Highly conserved B-subunit genes of Shiga-like toxin II variants found in Escherichia coli O157 strains

Rüssmann, H

doi:10.1016/0378-1097(94)90525-8

Cited by 21 publications

(17 citation statements)

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“…Several variants of Stx2 have been found in STEC; in STEC O157, many of the strains carry Stx2, Stx2c, or both (27). Many previous studies have detected only the generic stx 2 gene (called generalstx 2 in this paper) and do not distinguish between variants of this gene.…”

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confidence: 75%

Multivariate Analyses Revealed Distinctive Features Differentiating Human and Cattle Isolates of Shiga Toxin-Producing Escherichia coli O157 in Japan

et al. 2011

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Genotypes of Shiga toxin-producing Escherichia coli (STEC) O157 isolated from humans and cattle were analyzed by uni-and multivariable logistic regression, and population structure methods, to gain insight into transmission and the nature of human infection. Eleven genotyping assays, including PCR typing of five virulence factors (stx 1 , stx 2 , stx 2c , eae, and ehxA) and a lineage-specific polymorphism assay using six markers (LSPA6), were considered in the analyses. The prevalence of the stx 1 , stx 2 , and stx 2c virulence factors was significantly different between human and cattle isolates. However, multivariable regression revealed that the presence of only the stx 2 gene was significantly associated with human isolates after controlling for confounding effects. LSPA6 typing demonstrated an apparent difference in the distribution of LSPA6 lineages between human and cattle isolates and a strong association between stx genotypes and LSPA6 genotypes. Population genetics tools identified three genetically distinct clusters of STEC O157. Each cluster was characterized by stx genotypes and LSPA6 genotypes. The human isolates typically comprised LSPA6 lineage I with stx 1 stx 2 strains and LSPA6 lineage I/II with stx 2 or stx 2 stx 2c strains. In contrast, the cattle isolates comprised LSPA6 lineage II strains with stx 2c or stx 2 stx 2c strains in addition to the clusters identified for the human isolates. Our analyses provide new evidence that the stx 2 gene is the most distinctive feature in human isolates compared to cattle isolates in Japan, and only a subset of the genetically diverse population isolated from cattle is involved in human illnesses. Our results may contribute to international comparisons and risk assessments of STEC O157.

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confidence: 75%

Multivariate Analyses Revealed Distinctive Features Differentiating Human and Cattle Isolates of Shiga Toxin-Producing Escherichia coli O157 in Japan

et al. 2011

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“…EHEC strains with stx 2 genes are significantly more frequently associated with HUS and other severe diseases than isolates with an stx 1 gene, which are more often associated with uncomplicated diarrhea or healthy individuals (6,36). Stx2 is closely related to a family of Stx2 variants or alleles, which includes Stx2c (48), Stx2d (36), Stx2e (56), and Stx2f (47), although Stx2c and Stx2d are produced by STEC strains isolated from humans (36,38,43,44,48,50). Additional genetic variants of the stx 2 gene have been described (5,14,28,55).…”

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confidence: 99%

Analysis of the Clonal Relationship of Shiga Toxin-Producing Escherichia coli Serogroup O165:H25 Isolated from Cattle

Geue

Selhorst

Schnick

et al. 2006

Appl Environ Microbiol

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“…To determine the presence of TP53 gene mutation, the double-stranded polymerase chain reaction products, which showed abnormalities by single-strand conformation polymorphism analysis, were subjected to cycle sequencing according to the method of Russmann et al 15 A total of I fig of double-stranded, polymerase chain reaction product, 8 flL terminator premix, and 3.2 pmol of primers were mixed in a reaction tube, to a final volume of 20 fL. The tubes were placed in a thermal cycler, reheated to 96~ and subjected to 25 cycles, each consisting of 10 seconds at 96~ 5 seconds at 50~ and 4 minutes at 60~ The cycle sequencing products were extracted with phenol/chloroform and ethanol-precipitated.…”

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confidence: 99%

Alterations of theTP53 gene and TP53 protein expression in epithelial ovarian cancer before and after chemotherapy

et al. 1998

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Background:A role for the TP53 (alias p53)tumor-suppressor gene in chemoresistance has recently been discussed, but little is known about the clinical relevance of the TP53 gene to chemoresistance. To elucidate the relevance of TP53 status to chemoresistance, we investigated the TP53 gene and TP53 protein expression in tumors from the same patients, before and after chemotherapy. Methods:Twenty-one patients with ovarian cancer, who had residual disease after primary surgery, were studied. These patients received chemotherapy consisting of cisplatin, doxorubicin, and cyclophosphamide, and then underwent a second surgery. Polymerase chain reaction-single strand conformation polymorphism analysis and cycle sequencing were performed to determine TP53 mutation. TP53 protein was detected by Western blot analysis. Results: Of the 21 patients studied, 9 responded to chemotherapy. Mutation of the TP53 gene was seen in 7 patients (2 responders and 5 nonresponders) before chemotherapy. After chemotherapy, another mutation of the gene was observed in 5 patients, all of whom were nonresponders. TP53 protein was detected in 10 patients (3 responders and 7 nonresponders) before chemotherapy. After chemotherapy, the expression of TP53 protein increased in these 3 nonresponders, and became positive in 2 other nonresponders. Conclusions: This study showed for the first time in clinical investigation that alterations to TP53 could develop in association with chemotherapy, and that TP53 status may relate to the mechanisms of chemoresistance in patients with epithelial ovarian cancer.Int J Clin Onco11998;3:240-246

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Highly conserved B-subunit genes of Shiga-like toxin II variants found in Escherichia coli O157 strains

Cited by 21 publications

References 20 publications

Multivariate Analyses Revealed Distinctive Features Differentiating Human and Cattle Isolates of Shiga Toxin-Producing Escherichia coli O157 in Japan

Multivariate Analyses Revealed Distinctive Features Differentiating Human and Cattle Isolates of Shiga Toxin-Producing Escherichia coli O157 in Japan

Analysis of the Clonal Relationship of Shiga Toxin-Producing Escherichia coli Serogroup O165:H25 Isolated from Cattle

Alterations of theTP53 gene and TP53 protein expression in epithelial ovarian cancer before and after chemotherapy

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