Highest Diploid Number Among Gymnotiformes: First Cytogenetic Insights into <i>Rhabdolichops</i> (Sternopygidae)

Suárez, Pablo; Barroso, Inae Cristina Guerreiro Pinto; Silva, Danillo dos Santos; Milhomem, Susana Suely Rodrigues; Cabral-de-Mello, Diogo Cavalcanti; Martins, Cesar; Pieczarka, Júlio César; Nagamachi, Cleusa Yoshiko

doi:10.1089/zeb.2016.1405

Cited by 10 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…D. cuneata represents an exception, given the increase in karyotype by two chromosomes with respect to the predicted ancestral karyotype of Pleuronectiformes. Chromosome fission is a plausible way for this diploid number to have been reached in D. cuneata , as has already been proposed for fish species of the genus Rhabdolichops of the Gymnotiformes order [ 42 ].…”

Section: Discussionmentioning

confidence: 91%

Evidence for a Robertsonian fusion in Solea senegalensis (Kaup, 1858) revealed by zoo-FISH and comparative genome analysis

et al. 2018

View full text Add to dashboard Cite

BackgroundSolea senegalensis (Kaup, 1858) is a commercially important flatfish species, belonging to the Pleuronectiformes order. The taxonomy of this group has long been controversial, and the karyotype of the order presents a high degree of variability in diploid number, derived from chromosomal rearrangements such as Robertsonian fusions. Previously it has been proposed that the large metacentric chromosome of S. senegalensis arises from this kind of chromosome rearrangement and that this is a proto-sex chromosome.ResultsIn this work, the Robertsonian origin of the large metacentric chromosome of S. senegalensis has been tested by the Zoo-FISH technique applied to two species of the Soleidae family (Dicologlossa cuneata and Dagetichthys lusitanica), and by comparative genome analysis with Cynoglossus semilaevis. From the karyotypic analysis we were able to determine a chromosome complement comprising 2n = 50 (FN = 54) in D. cuneata and 2n = 42 (FN = 50) in D. lusitanica. The large metacentric painting probe gave consistent signals in four acrocentric chromosomes of the two Soleidae species; and the genome analysis proved a common origin with four chromosome pairs of C. semilaevis. As a result of the genomic analysis, up to 61 genes were annotated within the thirteen Bacterial Artificial Chromosome clones analysed.ConclusionsThese results confirm that the large metacentric chromosome of S. senegalensis originated from a Robertsonian fusion and provide new data about the chromosome evolution of S. senegalensis in particular, and of Pleuronectiformes in general.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5216-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 91%

Evidence for a Robertsonian fusion in Solea senegalensis (Kaup, 1858) revealed by zoo-FISH and comparative genome analysis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…FISH probes for 18S and 5S rDNA were amplified by polymerase chain reaction (PCR) using genomic DNA from C. johanna , and the following primer pairs: 18S rDNA (18SF-5′ CCG CTT TGG TGA CTC TTG AT and 18SR-5′ CCG AGG ACC TCA CTA AAC CA) of Gross et al (2010); and 5S rDNA (5SF-5′ GCC ACA CCA CCC CTG AAC AC and 5SR-5′ GCC TAC GAC ACC TGG TAT TC) of Suarez et al (2017). In each PCR reaction were used: 100 ng/μl genomic DNA, 2,5 μl of 10× reaction buffer, 0.75 MgCl, 2,0 μl of DNTP mix (2 mM), 1 μl of each primer (10 mM), 1U of enzyme Taq Polymerase (Invitrogen) and 16.5 μl of pure water.…”

Section: Methodsmentioning

confidence: 99%

Karyoevolution ofCrenicichlaheckel 1840 (Cichlidae, Perciformes): a process mediated by inversions

et al. 2019

Self Cite

View full text Add to dashboard Cite

Crenicichla (Cichliformes, Cichlidae) present a highly conserved diploid number 2n=48 with fundamental numbers varying between 52 and 62. We analyzed four species in order to investigate the role of repetitive DNA in chromosome evolution in the genus. Crenicichla johanna , Crenicichla cf. saxatilis and Crenicichla cf. regani have 2n=48 (8 m/sm and 40st/a) and FN=56, while Crenicichla sp. ‘Xingu I’ has 2n=48 (48 st/a) and FN=48. Different patterns of constitutive heterochromatin distribution were observed including pericentric, interstitial and whole arm C bands. A single chromosome bears 18S rDNA clusters in most species, except C. johanna , where population variation exists in terms of the quantity and distribution of clusters and their association with interstitial telomeric sequences. All species showed hybridization of 5S rDNA sequences in an interstitial region on an acrocentric chromosome pair. The karyotypic differences and maintenance of the diploid number supports chromosome evolution mediated by inversions in Crenicichla . The telomeric and 18S rDNA sequence association in various chromosomes of C. johanna are proposed to represent hotspots for breakage, favoring intra-chromosomal rearrangements. The results suggest that repetitive sequences can contribute to microstructural cytogenetic diversity in Crenicichla .

show abstract

“…Cytogenetics has been an important tool in cytotaxonomy and has proved to be very useful in understanding the evolutionary processes behind the diversification of Gymnotus . The Gymnotiformes order has considerable variation, not only in diploid number (from 2 n = 24 in Apteronotus albifrons , Howell, 1972 ; Almeida-Toledo et al, 1981 ; Mendes et al, 2012 ; to 2 n = 74 in Rhabdolichops cf eastward , Suárez et al, 2017 ) but also in the karyotype formula and location of repetitive sequences (Fernandes et al, 2005 ; Almeida-Toledo et al, 2007 ; Silva et al, 2009 ; da Silva et al, 2013 ; Jesus et al, 2016 ; Araya-Jaime et al, 2017 ; Batista et al, 2017 ; Sousa et al, 2017 ; Takagui et al, 2017 ). Recently, fluorescence in situ hybridization (FISH), has played an important role in understanding the genome structure of fish species (Yi et al, 2003 ; Cabral-de-Mello and Martins, 2010 ; Martins et al, 2011 ; Vicari et al, 2011 ; Gornung, 2013 ; Knytl et al, 2013 ; Yano et al, 2017 ) and molecular cytogenetic studies in Gymnotiformes have shown dynamic reorganization, including pericentric inversions observed through repetitive DNA position (Fernandes et al, 2017 ), sequence dispersion via transposable elements and the association between different repetitive sequences (Utsunomia et al, 2014 ; da Silva et al, 2016 ; Machado et al, 2017 ) and the presence of different sex chromosome systems (Margarido et al, 2007 ; Henning et al, 2008 , 2011 ; da Silva et al, 2011 , 2014 ; Almeida et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Extensive Karyotype Reorganization in the Fish Gymnotus arapaima (Gymnotiformes, Gymnotidae) Highlighted by Zoo-FISH Analysis

et al. 2018

View full text Add to dashboard Cite

The genus Gymnotus (Gymnotiformes) contains over 40 species of freshwater electric fishes exhibiting a wide distribution throughout Central and South America, and being particularly prevalent in the Amazon basin. Cytogenetics has been an important tool in the cytotaxonomy and elucidation of evolutionary processes in this genus, including the unraveling the variety of diploid chromosome number (2n = from 34 to 54), the high karyotype diversity among species with a shared diploid number, different sex chromosome systems, and variation in the distribution of several Repetitive DNAs and colocation and association between those sequences. Recently whole chromosome painting (WCP) has been used for tracking the chromosomal evolution of the genus, showing highly reorganized karyotypes and the conserved synteny of the NOR bearing par within the clade G. carapo. In this study, painting probes derived from the chromosomes of G. carapo (GCA, 2n = 42, 30 m/sm + 12 st/a) were hybridized to the mitotic metaphases of G. arapaima (GAR, 2n = 44, 24 m/sm + 20 st/a). Our results uncovered chromosomal rearrangements and a high number of repetitive DNA regions. From the 12 chromosome pairs of G. carapo that can be individually differentiated (GCA1–3, 6, 7, 9, 14, 16, and 18–21), six pairs (GCA 1, 9, 14, 18, 20, 21) show conserved homology with GAR, five pairs (GCA 1, 9, 14, 20, 21) are also shared with cryptic species G. carapo 2n = 40 (34 m/sm + 6 st/a) and only the NOR bearing pair (GCA 20) is shared with G. capanema (GCP 2n = 34, 20 m/sm + 14 st/a). The remaining chromosomes are reorganized in the karyotype of GAR. Despite the close phylogenetic relationships of these species, our chromosome painting studies demonstrate an extensive reorganization of their karyotypes.

show abstract

Highest Diploid Number Among Gymnotiformes: First Cytogenetic Insights into Rhabdolichops (Sternopygidae)

Cited by 10 publications

References 45 publications

Evidence for a Robertsonian fusion in Solea senegalensis (Kaup, 1858) revealed by zoo-FISH and comparative genome analysis

Evidence for a Robertsonian fusion in Solea senegalensis (Kaup, 1858) revealed by zoo-FISH and comparative genome analysis

Karyoevolution ofCrenicichlaheckel 1840 (Cichlidae, Perciformes): a process mediated by inversions

Extensive Karyotype Reorganization in the Fish Gymnotus arapaima (Gymnotiformes, Gymnotidae) Highlighted by Zoo-FISH Analysis

Contact Info

Product

Resources

About