Highly effective and inexpensive parasitological technique for diagnosis of intestinal parasites in developing countries: spontaneous sedimentation technique in tube

Abstract

Millions of low-income people in the world are affected by intestinal parasites. Inexpensive, simple, and effective techniques for diagnosis are necessary. The spontaneous sedimentation technique in tube (SSTT), for application in poor healthcare settings and under field-work conditions, was described 25 years ago in Peru by Tello. The advantages of the SSTT are its ability to detect the majority of intestinal parasites, including eggs, larvae, cysts, and trophozoites, and its low cost.

The Parasitic Contamination of Raw Vegetables Consumed in Zahedan, Iran

“…The suspension was strained through a sterile sieve to remove undesirable materials (14). The filtrate was centrifuged at 5000 rpm for 5 minutes (12), and the supernatant was discarded into the disinfectant jar.…”

Novel approaches to the diagnosis of Strongyloides stercoralis infection

“…1) prevails, the larval load in stools may be undetectable [13,21,22]. Faecal surveys on STHs not aiming at S. stercoralis with specific techniques miss most infections, and, as a consequence, the global and local burdens of strongyloidiasis are grossly underestimated [1,2,9,23]. Prevalence figures would probably be different (but still far from reality) if multiple sampling were to be used [12], but this is logistically difficult to implement in epidemiological studies.…”

Factors affecting the parasitic contamination of edible locally produced dry season leafy vegetables cultivated in south east Enugu, Nigeria

“…The filtrate was used to carry out both sedimentation and floatation techniques on each of the soil samples. The preparation was examined under the light microscope for various stages of intestinal parasites such as helminth eggs, larvae, cysts and protozoan using x10 and x40 objectives [8,10]. The processed samples from vegetables, soil and water that were not centrifuged, were used to carry out the floatation technique according to standard method [11], especially for the sand samples.…”

“…According to Abougraina et al [10], the most likely hypothesis of contamination is that it must have occurred before harvest, either by contaminated manure, manure compost, sewage sludge, irrigation water, runoff water from livestock operations or directly from wild and domestic animals. These potential contaminants are all plausible and consistent with the assumption that the level of contamination must have been high.…”