Highly controlled robotic customized gel functionalization on 3D printed PCL framework for bone tissue engineering

Gupta, Deepak; Bellare, Jayesh

doi:10.1016/j.bprint.2021.e00175

Cited by 7 publications

(3 citation statements)

References 80 publications

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“…This methodology ensured the complete fusion of the two materials at the interface as well as the penetration of PCL inside the gel micropores. Further, at I 2 , crosslinking between −COOH of the etched PCL and −NH 2 of the gel led to the deposition of a covalently bonded gel layer over the PCL surface without any repulsion or introduction of unwanted gaps [79,81]. Therefore, the three layers bonded strongly with each other thus making a single piece of scaffold possessing multiple functionalities that includes high bioactivity, multi-scale porosity, surface functional groups, high mechanical strength, and controlled degradation rate.…”

Section: Discussionmentioning

confidence: 99%

Natural bone inspired core–shell triple-layered gel/PCL/gel 3D printed scaffolds for bone tissue engineering

Gupta,

Singh,

Bellare

2023

Biomed. Mater.

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Despite technological advancements in bone tissue engineering, it is still a challenge to fabricate a scaffold with high bioactivity as well as high mechanical strength that can promote osteogenesis as well as bear load. Here we developed a 3D printed gel-polymer multi-layered hybrid scaffold. The innermost layer is porous gel-based framework made of gelatin/carboxymethyl-chitin/nano-hydroxyapatite and is cryogenically 3D printed. Further, the second and middle layer of micro-engineered polycaprolactone (PCL) is infused in the gel with controlled penetration and tuneable coating thickness. The PCL surface is further coated with a third and final thin layer of gel matrix used for the first layer. This triple-layered structure demonstrates >3000% and >700% increase in compression strength and modulus post 8 weeks degradation, respectively, 83% reduction in degradation after 12 weeks, and 81% reduction in swelling as compared to only gel scaffolds. Further, nearly 300%, 250%, 50%, and 440% increase in cellular attachment, proliferation, protein generation, and mineralization, respectively are achieved as compared to only PCL scaffolds. Thus, these hybrid scaffolds offer high mechanical strength, slow degradation rate, high bioactivity, and high osteoconductivity. These multifunctional scaffolds have potential for reconstructing non-load-bearing bone defects like sinus lift, jaw cysts, and moderate load-bearing like reconstructing hard palate, orbital palate, and other craniomaxillofacial bone defects.

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Section: Discussionmentioning

confidence: 99%

Natural bone inspired core–shell triple-layered gel/PCL/gel 3D printed scaffolds for bone tissue engineering

Gupta,

Singh,

Bellare

2023

Biomed. Mater.

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“…(B) (a) PCL scaffold 3D printed and then coated with gel. (b) A 3D printed rabbit tibia model with segmental bone deficiency implanted with a PCL 3D printed scaffold that shows the potential applicability for bone scaffold development of patient 82 . (C) The performance of the in vivo 3D‐printed conduits.…”

Section: Bioprinting Applicationsmentioning

confidence: 99%

Viral and non‐viral gene therapy using 3D (bio)printing

Jahangiri

Rahimnejad

Boroujeni

et al. 2022

The Journal of Gene Medicine

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The overall success in launching discovered drugs is tightly restricted to the high rate of late‐stage failures, which ultimately inhibits the distribution of medicines in markets. As a result, it is imperative that methods reliably predict the effectiveness and, more critically, the toxicity of medicine early in the drug development process before clinical trials be continuously innovated. We must stay up to date with the fast appearance of new infections and diseases by rapidly developing the requisite vaccinations and medicines. Modern in vitro models of disease may be used as an alternative to traditional disease models, and advanced technology can be used for the creation of pharmaceuticals as well as cells, drugs, and gene delivery systems to expedite the drug discovery procedure. Furthermore, in vitro models that mimic the spatial and chemical characteristics of native tissues, such as a 3D bioprinting system or other technologies, have proven to be more effective for drug screening than traditional 2D models. Viral and non‐viral gene delivery vectors are a hopeful tool for combinatorial gene therapy, suggesting a quick way of simultaneously deliver multiple genes. A 3D bioprinting system embraces an excellent potential for gene delivery into the different cells or tissues for different diseases, in tissue engineering and regeneration medicine, in which the precise nucleic acid is located in the 3D printed tissues and scaffolds. Non‐viral nanocarriers, in combination with 3D printed scaffolds, are applied to their delivery of genes and controlled release properties. There remains, however, a big obstacle in reaching the full potential of 3D models because of a lack of in vitro manufacturing of live tissues. Bioprinting advancements have made it possible to create biomimetic constructions that may be used in various drug discovery research applications. 3D bioprinting also benefits vaccinations, medicines, and relevant delivery methods because of its flexibility and adaptability. This review discusses the potential of 3D bioprinting technologies for pharmaceutical studies.

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“…Macromolecules composed of numerous repeating subunits are called polymers, which are similar to biomaterials, such as hydrogels [ 1 ], silicone elastomers [ 2 ], and polycaprolactone (PCL) [ 3 ], and play a key role in applications with biological interfaces, including soft robotics [ 4 , 5 ], flexible electronics [ 6 ], and biomedical engineering.…”

Section: Introductionmentioning

confidence: 99%

3D Printing Soft Matters and Applications: A Review

Zhan

Guo

Cao

et al. 2022

IJMS

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The evolution of nature created delicate structures and organisms. With the advancement of technology, especially the rise of additive manufacturing, bionics has gradually become a popular research field. Recently, researchers have concentrated on soft robotics, which can mimic the complex movements of animals by allowing continuous and often responsive local deformations. These properties give soft robots advantages in terms of integration and control with human tissue. The rise of additive manufacturing technologies and soft matters makes the fabrication of soft robots with complex functions such as bending, twisting, intricate 3D motion, grasping, and stretching possible. In this paper, the advantages and disadvantages of the additive manufacturing process, including fused deposition modeling, direct ink writing, inkjet printing, stereolithography, and selective laser sintering, are discussed. The applications of 3D printed soft matter in bionics, soft robotics, flexible electronics, and biomedical engineering are reviewed.

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Highly controlled robotic customized gel functionalization on 3D printed PCL framework for bone tissue engineering

Cited by 7 publications

References 80 publications

Natural bone inspired core–shell triple-layered gel/PCL/gel 3D printed scaffolds for bone tissue engineering

Natural bone inspired core–shell triple-layered gel/PCL/gel 3D printed scaffolds for bone tissue engineering

Viral and non‐viral gene therapy using 3D (bio)printing

3D Printing Soft Matters and Applications: A Review

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