Higher implantation and live birth rates with laser zona pellucida breaching than thinning in single frozen-thawed blastocyst transfer

Liu, Chengjun; Su, Ketong; Shang, Wei; Ji, Huili; Yuan, Chenfeng; Cao, Maosheng; Li, Chunjin; Zhou, Xu

doi:10.1007/s10103-019-02946-7

Cited by 11 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As discussed in a recent systematic review, even when only using the laser-mediated approach, it is unclear whether embryos benefit from ZP drilling or thinning [ 2 ]. Furthermore, some studies have attempted to compare ZP drilling and ZP thinning; nevertheless, discrepancies in study power and the variation in AH technique made it difficult to define a better method [ 10 , 44 – 47 ].…”

Section: Discussionmentioning

confidence: 99%

The Current Practice of Assisted Hatching for Embryos in Fertility Centres: a General Survey

2022

View full text Add to dashboard Cite

At present, there is no standardised protocol for assisted hatching (AH) and the field is beset with contradictory data. We hypothesised that such contradiction may be related to inconsistencies in clinical practice. This study aimed to investigate the application, preferences, and variations of AH in current clinical practice prior to embryo transfer (AHpET) and biopsy (AHpBP). An online voluntary survey, consisted of 25 questions regarding different aspects of AH, was circulated amongst different fertility centres via newsletters between October 2019 and March 2020. One-hundred twenty-nine different fertility centres participated in the survey. AHpBP was widely used (90.6% [48/53]) amongst these centres, especially for trophectoderm biopsy (92.2% [47/51]). In contrast, only 64.6% (73/113) of centres administrated AHpET; the application of AHpET was even lower in UK-based centres (36.6% [15/41]). Although laser pulses have become the predominant technique for AH, significant variation existed in the precise strategy. Zona pellucida (ZP) drilling was the main method for AHpBP, whilst both ZP drilling and ZP thinning were applied equally for AHpET. Furthermore, the ZP manipulation varied widely with regards to the size of the ZP opening and the extension of ZP thinning. This is the first representative survey relating to the current practice of AH. Laser-assisted AH is used extensively, especially for AHpBP. However, there is significant disparity in clinical practice across different centres. Future research should aim to create a standardised protocol for AH to help reduce the evident variation in clinical practice and investigate the true value of AH.

show abstract

Section: Discussionmentioning

confidence: 99%

The Current Practice of Assisted Hatching for Embryos in Fertility Centres: a General Survey

2022

View full text Add to dashboard Cite

show abstract

“…The follow‐up studies have been conducted to evaluate the effect of AH, including fresh cycles of cleavage‐stage embryos treated with acidic Tyrode's solution 27,28 and laser for ZP drilling, 29 and frozen cycles on cleavage‐stage embryos treated with laser ZP thinning 30,31 and on blastocyst stage treated with laser ZP thinning or bleaching 32 . AH would be considered a safe method of ART because it has no adverse effects on obstetrical and neonatal outcomes, including congenital malformation 30,32,33 . We evaluated neonatal outcome data of vitrified blastocysts with LAH from 93 babies and from 60 babies in the control group.…”

Section: Discussionmentioning

confidence: 99%

Laser‐assisted hatching on clinical and neonatal outcomes in patients undergoing single vitrified Blastocyst transfer: A propensity score–matched study

Endo

Mitsuhata

Hayashi

et al. 2021

Reprod Medicine & Biology

View full text Add to dashboard Cite

Purpose This study determined the effect of laser‐assisted hatching on the clinical and neonatal outcomes of single vitrified blastocyst transfer. Methods From June 2014 to March 2018, 289 matched pairs after propensity score matching were analyzed. During the blastocyst warming procedure, a small section of the zona pellucida area in the empty perivitelline space was sliced off using multiple laser beams. The clinical and neonatal outcomes of the laser‐treated group and non‐treatment control were analyzed. Results In the laser‐assisted hatching group, significantly higher rates of clinical pregnancy (40.8% vs 29.4%, P < .01) and live delivery (34.3% vs 22.5%, P < .01) were observed compared to the control group. Other variables such as the average gestational weeks, the sex of the baby, birthweight, or congenital malformations were found to have no significant differences in neonatal outcomes. Moreover, all babies were singleton live births. Conclusions Single vitrified blastocyst transfer treated with laser‐assisted hatching increases the live birth rate and has no adverse effects on neonatal outcomes.

show abstract

“…Despite the prevalence of both D-LAH and T-LAH in medical practice, determining the superior method remains contentious [ 22 ]. Existing studies present conflicting findings: some assert D-LAH's superiority, some favor T-LAH, while others report no substantial disparity [ 12 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…After applying exclusion criteria using EndNote, 209 studies met the requirements for quantitative analysis. These studies were selected based on the reviewers' evaluation of the titles, abstracts, and full texts of the remaining 205 studies [ 12 , 16 , 23 , 24 , 26 – 30 ]. The literature search specifically focused on English papers, as depicted in Fig.…”

Section: Introductionmentioning

confidence: 99%

Two laser-assisted hatching methods of embryos in ART: a systematic review and meta-analysis

Chen,

Gao,

et al. 2024

BMC Pregnancy Childbirth

View full text Add to dashboard Cite

Background Laser-assisted hatching (LAH) stands as the predominant technique for removing the zona pellucida (ZP) in embryos, primarily consisting of two methods: drilling laser-assisted hatching (D-LAH) and thinning laser-assisted hatching (T-LAH). Presently, both methods have limitations, and their comparative efficacy for embryo implantation and clinical pregnancy remains uncertain. Aim Evaluate the impact of D-LAH and T-LAH on clinical pregnancy rates within assisted reproductive technology (ART). Methods We systematically searched electronic databases including PubMed, Web of Science, and Cochrane Library until July 20, 2022. This study encompassed observational studies and randomized controlled trials (RCTs). A 95% confidence interval (CI) was utilized for assessing the risk ratio (RR) of pregnancy outcomes. The level of heterogeneity was measured using I2 statistics, considering a value exceeding 50% as indicative of substantial heterogeneity. Results The meta-analysis scrutinized 9 studies involving 2405 clinical pregnancies from D-LAH and 2239 from T-LAH. Findings suggested no considerable variation in the clinical pregnancy rates between the two techniques (RR = 0.93, 95% CI: 0.79–1.10, I2 = 71%, P = 0.41). Subgroup analyses also revealed no substantial differences. However, D-LAH exhibited a notably higher occurrence of singleton pregnancies compared to T-LAH (RR = 2.28, 95% CI: 1.08–4.82, I2 = 89%, P = 0.03). There were no noteworthy distinctions observed in other secondary outcomes encompassing implantation rate, multiple pregnancies, ongoing pregnancy, miscarriage, premature birth, and live birth. Conclusion Both the primary findings and subgroup analyses showed no marked variance in clinical pregnancy rates between D-LAH and T-LAH. Therefore, patients with varying conditions should select their preferred LAH technique after assessing their individual situation. However, due to the restricted number of studies involved, accurately gauging the influence of these laser techniques on clinical outcomes is challenging, necessitating further RCTs and high-quality studies to enhance the success rate of ART. Trial registration PROSPERO: CRD42022347066.

show abstract

Higher implantation and live birth rates with laser zona pellucida breaching than thinning in single frozen-thawed blastocyst transfer

Cited by 11 publications

References 24 publications

The Current Practice of Assisted Hatching for Embryos in Fertility Centres: a General Survey

The Current Practice of Assisted Hatching for Embryos in Fertility Centres: a General Survey

Laser‐assisted hatching on clinical and neonatal outcomes in patients undergoing single vitrified Blastocyst transfer: A propensity score–matched study

Two laser-assisted hatching methods of embryos in ART: a systematic review and meta-analysis

Contact Info

Product

Resources

About