchymal stem cells, and 3T3-L1 cells. Finally, FITC-MSNs could escape from endolysosomal vesicles and were retained the architectonic integrity after internalization. We conclude that the advantages of biocompatibility, durability, and higher efficiency in internalization suit MSNs to be a better vector for stem cell tracking than others currently used.

Interaction of SiO2 nanoparticles with neuronal cells: Ionic mechanisms involved in the perturbation of calcium homeostasis

“…While they are considered to be among the most biocompatible NPs (Huang et al, 2005;Izak-Nau et al, 2014;Kim et al, 2006), extensive knowledge of the cellular responses elicited by their interaction is still incomplete. This is of particular relevance for the nervous system (NS): nanoneuromedicine is rapidly gaining momentum (Gendelman et al, 2014),and silica NPs are good candidates for applications in this field, ranging from drug delivery (Rosenholmet al, 2011) to cell tracking and subcellular imaging (Montalti et al, 2014).…”

Adsorption at cell surface and cellular uptake of silica nanoparticles with different surface chemical functionalizations: impact on cytotoxicity

“…This is why current in vivo and in vitro researches are ongoing for a better knowledge of nanoparticles fate and to provide safer uses (Seaton and Donaldson 2012). Nanoparticles whose size is close to that of cellular components, may interact specifically with cells (Huang et al 2005). They can adhere to the cell membrane or be entirely engulfed by different pathways, especially by macrophage cells due to their phagocytic capacity (Leclerc et al 2012).…”

Magnetic Nanoparticles: Its Effect on Cellular Behaviour and Potential Applications

“…The mechanism of MNPs uptake by different kinds of stem cells are not fully investigated but recent study shows endocytosis by clathrin receptor is one of the mechanisms (Huang et al, 2005;Lu et al, 2007). These study shows inhibitor of clathrin receptor, phenylarsine oxide, can block the ingestion of mesoporous iron oxide nanoparticles into human mesenchymal stem cells.…”