Higher efficacy of dietary DHA provided as a phospholipid than as a triglyceride for brain DHA accretion in neonatal piglets

Liu, Lei; Bartke, Nana; Daele, Hans Van; Lawrence, Peter; Qin, Xia; Park, Hui Gyu; Kothapalli, Kumar S.D.; Windust, Anthony; Bindels, Jacques; Wang, Zhe; Brenna, J. Thomas

doi:10.1194/jlr.m045930

Cited by 90 publications

(60 citation statements)

References 59 publications

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“…In this study, we reinforced this conclusion. Taken together, DHA‐PC had a better effect on DHA accretion in erythrocytes than DHA‐TG, consistent with previous studies (Valenzuela and others ; Liu and others ). These results may be related to the DHA processing and repackaging in the liver, and dietary DHA‐PC could increase the LysoPC‐DHA level in albumin (Lagarde and others ), which would exchange the lipids with erythrocytes phospholipids in the circulation, potentially contributing to a higher DHA level in erythrocytes, also indicating a increase of DHA level in the brain.…”

Section: Resultssupporting

confidence: 92%

Replenishment of Docosahexaenoic Acid (DHA) in Dietary n‐3‐Deficient Mice Fed DHA in Triglycerides or Phosphatidylcholines After Weaning

Wang

Wen

et al. 2018

Journal of Food Science

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Dietary DHA is essential for neurodevelopment which is usually accompanied by large amounts of DHA accretion in the brain. Our present study showed that DHA-PC had a better efficiency for DHA accretion in the brain and other tissues compared with DHA-TG. The findings are supposed to pave the way for the DHA in phospholipids as a novel nutrient added into the infant formula and assisted food for neurodevelopment.

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Section: Resultssupporting

confidence: 92%

Replenishment of Docosahexaenoic Acid (DHA) in Dietary n‐3‐Deficient Mice Fed DHA in Triglycerides or Phosphatidylcholines After Weaning

Wang

Wen

et al. 2018

Journal of Food Science

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“…Visualized bands were carefully collected with known amount of heneicosanoic acid (21:0) as internal standard, then saponified and methyl-ester prepared with 14% BF 3 in methanol (Sigma-Aldrich, St. Louis, MO). Fatty acids methyl esters (FAME) were analyzed by GC-FID and identified by GC-MS/MS as described elsewhere [13]. Response factors using an external standard mixture was used applied to the raw data to correct for differential instrument response.…”

Section: Methodsmentioning

confidence: 99%

Human fetal intestinal epithelial cells metabolize and incorporate branched chain fatty acids in a structure specific manner

Liu

Wang

Park

et al. 2017

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Background Branched chain fatty acids (BCFA) are constituents of gastrointestinal (GI) tract in healthy newborn human infants, reduce the incidence of necrotizing enterocolitis (NEC) in a neonatal rat model, and are incorporated into small intestine cellular lipids in vivo. We hypothesize that BCFA are taken up, metabolized and incorporated into human fetal cells in vitro. Methods Human H4 cells, a fetal non-transformed primary small intestine cell line, were incubated with albumin-bound non-esterified anteiso-17:0, iso-16:0, iso-18:0 and/or iso-20:0, and FA profiles in lipid fractions were analyzed. Results All BCFA were readily incorporated as major constituents of cellular lipids. Anteiso-17:0 was preferentially taken up, and was most effective among BCFA tested in displacing normal (n-) FA. The iso BCFA were preferred in reverse order of chain length, with iso-20:0 appearing at lowest level. BCFA incorporation in phospholipids (PL) followed the same order of preference, accumulating 42% of FA as BCFA with no overt morphological signs of cell death. Though cholesterol esters (CE) are at low cellular concentration among lipid classes examined, CE had the greatest affinity for BCFA, accumulating 65% of FA as BCFA. BCFA most effectively displaced lower saturated FA. Iso-16:0, iso-18:0 and anteiso-17:0 were both elongated and chain shortened by ± C2. Iso-20:0 was chain shortened to iso-18:0 and iso-16:0 but not elongated. Conclusions Nontransformed human fetal intestinal epithelial cells incorporate high levels of BCFA when they are available and metabolize them in a structure specific manner. These findings imply that specific pathways for handling BCFA are present in the lumen-facing cells of the human fetal GI tract that is exposed to vernix-derived BCFA in late gestation.

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“…While fish oil mainly contains triglyceride‐ bound n‐3 PUFAs, in krill oil the main part of these fatty acids is bound to phospholipids, with phosphatidylcholine being the most abundant form . This feature could be biologically relevant, based on the increasing evidence that phospholipids might be a more effective delivery form of n‐3 PUFAs to body tissues than triglycerides . The health benefits of krill oil have been the object of many animal studies, however a possible effect on atherosclerosis has not been investigated yet.…”

Section: Introductionmentioning

confidence: 99%

Effect of Dietary Components from Antarctic Krill on Atherosclerosis in apoE‐Deficient Mice

Parolini

Bjørndal

Busnelli

et al. 2017

Molecular Nutrition Food Res

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Higher efficacy of dietary DHA provided as a phospholipid than as a triglyceride for brain DHA accretion in neonatal piglets

Cited by 90 publications

References 59 publications

Replenishment of Docosahexaenoic Acid (DHA) in Dietary n‐3‐Deficient Mice Fed DHA in Triglycerides or Phosphatidylcholines After Weaning

Replenishment of Docosahexaenoic Acid (DHA) in Dietary n‐3‐Deficient Mice Fed DHA in Triglycerides or Phosphatidylcholines After Weaning

Human fetal intestinal epithelial cells metabolize and incorporate branched chain fatty acids in a structure specific manner

Effect of Dietary Components from Antarctic Krill on Atherosclerosis in apoE‐Deficient Mice

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