Higher sensitivity to Cu2+ exposure of Microcystis aeruginosa in late lag phase is beneficial to its control

Xian, Xuanxuan; Li, Xi; Ye, Chengsong; Wan, Kun; Feng, Mingbao; Luo, Chen; Yu, Xin

doi:10.1016/j.watres.2022.118207

Cited by 27 publications

(21 citation statements)

References 60 publications

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“…Existing automatic monitoring equipment for water quality could achieve the long-term and real-time monitoring of key indicators such as DO, pH, and chlorophyll-a, and we have also demonstrated the feasibility of early warning through these indicators in our previous work. In the late lag phase, the daily average ΔDO significantly increased . Some unusual changes may be an important reminder that effective intervening measures should be done to control cyanobacterial bloom in source waters, such as the use of algicide (e.g., chlorine).…”

Section: Resultsmentioning

confidence: 99%

“…The growth and expansion culture of M. aeruginosa was in the BG-11 medium (Hopebio, China). Culture conditions were described in our previous study …”

Section: Methodsmentioning

confidence: 99%

“…The cell density in the late lag phase was the result of the gradual increase of M. aeruginosa cells. For exponential phase samples, cell density was diluted in situ to be the same as that in the late lag phase to eliminate its influence. , Cultures of M. aeruginosa in the exponential phase were centrifuged, and the supernatant was retained; then, portions of the centrifuged cells were resuspended in the supernatant according to the required concentration . The composition and concentration of extracellular substances (e.g., DOC and microcystin) were not affected during this process.…”

Section: Methodsmentioning

confidence: 99%

“…Culture conditions were described in our previous study. 28 2.2. Preparing M. aeruginosa Samples in Late Lag and Exponential Phases.…”

Section: Cyanobacterial Culturementioning

confidence: 99%

“…At this time, cyanobacteria have a strong photosynthesis process, and they are more sensitive to the adverse external environment. In a previous work, we proved that a lower dose of CuSO 4 effectively inactivated cyanobacterial cells in the late lag phase (late delay stage), and the inactivation mechanism was mainly due to its higher sensitivity to CuSO 4 compared with that in the exponential phase (development stage) . Therefore, investigating the inactivation of chlorination on cyanobacteria at the late delayed stage would contribute to cyanobacterial control with a high efficiency and a low consumption of chlorine.…”

Section: Introductionmentioning

confidence: 99%

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Chlorination for Microcystis aeruginosa in the Late Lag Phase: Higher Inactivation Efficiency and Lower Environmental Risk

Xian

Wei

et al. 2023

ACS EST Water

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Chlorine is often employed to dispose cyanobacteria-laden waters for inactivating cells or improving its removal efficiency. However, previous studies mostly focused on cyanobacterial blooms occurring in the exponential or stationary phase, and thus, cyanobacterial characteristics in the unblooming stage (lag phase) and their influence on chlorination were not well understood. Herein, Microcystis aeruginosa in late lag and exponential phases with the same cell density were selected to simulate unblooming and blooming stages, respectively, and their response to chlorination was investigated. Compared with the exponential phase, results showed that cyanobacteria in the late lag phase consumed lower chlorine but produced higher intracellular reactive oxygen species (ROS). Then, higher rate constants of photosynthetic activity inactivation (k f ) and membrane damage (k m ) were found in the late lag phase. Chlorine of >1.0 mg L −1 could degrade microcystin in this phase, and microcystin could be oxidized to below 1.0 μg L −1 by 2.0 mg L −1 chlorine. Besides, lower extracellular dissolved organic carbon (DOC), UV absorbance at 254 nm (UV 254 ), and their variation range were also observed in the late lag phase. These findings indicated that the chlorination performance in the two phases is different, and thus, it is necessary to reduce the applied dosage of chlorine to treat cyanobacterialaden source waters in the (late) lag phase.

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Section: Resultsmentioning

confidence: 99%

“…The growth and expansion culture of M. aeruginosa was in the BG-11 medium (Hopebio, China). Culture conditions were described in our previous study …”

Section: Methodsmentioning

confidence: 99%