Highly dispersed CuO nanoparticles prepared by a novel quick-precipitation method

Zhu, Junwu; Li, Dan; Chen, Haiqun; Yang, Xin; Lu, Lude; Wang, Xin

doi:10.1016/j.matlet.2004.06.031

Cited by 269 publications

(137 citation statements)

References 12 publications

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“…CuONP was synthesized as Zhu et al reported, 21 and characterized using transmission electron microscope H-7650 (Hitachi Ltd., Tokyo, Japan), a Malvern Zetasizer Nano ZS90 (Malvern Instruments, Malvern, UK), and X-ray Photoelectron Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) ( Figure 1 cell culture and treatment…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

Activation of Erk and p53 regulates copper oxide nanoparticle-induced cytotoxicity in keratinocytes and fibroblasts

Luo¹,

Li²,

Yang³

et al. 2014

IJN

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Copper oxide nanoparticles (CuONP) have attracted increasing attention due to their unique properties and have been extensively utilized in industrial and commercial applications. For example, their antimicrobial capability endows CuONP with applications in dressings and textiles against bacterial infections. Along with the wide applications, concerns about the possible effects of CuONP on humans are also increasing. It is crucial to evaluate the safety and impact of CuONP on humans, and especially the skin, prior to their practical application. The potential toxicity of CuONP to skin keratinocytes has been reported recently. However, the underlying mechanism of toxicity in skin cells has remained unclear. In the present work, we explored the possible mechanism of the cytotoxicity of CuONP in HaCaT human keratinocytes and mouse embryonic fibroblasts (MEF). CuONP exposure induced viability loss, migration inhibition, and G 2 /M phase cycle arrest in both cell types. CuONP significantly induced mitogen-activated protein kinase (extracellular signal-regulated kinase [Erk], p38, and c-Jun N-terminal kinase [JNK]) activation in dose- and time-dependent manners. U0126 (an inhibitor of Erk), but not SB 239063 (an inhibitor of p38) or SP600125 (an inhibitor of JNK), enhanced CuONP-induced viability loss. CuONP also induced decreases in p53 and p-p53 levels in both cell types. Cyclic pifithrin-α, an inhibitor of p53 transcriptional activity, enhanced CuONP-induced viability loss. Nutlin-3α, a p53 stabilizer, prevented CuONP-induced viability loss in HaCaT cells, but not in MEF cells, due to the inherent toxicity of nutlin-3α to MEF. Moreover, the experiments on primary keratinocytes are in accordance with the conclusions acquired from HaCaT and MEF cells. These data demonstrate that the activation of Erk and p53 plays an important role in CuONP-induced cytotoxicity, and agents that preserve Erk or p53 activation may prevent CuONP-induced cytotoxicity.

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Section: Materials and Methods Materialsmentioning

confidence: 99%

Activation of Erk and p53 regulates copper oxide nanoparticle-induced cytotoxicity in keratinocytes and fibroblasts

Luo¹,

Li²,

Yang³

et al. 2014

IJN

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“…[12][13][14][15][16][17][18][19][20] Therefore, it was a challenge to find a convenient, mild, nontoxic, natural product to produce metal nanoparticles in an aqueous environment. Amongst various natural materials used for nanoparticle construction, plants seem to be the best candidates, and nanoparticles produced by plants are more stable, are of various sizes and shapes, and the rate of production is faster than in the case of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application

Padil¹,

Černík²

2013

IJN

212

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Background:Copper oxide (CuO) nanoparticles have attracted huge attention due to catalytic, electric, optical, photonic, textile, nanofluid, and antibacterial activity depending on the size, shape, and neighboring medium. In the present paper, we synthesized CuO nanoparticles using gum karaya, a natural nontoxic hydrocolloid, by green technology and explored its potential antibacterial application. Methods: The CuO nanoparticles were synthesized by a colloid-thermal synthesis process. The mixture contained various concentrations of CuCl 2 ⋅ 2H 2 O (1 mM, 2 mM, and 3 mM) and gum karaya (10 mg/mL) and was kept at 75°C at 250 rpm for 1 hour in an orbital shaker. The synthesized CuO was purified and dried to obtain different sizes of the CuO nanoparticles. The well diffusion method was used to study the antibacterial activity of the synthesized CuO nanoparticles. The zone of inhibition, minimum inhibitory concentration, and minimum bactericidal concentration were determined by the broth microdilution method recommended by the Clinical and Laboratory Standards Institute. Results: Scanning electron microscopy analysis showed CuO nanoparticles evenly distributed on the surface of the gum matrix. X-ray diffraction of the synthesized nanoparticles indicates the formation of single-phase CuO with a monoclinic structure. The Fourier transform infrared spectroscopy peak at 525 cm −1 should be a stretching of CuO, which matches up to the B 2u mode.The peaks at 525 cm −1 and 580 cm −1 indicated the formation of CuO nanostructure. Transmission electron microscope analyses revealed CuO nanoparticles of 4.8 ± 1.6 nm, 5.5 ± 2.5 nm, and 7.8 ± 2.3 nm sizes were synthesized with various concentrations of CuCl 2 ⋅ 2H 2 O (1 mM, 2 mM, and 3 mM). X-ray photoelectron spectroscopy profiles indicated that the O 1s and Cu 2p peak corresponding to the CuO nanoparticles were observed. The antibacterial activity of the synthesized nanoparticles was tested against Gram-negative and positive cultures. Conclusion:The formed CuO nanoparticles are small in size (4.8 ± 1.6 nm), highly stable, and have significant antibacterial action on both the Gram classes of bacteria compared to larger sizes of synthesized CuO (7.8 ± 2.3 nm) nanoparticles. The smaller size of the CuO nanoparticles (4.8 ± 1.6 nm) was found to be yielding a maximum zone of inhibition compared to the larger size of synthesized CuO nanoparticles (7.8 ± 2.3 nm). The results also indicate that increase in precursor concentration enhances an increase in particle size, as well as the morphology of synthesized CuO nanoparticles.

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“…Diffraction dots and diffuse rings of different lattice planes in the SAED pattern is in good accord with XRD results and the monoclinic system. Compared to the previous report on using liquid hydrolysis method to obtain spherical CuO nanoparticles, 32 in the US liquid hydrolysis method ultrasonic irradiation has great influence on obtaining rod-like CuO nanostructures. Furthermore, the surface uniformity of the rod-like nanostructures is achieved by using ultrasonic waves in the US liquid hydrolysis method.…”

Section: Resultsmentioning

confidence: 83%

Effect of Ultrasonic‐assisted Preparation Methods on Structure, Morphology and Optical Properties of Nanosized Cupric Oxide

Gharagozlou¹,

Rahnama²

2011

J Chinese Chemical Soc

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Cupric oxide is a p-type semiconductor with a narrow band-gap which is suitable for catalysis, electrochemical cells, field emission devices and gas sensor applications. Despite considerable efforts devoted to the preparation of the nanosized CuO, there is a lack of information about ultrasonic-assisted (US) preparation methods. Nanosized cupric oxide was successfully prepared through different ultrasonic-assisted (US) preparation methods. Furthermore, the influence of preparation method on the structure, morphology and optical properties of nanosized CuO has been reported. XRD patterns were identical to the single-phase pure CuO with a monoclinic structure. The enhancement of the crystallinity and crystallite size was observed for the sample prepared through the US thermal decomposition. The absorption band of CuO nanocrystals prepared through the US liquid hydrolysis shows a clear red shift of about 40 nm compared to those obtained with other preparation methods. Our results indicated that almost spherical CuO nanoparticles with an average size of 65 nm were prepared during the US thermal decomposition, while CuO rod-like nanostructures with an average diameter of about 16 nm were obtained via the US liquid hydrolysis method. The band gap values of nanosized CuO samples were larger than the reported value for the bulk CuO. Synthesized CuO samples by US methods with adjustable and controllable properties make the applicability of cupric oxide even more versatile.

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Highly dispersed CuO nanoparticles prepared by a novel quick-precipitation method

Cited by 269 publications

References 12 publications

Activation of Erk and p53 regulates copper oxide nanoparticle-induced cytotoxicity in keratinocytes and fibroblasts

Activation of Erk and p53 regulates copper oxide nanoparticle-induced cytotoxicity in keratinocytes and fibroblasts

Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application

Effect of Ultrasonic‐assisted Preparation Methods on Structure, Morphology and Optical Properties of Nanosized Cupric Oxide

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