Higher numbers of memory B-cells and Th2-cytokine skewing in high responders to hepatitis B vaccination

Doedée, Anne; Kannegieter, Nynke M.; Öztürk, Kemal; Loveren, Henk Van; Janssen, Riny; Buisman, Anne Marie

doi:10.1016/j.vaccine.2015.12.027

Cited by 14 publications

(6 citation statements)

References 33 publications

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“…This is in line with the observed HPV-specific IFN-y responses. After the third vaccination no clear expansion of T cells was seen in both cohorts, similar to what was reported for antigen-specific T cells upon booster vaccination with a hepatitis B vaccine ( 39 ) and after Tdap vaccination ( 40 ).…”

Section: Discussionsupporting

confidence: 84%

Characterization of the early cellular immune response induced by HPV vaccines

et al. 2022

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IntroductionCurrent human papillomavirus (HPV) vaccines consist of virus-like particles (VLPs) which are based on the L1 protein, but they are produced by different expression systems and use different adjuvants. We performed in-depth immunophenotyping of multiple innate and adaptive immune cells after vaccination with bivalent versus nonavalent HPV vaccines.MethodTwenty pre-menopausal HPV-seronegative women were enrolled and randomized to receive three-doses of either the bivalent or the nonavalent HPV vaccine. Blood samples were collected at multiple time points from baseline up to 7 months after first vaccination. Four extensive EuroFlow flow cytometry antibody panels were used to monitor various immune cell subsets. Additionally, HPV-specific memory B- and T cells were determined by ELISPOT and HPV-specific antibody levels were measured by a VLP-based multiplex immunoassay.ResultsIn both cohorts, the numbers of plasma cells expanded in the first week after both primary and tertiary vaccination. HPV16 and HPV18-specific antibody levels and memory B and T-cell responses were higher in the bivalent than in the nonavalent vaccinees one month post third vaccination. For HPV31 and HPV45-specific antibody levels this pattern was reversed. Monocytes showed an expansion one day after vaccination in both cohorts but were significantly higher in the bivalent vaccine cohort. Large heterogeneity in responses of the other cell subsets was observed between donors.ConclusionThis pilot study showed a consistent response of monocytes and plasma cells after vaccination and a considerable variation in other circulating immune cells in both types of HPV vaccines between donors.

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Section: Discussionsupporting

confidence: 84%

Characterization of the early cellular immune response induced by HPV vaccines

et al. 2022

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“…Using this approach, not only the frequency of these cells can be established within peripheral blood mononuclear cells (PBMC) revealing the extent of their clonal sizes, but the assay is also suited to reveal the antibody classes and subclasses that these B cells produce, providing insights into the effector functions of B cell memory. While tetramers and other multimers can be used for the detection and study of rare antigen-specific T cells in PBMC [ 13 ], ELISPOT has been the primary approach for B cell immune monitoring and has been used to assess B cell memory in various antigenic and pathogenic systems [ 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody

Fecher

Caspell²,

Naeem³

et al. 2018

Cells

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In individuals who have once developed humoral immunity to an infectious/foreign antigen, the antibodies present in their body can mediate instant protection when the antigen re-enters. Such antigen-specific antibodies can be readily detected in the serum. Long term humoral immunity is, however, also critically dependent on the ability of memory B cells to engage in a secondary antibody response upon re-exposure to the antigen. Antibody molecules in the body are short lived, having a half-life of weeks, while memory B cells have a life span of decades. Therefore, the presence of serum antibodies is not always a reliable indicator of B cell memory and comprehensive monitoring of humoral immunity requires that both serum antibodies and memory B cells be assessed. The prevailing view is that resting memory B cells and B cell blasts in peripheral blood mononuclear cells (PBMC) cannot be cryopreserved without losing their antibody secreting function, and regulated high throughput immune monitoring of B cell immunity is therefore confined to—and largely limited by—the need to test freshly isolated PBMC. Using optimized protocols for freezing and thawing of PBMC, and four color ImmunoSpot® analysis for the simultaneous detection of all immunoglobulin classes/subclasses we show here that both resting memory B cells and B cell blasts retain their ability to secrete antibody after thawing, and thus demonstrate the feasibility of B cell immune monitoring using cryopreserved PBMC.

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“…In addition to antigen presentation by T cells, B cells play some roles in the humoral immune response after HB vaccination. The number of HBsAg‐specific B cells was reported to be significantly higher in the high responder group compared to that in the low responder group after HB booster vaccination, and Th2 cell activity was different between high and low responders with respect to IL‐13 production, indicating that HBsAg‐specific Th2 cell activity enhanced the differences in anti‐HBs levels and memory B‐cell responses . In an analysis of the B‐cell repertoire following naïve or booster HB vaccination, B cell‐driven immunity was confirmed by increases in vaccine‐specific plasma cells and memory cells .…”

Section: Discussionmentioning

confidence: 98%

GWAS identifying HLA‐DPB1 gene variants associated with responsiveness to hepatitis B virus vaccination in Koreans: Independent association of HLA‐DPB104:02* possessing rs1042169 G ‐ rs9277355 C ‐ rs9277356 A

Chung

Roh

Park

et al. 2019

Journal of Viral Hepatitis

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Recently, HLA class II loci, including HLA-DPB1, have been reported to be associated with interindividual variance in the hepatitis B (HB) vaccine response. In this study, we investigated significant single nucleotide polymorphisms (SNPs) for anti-HBs antibody levels in 6867 healthy Koreans using a genome-wide association study (GWAS).In GWAS, the top 20 SNPs that showed significant association with anti-HBs levels (P < 1.0 × 10 −29 ) all resided in HLA-DPB1. Utilizing PCR sequencing, we verified the relationship of the top 3 most significant SNPs (rs1042169, rs9277355 and rs9277356) from the GWAS and genotypes of HLA-DPB1 with the HB vaccine response in Korean infants who received a scheduled vaccination. The DPB1*04:02 allele has G, C and A nucleotides for the 3SNP sites, and was significantly more frequent in responders than in nonresponders (10.9% vs 1.0%, P c = 0.018). DPB1*05:01 was significantly more frequent in nonresponders than in responders (49.0% vs 31.1%, P c = 0.018).In multivariate logistic regression, DPB1*04:02 showed a significant association with both vaccine response (P = 0.037, OR = 8.465) and high-titre response (P = 0.027, OR = 9.860). The haplotypes rs1042169 G -rs9277355 C -rs9277356 A showed a significant association with a high-titre response only (P = 0.002, OR = 2.941). In conclusion, DPB1*04:02 possessing rs1042169 G -rs9277355 C -rs9277356 A is an independent predictor of the HB vaccine response in Koreans. K E Y W O R D Shepatitis B virus, HLA-DPB1, Korean, vaccine response

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Higher numbers of memory B-cells and Th2-cytokine skewing in high responders to hepatitis B vaccination

Cited by 14 publications

References 33 publications

Characterization of the early cellular immune response induced by HPV vaccines

Characterization of the early cellular immune response induced by HPV vaccines

B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody

GWAS identifying HLA‐DPB1 gene variants associated with responsiveness to hepatitis B virus vaccination in Koreans: Independent association of HLA‐DPB104:02* possessing rs1042169 G ‐ rs9277355 C ‐ rs9277356 A

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Higher numbers of memory B-cells and Th2-cytokine skewing in high responders to hepatitis B vaccination

Cited by 14 publications

References 33 publications

Characterization of the early cellular immune response induced by HPV vaccines

Characterization of the early cellular immune response induced by HPV vaccines

B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody

GWAS identifying HLA‐DPB1 gene variants associated with responsiveness to hepatitis B virus vaccination in Koreans: Independent association of HLA‐DPB1*04:02 possessing rs1042169 G ‐ rs9277355 C ‐ rs9277356 A

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GWAS identifying HLA‐DPB1 gene variants associated with responsiveness to hepatitis B virus vaccination in Koreans: Independent association of HLA‐DPB104:02* possessing rs1042169 G ‐ rs9277355 C ‐ rs9277356 A