Higher plant NADP+-dependent isocitrate dehydrogenases, ammonium assimilation and NADPH production

Hodges, Michael; Flesch, Valérie; Gálvez, Susana; Bismuth, E.

doi:10.1016/s0981-9428(03)00062-7

Cited by 122 publications

(84 citation statements)

References 44 publications

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“…Gln is not only the major AA used for N transport in NC, but also a key metabolite that acts as an amino donor to other free amino acids, primarily catalyzed by glutamate synthase. This pathway interacts with carbohydrate metabolism or the energy status of the plant leaves (Hodges et al 2003). Gln and Glu can be used to form Asp and Asn, and these four AA are used to translocate …”

Section: Resultsmentioning

confidence: 99%

The changes of contents of selected free amino acids associated with cadmium stress in Noccaea caerulescens and Arabidopsis halleri

Zemanová¹,

Pavlı́k²,

Pavlı́ková³

et al. 2013

Plant Soil Environ.

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Changes of free amino acid (AA) contents (glutamic acid, glutamine, aspartic acid, asparagines, proline, hydroxyproline) in Noccaea caerulescens and Arabidopsis halleri under cadmium soil contamination (Cd1 = 30, Cd2 = 60, Cd3 = 90 mg/kg soil) are reported. Results of the pot experiment confirmed different effect of Cd on N. caerulescens in contrast to A. halleri and the higher stress adaptation of A. halleri. Total free AA contents in both plant species were not significantly modified by Cd contamination. The glutamic acid and glutamate contents in plant biomass were decreased under Cd2 and Cd3 stress. The declines of contents of both AA can be caused by intensive syntheses of plant defense elicitors, but declines in A. halleri were significantly lower in contrast to N. caerulescens. The content of aspartic acid was increased in N. caerulescens under Cd stress, but in A. halleri its changes were not observed. The different pathways of nitrogen utilization of tested plants were confirmed: the major AA forms used for nitrogen transport are glutamate for N. caerulescens and asparagine for A. halleri. The increase of proline content was determined only in N. caerulescens growing under Cd stress in the beginning of growing period.

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Section: Resultsmentioning

confidence: 99%

The changes of contents of selected free amino acids associated with cadmium stress in Noccaea caerulescens and Arabidopsis halleri

Zemanová¹,

Pavlı́k²,

Pavlı́ková³

et al. 2013

Plant Soil Environ.

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“…+ to 2OG and NAD(P)H, with 2OG being transported into the chloroplast via a 2OG/ malate transporter (143).…”

Section: Lipid Synthesismentioning

confidence: 99%

Metabolic Control of Redox and Redox Control of Metabolism in Plants

Geigenberger

Fernie

2014

Antioxidants & Redox Signaling

139

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Significance: Reduction-oxidation (Redox) status operates as a major integrator of subcellular and extracellular metabolism and is simultaneously itself regulated by metabolic processes. Redox status not only dominates cellular metabolism due to the prominence of NAD(H) and NADP(H) couples in myriad metabolic reactions but also acts as an effective signal that informs the cell of the prevailing environmental conditions. After relay of this information, the cell is able to appropriately respond via a range of mechanisms, including directly affecting cellular functioning and reprogramming nuclear gene expression. Recent Advances: The facile accession of Arabidopsis knockout mutants alongside the adoption of broad-scale post-genomic approaches, which are able to provide transcriptomic-, proteomic-, and metabolomic-level information alongside traditional biochemical and emerging cell biological techniques, has dramatically advanced our understanding of redox status control. This review summarizes redox status control of metabolism and the metabolic control of redox status at both cellular and subcellular levels. Critical Issues: It is becoming apparent that plastid, mitochondria, and peroxisome functions influence a wide range of processes outside of the organelles themselves. While knowledge of the network of metabolic pathways and their intraorganellar redox status regulation has increased in the last years, little is known about the interorganellar redox signals coordinating these networks. A current challenge is, therefore, synthesizing our knowledge and planning experiments that tackle redox status regulation at both inter-and intracellular levels. Future Directions: Emerging tools are enabling ever-increasing spatiotemporal resolution of metabolism and imaging of redox status components. Broader application of these tools will likely greatly enhance our understanding of the interplay of redox status and metabolism as well as elucidating and characterizing signaling features thereof. We propose that such information will enable us to dissect the regulatory hierarchies that mediate the strict coupling of metabolism and redox status which, ultimately, determine plant growth and development.

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“…Several studies surveying metabolic changes of plants exposed to environmental or genetic perturbation have variously indicated important roles of 2-oxoglutarate in ammonium assimilation (Mü ller et al, 2001;Hodges, 2002;Hodges et al, 2003;Dutilleul et al, 2005;Fritz et al, 2006;Schneidereit et al, 2006;Noguchi and Yoshida, 2008), gibberellin biosynthesis (Hedden and Kamiya, 1997), and flavonoid and glucosinolate formation (Saito et al, 1999;Kliebenstein et al, 2001). However, as yet few studies have aimed at investigating the contribution of the 2-oxoglutarate dehydrogenase complex to the control of plant respiration.…”

mentioning

confidence: 99%

Inhibition of 2-Oxoglutarate Dehydrogenase in Potato Tuber Suggests the Enzyme Is Limiting for Respiration and Confirms Its Importance in Nitrogen Assimilation

et al. 2008

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The 2-oxoglutarate dehydrogenase complex constitutes a mitochondrially localized tricarboxylic acid cycle multienzyme system responsible for the conversion of 2-oxoglutarate to succinyl-coenzyme A concomitant with NAD + reduction. Although regulatory mechanisms of plant enzyme complexes have been characterized in vitro, little is known concerning their role in plant metabolism in situ. This issue has recently been addressed at the cellular level in nonplant systems via the use of specific phosphonate inhibitors of the enzyme. Here, we describe the application of these inhibitors for the functional analysis of the potato (Solanum tuberosum) tuber 2-oxoglutarate dehydrogenase complex. In vitro experiments revealed that succinyl phosphonate (SP) and a carboxy ethyl ester of SP are slow-binding inhibitors of the 2-oxoglutarate dehydrogenase complex, displaying greater inhibitory effects than a diethyl ester of SP, a phosphono ethyl ester of SP, or a triethyl ester of SP. Incubation of potato tuber slices with the inhibitors revealed that they were adequately taken up by the tissue and produced the anticipated effects on the in situ enzyme activity. In order to assess the metabolic consequences of the 2-oxoglutarate dehydrogenase complex inhibition, we evaluated the levels of a broad range of primary metabolites using an established gas chromatography-mass spectrometry method. We additionally analyzed the rate of respiration in both tuber discs and isolated mitochondria. Finally, we evaluated the metabolic fate of radiolabeled acetate, 2-oxoglutarate or glucose, and 13 C-labeled pyruvate and glutamate following incubation of tuber discs in the presence or absence of either SP or the carboxy ethyl ester of SP. The data obtained are discussed in the context of the roles of the 2-oxoglutarate dehydrogenase complex in respiration and carbon-nitrogen interactions.

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Higher plant NADP+-dependent isocitrate dehydrogenases, ammonium assimilation and NADPH production

Cited by 122 publications

References 44 publications

The changes of contents of selected free amino acids associated with cadmium stress in Noccaea caerulescens and Arabidopsis halleri

The changes of contents of selected free amino acids associated with cadmium stress in Noccaea caerulescens and Arabidopsis halleri

Metabolic Control of Redox and Redox Control of Metabolism in Plants

Inhibition of 2-Oxoglutarate Dehydrogenase in Potato Tuber Suggests the Enzyme Is Limiting for Respiration and Confirms Its Importance in Nitrogen Assimilation

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