Highlights of the 3rd International Conference on High Pressure Bioscience and Biotechnology

Mignaco, Julio A.; Lima, L.M.T.R.; Rosenthal, Amauri; Foguel, Débora; Silva, Jerson L.

doi:10.1590/s0100-879x2005000800001

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…The first studies on the effects of pressure on biological systems originated more than 100 years ago. However, until the twentieth century, the effects of pressure on living matter were underestimated or unknown because of the severe technical limitations imposed by the lack of materials and technology that could sustain pressures ranging from a few to 100 MPa Mignaco et al 2005). However, until the twentieth century, the effects of pressure on living matter were underestimated or unknown because of the severe technical limitations imposed by the lack of materials and technology that could sustain pressures ranging from a few to 100 MPa Mignaco et al 2005).…”

Section: Summary and Concluding Remarksmentioning

confidence: 99%

High Pressure Bioscience

Akasaka¹,

Matsuki²

2015

Subcellular Biochemistry

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The book series SUBCELLULAR BIOCHEMISTRY is a renowned and well recognized forum for disseminating advances of emerging topics in Cell Biology and related subjects. All volumes are edited by established scientists and the individual chapters are written by experts on the relevant topic. The individual chapters of each volume are fully citable and indexed in Medline/Pubmed to ensure maximum visibility of the work.

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Section: Summary and Concluding Remarksmentioning

confidence: 99%

High Pressure Bioscience

Akasaka¹,

Matsuki²

2015

Subcellular Biochemistry

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Pressure-Inactivated Virus: A Promising Alternative for Vaccine Production

Silva

Barroso

Mendes

et al. 2015

Subcellular Biochemistry

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In recent years, many applications in diverse scientific fields with various purposes have examined pressure as a thermodynamic parameter. Pressure studies on viruses have direct biotechnological applications. Currently, most studies that involve viral inactivation by HHP are found in the area of food engineering and focus on the inactivation of foodborne viruses. Nevertheless, studies of viral inactivation for other purposes have also been conducted. HHP has been shown to be efficient in the inactivation of many viruses of clinical importance and the use of HHP approach has been proposed for the development of animal and human vaccines. Several studies have demonstrated that pressure can result in virus inactivation while preserving immunogenic properties. Viruses contain several components that can be susceptible to the effects of pressure. HHP has been a valuable tool for assessing viral structure function relationships because the viral structure is highly dependent on protein-protein interactions. In the case of small icosahedral viruses, incremental increases in pressure produce a progressive decrease in the folding structure when moving from assembled capsids to ribonucleoprotein intermediates (in RNA viruses), free dissociated units (dimers and/or monomers) and denatured monomers. High pressure inactivates enveloped viruses by trapping their particles in a fusion-like intermediate state. The fusogenic state, which is characterized by a smaller viral volume, is the final conformation promoted by HHP, in contrast with the metastable native state, which is characterized by a larger volume. The combined effects of high pressure with other factors, such as low or subzero temperature, pH and agents in sub-denaturing conditions (urea), have been a formidable tool in the assessment of the component's structure, as well as pathogen inactivation. HHP is a technology for the production of inactivated vaccines that are free of chemicals, safe and capable of inducing strong humoral and cellular immune responses. Here we present a current overview about the pressure-induced viral inactivation and the production of inactivated viral vaccines.

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