Higher-order dielectrophoresis of nonspherical particles

Nili, Hossein; Green, Nicolas G.

doi:10.1103/physreve.89.063302

Cited by 28 publications

(23 citation statements)

References 25 publications

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“…Higher order electrophysical effects are described by DEP mobility (µ DEP ). It can be expressed as (Jones, 1995;Nili and Green, 2014;Hilton and Hayes, 2019):…”

Section: Theorymentioning

confidence: 99%

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Liu

Hayes

2020

Front. Microbiol.

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Salmonella is an important pathogen and is a worldwide threat to food safety and public health. Surveillance of serotypes and fundamental biological and biochemical studies are supported by a wide variety of established and emerging bioanalytical techniques. These include classic serotyping based on the Kauffmann-White nomenclature and the emerging whole genome sequencing strategy. Another emerging strategy is native whole cell biophysical characterization which has yet to be applied to Salmonella. However, this technique has been shown to provide high resolution differentiation of serotypes with several other paired strains of other microbes and pathogens. To demonstrate that biophysical characterization might be useful for Salmonella serotyping, the closely related strains sv. Cubana and sv. Poona were chosen for study. These two serovars were subjected to biophysical measurements on a dielectrophoresisbased microfluidic device that generated full differentiation of the unlabeled and native cells. They were differentiated by the ratio of electrophoretic (EP) to dielectrophoretic (DEP) mobilities. This differentiation factor is 2.7 ± 0.3 × 10 10 V/m 2 for sv. Cubana, versus 2.2 ± 0.3 × 10 10 V/m 2 for sv. Poona. This work shows for the first time the differentiation, concentration, and characterization of the Salmonella serotypes by exploiting their biophysical properties. It may lead to a less expensive and more decentralized new tool and method for microbiologists, complimenting and working in parallel with other characterization methods.

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“…Higher order electrophysical effects are described by DEP mobility (µ DEP ). It can be expressed as (Jones, 1995;Nili and Green, 2014;Hilton and Hayes, 2019):…”

Section: Theorymentioning

confidence: 99%

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Liu

Hayes

2020

Front. Microbiol.

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“…Dielectrophoresis is based on the action of an inhomogeneous electric field on a temporary induced dipole (or multipole 13 , 14 ). The dielectrophoretic force in an ac electric field depends on the volume of the particle, on its relative polarizability, and on the spatial change of the electric field.…”

Section: Introductionmentioning

confidence: 99%

Bridging the scales in high-throughput dielectrophoretic (bio-)particle separation in porous media

Pesch

Lorenz

Sachdev

et al. 2018

Sci Rep

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Dielectrophoresis (DEP) is a versatile technique for the solution of difficult (bio-)particle separation tasks based on size and material. Particle motion by DEP requires a highly inhomogeneous electric field. Thus, the throughput of classical DEP devices is limited by restrictions on the channel size to achieve large enough gradients. Here, we investigate dielectrophoretic filtration, in which channel size and separation performance are decoupled because particles are trapped at induced field maxima in a porous separation matrix. By simulating microfluidic model porous media, we derive design rules for DEP filters and verify them using model particles (polystyrene) and biological cells (S. cerevisiae, yeast). Further, we bridge the throughput gap by separating yeast in an alumina sponge and show that the design rules are equally applicable in real porous media at high throughput. While maintaining almost 100% efficiency, we process up to 9 mL min−1, several orders of magnitude more than most state-of-the-art DEP applications. Our microfluidic approach provides new insight into trapping dynamics in porous media, which even can be applied in real sponges. These results pave the way toward high-throughput retention, which is capable of solving existing problems such as cell separation in liquid biopsy or precious metal recovery.

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“…The accuracy of the EM method under various scenarios and using various orders of approximation was investigated, for example, in Refs. [14][15][16] and in Ref. [17].…”

Section: A State Of the Artmentioning

confidence: 95%

Control-oriented model of dielectrophoresis and electrorotation for arbitrarily shaped objects

Michálek¹,

Bolopion²,

Hurák³

et al. 2019

Phys. Rev. E

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The most popular modeling approach for dielectrophoresis (DEP) is the effective multipole (EM) method. It approximates the polarization-induced charge distribution in an object of interest by a set of multipolar moments. The Coulombic interaction of these moments with the external polarizing electric field then gives the DEP force and torque acting on the object. The multipolar moments for objects placed in arbitrary harmonic electric fields are, however, known only for spherical objects. This shape restriction significantly limits the use of the EM method. We present an approach for online (in real time) computation of multipolar moments for objects of arbitrary shapes having even arbitrary internal composition (inhomogeneous objects, more different materials, etc.). We exploit orthonormality of spherical harmonics to extract the multipolar moments from a numerical simulation of the polarized object. This can be done in advance (offline) for a set of external electric fields forming a basis so that the superposition principle can then be used for online operation. DEP force and torque can thus be computed in fractions of a second, which is needed, for example, in model-based control applications. We validate the proposed model against reference numerical solutions obtained using Maxwell stress tensor. We also analyze the importance of the higher-order multipolar moments using a sample case of a Tetris-shaped micro-object placed inside a quadrupolar microelectrode array and exposed to electrorotation. The implementation of the model in MATLAB and COMSOL is offered for free download.

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Higher-order dielectrophoresis of nonspherical particles

Cited by 28 publications

References 25 publications

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Bridging the scales in high-throughput dielectrophoretic (bio-)particle separation in porous media

Control-oriented model of dielectrophoresis and electrorotation for arbitrarily shaped objects

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