Higher miR‐543 levels correlate with lower <i>STK31</i> expression and longer pancreatic cancer survival

Yuan, Weiping; Gao, Hao; Wang, Guangfu; Miao, Yi; Jiang, Kuirong; Zhang, Kai; Wu, Junli

doi:10.1002/cam4.3559

Cited by 12 publications

(10 citation statements)

References 37 publications

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“…To further explore the function of microRNA-543, we used published bioinformatics articles and online software prediction and found that microRNA-543 acts by targeting STK31 ( 22 ). In our study, we further verified the relationship between microRNA-543 and STK31.…”

Section: Resultsmentioning

confidence: 99%

“…Wang et al showed that LncRNA PVT1 controls TRPS1 transcription in breast cancer by absorbing microRNA-543 ( 27 ). Yuan et al found that microRNA-543 was associated with the occurrence and prognosis of PC by targeting STK31 through a comprehensive bioinformatics analysis ( 22 ). However, they did not perform a thorough study, while we performed a deep study of the mechanism of this result.…”

Section: Discussionmentioning

confidence: 99%

“…The StarBase 3.0 ( http://starbase.sysu.edu.cn ) database and Kaplan-Meier plotter ( http://kmplot.com/analysis/index.php?p=service ) also showed the same results. Furthermore, RNA22 ( https://cm.jefferson.edu/rna22/ ) and microRNA.org ( http://www.microrna.org/ ) predicted that microRNA-543 interacted with STK31, and previous research also reported that microRNA-543 plays a role in PC by targeting STK31 ( 22 ). Then, we selected STK31 as a target gene of microRNA-543 for further research.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis

Liu

Wei

Wang

et al. 2022

J Gastrointest Oncol

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Background: Pancreatic cancer (PC) is one of the most malignant cancers of the gastrointestinal tract.However, the study of targeted therapy research in PC is not very thorough. Therefore, targeted molecular markers are needed to aid in the diagnosis and treatment of PC. Methods: In our research, we investigated the biological functions and molecular mechanism of microRNA-543 in PC. Western blotting (WB) and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to analyze the transcription and protein expression of microRNA-543, Serine/threonine kinase 31 (STK31), and LINC00847 in BxPC-3 and PANC-1 cells. Subsequently, Cell Counting Kit-8 (CCK-8), Transwell, colony formation, and flow cytometry (FCM) assays were utilized to evaluate cell growth, migration, invasion, and apoptosis. WB and fluorescence in-situ hybridization (FISH) were used to evaluate the epithelialmesenchymal transition (EMT) process and subcellular localization. RNA immunoprecipitation (RIP), double luciferase reporter, and RNA-pull down assays were performed to determine the targeting relationship between microRNA-543 and STK31 or microRNA-543 and LINC00847.Results: While microRNA-543 expression was discovered to be low in PC, LINC00847 and STK31 were overexpressed at significant levels. MicroRNA-543 knockdown dramatically increased PC cell growth, invasion, metastasis, and EMT, as well as decreased apoptosis in functional studies. Furthermore, microRNA-543 and STK31 were found to be mutual targets. LINC00847 acted as a molecular sponge for microRNA-543 and a competitive endogenous RNA (ceRNA) for STK31, thereby increasing STK-31 transcription.Conclusions: Our results suggest that microRNA-543, through the LINC00847/microRNA-543/STK31 axis, plays a role in the development of PC as a tumor suppressor. As a result, microRNA-543 may prove to be an effective diagnostic and therapeutic target for PC.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis

Liu

Wei

Wang

et al. 2022

J Gastrointest Oncol

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show abstract

“…To further explore the function of microRNA-543, basing on the published bioinformatics article, we got that microRNA-543 worked by targeting STK31 [21]. In our study, we further veri ed the relationship between microRNA-543 and STK31.…”

Section: Microrna-543 Control Pc Development By Targeting Stk31mentioning

confidence: 64%

“…Further, previous research had reported that microRNA-543 plays a role in PC by targeting STK31 [21]. Then, we selected STK31 as a target gene of microRNA-543 for further research.…”

Section: Bioinformatics Analysismentioning

confidence: 98%

MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis

Liu

Wei

Wang

et al. 2022

Preprint

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Background Pancreatic cancer (PC) is one of the most malignant cancers of the gastro-intestinal tract. However, the study of targeted immunity in pancreatic cancer is not very thorough. Therefore, targeted molecular markers are needed to solve the diagnosis and treatment of pancreatic cancer. Methods In our research, we investigated the biological functions and molecular mechanism of microRNA-543 in PC. qRT-PCR and western blotting (WB) was utilized to test the transcription of microRNA-543, STK31, and LINC00847 in BxPC-3 as well as PANC-1cells. Then CCK-8, Trans-well, colony formation assay and flow cytometry assay were utilized to detect cell growth in cell number, migration, invasion and apoptosis, WB and FISH were utilized to evaluate the epithelial–mesenchymal transition (EMT) process and cellular subcellular localization. RIP, double luciferase report and RNA-pull down assay were utilized to determine targeting relationship between microRNA-543 and STK31 or microRNA-543-LINC00847. Results While microRNA-543 transcription was discovered to be low in PC, LINC00847 and STK31 were overexpressed at significant levels. MicroRNA-543 knockdown dramatically increased PC cell growth in cell number, invasion, metastasis, and EMT, as well as decreased apoptosis, in functional research. Furthermore, microRNA-543 and STK31 are mutually targeted. LINC00847 worked as a molecular absorbent for microRNA-543, and a competitive endogenous RNA (ceRNA) for STK31, increasing STK-31 transcription. Conclusions We believe that microRNA-543, through the LINC00847-microRNA-543-STK31 axis, plays a role in the development of PC and that it is a tumor suppressor as well. As a result, MicroRNA-543 may prove to be an impactive diagnostic and therapeutic target in this illness.

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Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer

Gao

Cai

et al. 2022

Cancer Medicine

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Backgroud Pancreatic cancer (PC) is a highly invasive malignancy with extremely poor prognosis. STK31 has been identified as a cancer‐testis (CT) gene, but its function in PC has not been elucidated well. Methods The effect of STK31 on cell proliferation, migration and invasion was investigated by in vitro and in vivo experiments and total RNA sequencing and targeted bisulfite sequencing was applied to explore the potential regulatory mechanisms of STK31 in PC. Results By analysis of tissue samples and the clinicopathologic features, we found that STK31 was reactivated in PC and associated with poor prognosis. In addition, the vitro and vivo studies indicated that STK31 could promote PC progression by facilitating cell proliferation, migration and invasion, and the indication. Targeted Bisulfite Sequencing showed that STK31 was regulated by methylation. Furthermore, the results of total RNA sequencing suggested that STK31 was closely related to signal transduction, metabolism, and the immune system. Conclusions This study demonstrates that STK31 , as a CT gene, can promote the development of PC and is regulated by methylation. STK31 could be considered as a potential therapeutic target for PC.

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Higher miR‐543 levels correlate with lower STK31 expression and longer pancreatic cancer survival

Cited by 12 publications

References 37 publications

MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis

MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis

MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis

Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer

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