Higher production of rabies virus in serum-free medium cell cultures on microcarriers

Frazzati-Gallina, Neuza M.; Paoli, Rosana L.; Mourão-Fuches, Regina Maria; Jorge, Soraia Attie Calil; Pereira, Carlos Augusto

doi:10.1016/s0168-1656(01)00362-5

Cited by 51 publications

(37 citation statements)

References 18 publications

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“…1E, G and I for MDBK, CER and CRIB cells, respectively. In fact, there are many advantages in using serum-free culture systems; first it has been suggested that cells cultured without serum/protein seems to be more favourable for viral infection than cultured by using the serum-supplemented medium; second bovine serum may contains viral inhibitors, such proteases, that decreases the virus stability; and third bovine serum may contains exogenous pathogens and also prions [7,13,14,15] .…”

Section: Resultsmentioning

confidence: 99%

“…3A and B). Afterwards, the importance of BoHV growth behaviour in a nonpermissive system has been mentioned as a viable alternative culture system, because it allows for the analysis of fine details from particular step where the growth is arrested [4,14] . In this aspect, it was also demonstrated the ability of CER cells support bovine respiratory syncytial virus infection [5] .…”

Section: Resultsmentioning

confidence: 99%

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Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

Cardoso¹,

Ferrari²,

Luvizotto³

et al. 2007

American J. of Biochemistry and Biotechnology

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Abstract:The detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5µg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in a statistic continuous culture system. The results obtained by CRIB and MDBK cells adaptation showed steady growth after 10 th passages in OPTI-PRO medium, 20 th passages in VP-SFM medium and 30 th passages in RPMI 1640 medium, respectively. The OPTI-PRO and RPMI 1640 media were not able to support CER growth, even supplemented by IGF-1 and present apoptotic cells at 72h post-infection. The CER cells seeded after adaptation by 10 th passages in VP-SFM medium added by 5µg/20ml of IGF-1 growth factor revealed the best virus titres compared to MDBK and CRIB cell lines. It was concluded that CER, MDBK and CRIB cells infected by BoHV-5 serotype could be used for laboratory diagnosis propagated on much safer culture system of high biotechnology advances.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

Cardoso¹,

Ferrari²,

Luvizotto³

et al. 2007

American J. of Biochemistry and Biotechnology

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“…MDCK cells grown in serum-free media was used for the production of Influenza H5N1 virus used as a vaccine [40]. Rabies virus used for vaccine production was grown to higher titers in Vero cells in serum free than in serum-containing cell growth media [41].…”

Section: Serum Vs Serum-free Cell Culture Mediamentioning

confidence: 99%

The Art of Animal Cell Culture for Virus Isolation

Lednicky¹,

Wyatt²

2012

Biomedical Tissue Culture

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“…The cashew has a long record as a useful plant, but only in the twentieth century did it become an important tree crop (6). It has the potential to provide source of livelihood for the cashew growers, empower rural women in the processing sector, create employment opportunities and generate foreign exchange through exports.…”

Section: Introductionmentioning

confidence: 99%

Investment in Cashew Kernel Oil Production: Cost and Return Analysis of Three Processing Methods

Yahaya¹,

Taiwo²,

Shittu³

et al. 2012

ECONOMICS

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Three methods of expressing cashew (Anacardium occidentale L) oil from kernel were investigated. The physicochemical characteristics of the resulting oils were assayed. Parameters characterized included refractive index, melting point, viscosity ratio, acid value, iodine number, peroxide value, saponification value etc. While there is no significant difference in the value for specific gravity, moisture content, refractive index, viscosity ratio, melting point; there are differences in value for chemical variables such as saponification value, iodine value and peroxide value. The highest yield value of 48.8% was recorded for soxhlet method of extraction. The high value of 10.12 meq/kg obtained for aqueous method can be rationalized on the basis that traces of water cannot be completely ruled out and this result to hydrolysis of the ester bond of the triglyceride. The Cost and return analysis of the three methods of expressing cashew kernel oil was also examined. The method of evaluation involved an investment decision model comprising Net present value (NPV), Benefit Cost Ratio (BCR) and Internal Rate of Return (IRR) quality parameters of the resulting oils were characterized. The investment profile revealed that all the methods were viable, however the soxhlet method has the highest NPV (N227, 727 at 21% and N328, 678 at 32%) and BCR thus indicating its high returns on investment.

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Higher production of rabies virus in serum-free medium cell cultures on microcarriers

Cited by 51 publications

References 18 publications

Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

The Art of Animal Cell Culture for Virus Isolation

Investment in Cashew Kernel Oil Production: Cost and Return Analysis of Three Processing Methods

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