Higher-plant mitochondrial ribosomes contain a 5S ribosomal ribonucleic acid component

Leaver, Christopher J.; Harmey, Matthew A.

doi:10.1042/bj1570275

Cited by 70 publications

(27 citation statements)

References 13 publications

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“…However, there is as yet no information to indicate whether higher-plant mitochondrial and cytosol 5 S RNAs are distinct molecular entities (indeed, the two species cannot be distinguished by electrophoresis in 10% polyacrylamide gels [5] C AC GC AAU GAC AGC AAAU' AAGC AGAAC AAAGC GAAAAC GU GAU AGU GGC GAAAU AG,G,AC GGU GGAU AG,G,AU AAG,G,U GGAAU GGGC GGGU AG,AG,G,U AG,G,, This table lists sequences of oligonucleotides in the numbered spots on the fingerprints shown in figs.3 and 4. Cytosol molar yields were determined directly by measurement of radioactivity in spots.…”

Section: Resultsmentioning

confidence: 99%

“…Plant mitoribosomes also contain a 5 S RNA component [5] but not, apparently, the '5.8'S RNA species which is hydrogen-bonded to the large (26 S) rRNA of plant cytoribosomes [6]. The sedimentation coefficient of higher-plant mitoribosomes is 77-78 S [2,3] , very similar to that of plant cytoribosomes (80 S), Nevertheless, protein synthesis in plant mitochondria is sensitive to many of the antibiotics which differentially inhibit protein synthesis in prokaryotes, as well as in chloroplasts and in mitochondria of other organisms…”

Section: Introductionmentioning

confidence: 99%

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Unique species of 5 S, 18 S, and 26 S ribosomal RNA in wheat mitochondria

et al. 1976

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unique species of 5 S, 18 S, and 26 S ribosomal RNA in wheat mitochondria

et al. 1976

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“…7A and 7B). The latter RNA species is a characteristic constituent of cytoplasmic ribosomes but not of bacterial ribosomes [20,21].…”

Section: Ribosomal Rna From Mitochondria Isolated Under Different Conmentioning

confidence: 99%

The 73 S ribosome of Neurospora crassa is the native mitochondrial ribosome

Michel

Hallermayer

Harmey

et al. 1977

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

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SummaryThe effect of the inclusion of EDTA and of heparin, in media used in the isolation of mitochondria, on the mitochindrial ribosome has been investigated.1. Mitochondria isolated from Neurospora crassa in the presence of EDTA contain only a single type of monomeric ribosome, viz. 73 S.2. Mitochondria isolated in the presence of Mg 2÷ contain both 79-S and 73-S monomeric ribosomes. The heterogeneity of the ribosomes was demonstrated by (a) ultracentrifugation on sucrose gradients, (b) electron microscopy, (c) immunoprecipitation with antibodies against mitochondrial 73-S and 79-S cytoplasmic ribosomes, (d) gel electrophoresis of high and low molecular weight RNAs.3. Inclusion of heparin in all media used for the isolation of mitochondria and ribosomes resulted in (a) dissociation of 73-S mitochondrial ribosomes into 50-S and 37-S subunits; (b) stabilization of 79-S cytoplasmic ribosomes; (c) in the case of mitochondria isolated in the presence of Mg 2÷ containing both 73-S and 79-S ribosomes, heparin causes the selective dissociation of the 73 S monosome to yield ribosomes containing only a single monomeric ribosome type, viz. 79 S.4. It is concluded that (a) the 79-S ribosomes present in mitochondria isolated in the presence of Mg 2÷ are contaminating cytoplasmic ribosomes, (b) the 73-S ribosomes are the real functional mitochondrial ribosomes of Neurospora crassa.

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“…Surprisingly, in view of its otherwise ubiquitous distribution, 5S rRNA appears not to be universally present in mitochondrial systems. Plant mitochondrial ribosomes do contain a distinctive 5S rRNA species (Cunningham et al 1976;Leaver and Harmey 1976;Spencer et al 1981), encoded by the mitochondrial genome (Bonen and Gray 1980;Oda et al 1992;Unseld et al 1997;Kubo et al 2000). A recognizable 5S rRNA gene is also present in some protist mitochondrial genomes, notably those of certain green, red, and brown algae (Wolff et al 1994;Ohta et al 1998;Burger et al 1999;Turmel et al 1999Turmel et al , 2002aTurmel et al , 2000bOudot-Le Secq et al 2001, 2002 and jakobid flagellates (Lang et al 1996.…”

Section: Introductionmentioning

confidence: 99%

Discovery and characterization of Acanthamoeba castellanii mitochondrial 5S rRNA

Bullerwell

Schnare²,

Gray³

2003

RNA

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Although 5S rRNA is a highly conserved and universal component of eubacterial, archaeal, chloroplast, and eukaryotic cytoplasmic ribosomes, a mitochondrial DNA-encoded 5S rRNA has so far been identified only in land plants and certain protists. This raises the question of whether 5S rRNA is actually required for and used in mitochondrial translation. In the protist Acanthamoeba castellanii, BLAST searches fail to reveal a 5S rRNA gene in the complete mitochondrial genome sequence, nor is a 5S-sized RNA species detectable in ethidium bromide-stained gels of highly purified mitochondrial RNA preparations. Here we show that an alternative visualization technique, UV shadowing, readily detects a novel, mitochondrion-specific small RNA in A. castellanii mitochondrial RNA preparations, and that this RNA species is, in fact, a 5S rRNA encoded by the A. castellanii mitochondrial genome. These results emphasize the need for caution when interpreting negative results that suggest the absence of 5S rRNA and/or a mitochondrial DNA-encoded 5S rRNA sequence in other (particularly protist) mitochondrial systems.

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Higher-plant mitochondrial ribosomes contain a 5S ribosomal ribonucleic acid component

Cited by 70 publications

References 13 publications

Unique species of 5 S, 18 S, and 26 S ribosomal RNA in wheat mitochondria

Unique species of 5 S, 18 S, and 26 S ribosomal RNA in wheat mitochondria

The 73 S ribosome of Neurospora crassa is the native mitochondrial ribosome

Discovery and characterization of Acanthamoeba castellanii mitochondrial 5S rRNA

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