Higher biomolecules yield in phytoplankton under copper exposure

Silva, Jaqueline Carmo; Echeveste, Pedro; Lombardi, Ana Teresa

doi:10.1016/j.ecoenv.2018.05.059

Cited by 39 publications

(25 citation statements)

References 65 publications

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“…Variation in the concentration of molecules related to the energy metabolism in organisms at the base of food webs like microalgae is of great environmental significance, because energy imbalance in aquatic environments may be a consequence. In contrast to our study, Barreto and Lombardi () showed no variation in carbohydrates in Scenedesmus bijugus exposed to TiO 2 ‐NPs, but Silva et al () showed carbohydrate increase in Cu ion–contaminated Scenedesmus quadricauda . So, in addition to Cu ion toxicity from the dissolution of the Cu‐NPs that occured in the media, another effect may have originated from the NPs specifically.…”

Section: Discussioncontrasting

confidence: 99%

“…The literature shows a tendency toward lipid increase after metal‐induced stress in microalgae (Chia et al , ; Rocha et al ); however, the concentrations that induced lipid accumulation in those studies were always higher and the exposure times usually longer than those adopted in the present study. Silva et al () showed that at up to 10 −6 mol L −1 Cu, total lipid content in S. quadricauda remained similar to the control, but increased at 2.5 × 10 −6 mol L −1 and higher, whereas half that metal amount was necessary to affect carbohydrates and proteins. For comparison, our highest free Cu concentration in the medium that originated from the dissolution of the Cu‐NPs was 2.6 × 10 −8 mol L −1 , approximately 100 times lower.…”

Section: Discussionmentioning

confidence: 95%

“…In addition, it assists in the signaling/transcription of proteins, and in their transport, mobilization, and oxidative phosphorylation (Robinson and Winge ). Previous studies have reported on the effects of Cu in microalgae and have shown that in addition to growth (Lombardi and Maldonado, ), the accumulation (Echeveste et al ; Rocha et al ) and productivity (Silva et al ) of biomolecules such as carbohydrates and proteins are affected. Although the toxicity of Cu has been shown to vary with microalgae species and exposure time (Franklin et al ; Moreno‐Garrido et al ), its effect on photosynthesis has been widely demonstrated (Araujo et al ).…”

Section: Introductionmentioning

confidence: 99%

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Physiological responses of Chlorella sorokiniana to copper nanoparticles

Barreto

Tonietto

Amaral

et al. 2019

Enviro Toxic and Chemistry

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Copper (Cu) nanomaterials have been increasingly researched and produced for many different consumer products. They have high reactivity and bactericidal properties, making them important in antifouling paints, which are thus directly introduced into aquatic ecosystems. However, studies are scarce on the behavior of Cu nanoparticles (Cu-NPs) in natural aquatic systems and their interactions with primary producers such as microalgae. We investigated the effects of NPs on some physiological responses of the freshwater phytoplankton Chlorella sorokiniana. The cells were exposed to nominal concentrations ranging from 2.50 to 635.00 mg L À1 Cu-NPs for 96 h under laboratory-controlled conditions. The cultures were monitored daily for population growth and maximum photosynthetic quantum yield. Total lipids, proteins, and carbohydrates were quantified at 72 h of Cu-NP exposure. The results showed a positive correlation between nominal Cu-NPs and Cu in the biomass (0.97 correlation coefficient) and that this was inversely proportional to total carbohydrates, with a -0.64 correlation coefficient. At the higher end of the Cu-NP concentrations tested, higher total proteins and reduced growth rates were obtained in comparison with controls; we suggest that metal-binding proteins/antioxidants and nonstructural proteins were preferentially produced under these conditions. Our results contribute to an understanding of the interaction between Cu-NPs and a cosmopolitan phytoplankton, C. sorokiniana, and we emphasize that the disposal and use of Cu-NPs requires monitoring because even at environmentally relevant concentrations, the composition of the algae was affected. Environ Toxicol Chem 2019;38:387-395. C 2018 SETAC

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Physiological responses of Chlorella sorokiniana to copper nanoparticles

Barreto

Tonietto

Amaral

et al. 2019

Enviro Toxic and Chemistry

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“…Silva et al. () showed increased total carbohydrates and proteins in Scenedesmus quadricauda exposed to copper. Industries whose aim is continuous harvesting of biomass are interested in biomolecule accumulation, without reducing growth rate, a situation that will result in higher daily biomolecule yield.…”

mentioning

confidence: 98%

“…, Silva et al. ). The influence of Cd on the aminoacid profile of Chlorella vulgaris was described by Chia et al.…”

mentioning

confidence: 99%

The effects of copper on photosynthesis and biomolecules yield in Chlorolobion braunii

Baracho

Silva

Lombardi

2019

Journal of Phycology

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Our knowledge of the effects of copper on microalgal physiology is largely based on studies conducted with high copper concentrations; much less is known when environmentally relevant copper levels come into question. Here, we evaluated the physiology of Chlorolobion braunii exposed to free copper ion concentrations between 5.7 × 10−9 and 5.0 × 10−6 mol · L−1, thus including environmentally relevant values. Population growth and maximum photosynthetic quantum yield of PSII were determined daily during the 96 h laboratory controlled experiment. Exponentially‐growing cells (48 h) were analyzed for effective quantum yield and rapid light curves (RLC), and total lipids, proteins, carbohydrates, chlorophyll a and carotenoids were determined. The results showed that growth rates and population density decreased gradually as copper increased in experiment, but the photosynthetic parameters (maximum and effective quantum yields) and photochemical quenching (qP) decreased only at the highest free copper concentration tested (5.0 × 10−6 mol · L−1); nonphotochemical quenching (NPQ) increased gradually with copper increase. The RLC parameters Ek and rETRmax were inversely proportional to copper concentration, while α and Im decreased only at 5.0 × 10−6 mol · L−1. The effects of copper in biomolecules yield (mg · L−1) varied depending on the biomolecule. Lipid yield increased at free copper concentration as low as 2.5 × 10−8 mol · L−1, but proteins and carbohydrates were constant throughout.

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Individual and Combined Effects of Manganese and Chromium on a Freshwater Chlorophyceae

Alho

Gebara

Mansano

et al. 2022

Enviro Toxic and Chemistry

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Manganese (Mn), an essential metal in trace amounts, and chromium (Cr), a nonessential metal to algae, are often found in effluent discharges and may co‐occur in contaminated aquatic environments. Therefore, we investigated the effects of Mn and Cr, and their mixtures, on a freshwater Chlorophyceae, Raphidocelis subcapitata, using a multiple endpoint approach. Regarding the single exposure of metals, Mn was 4 times more toxic (median inhibitory concentration at 72 h [IC5072 h] = 4.02 ± 0.45 µM Mn) than Cr (IC5072 h = 16.42 ± 4.94 µM Cr) for microalgae, considering the effects on the relative growth rate. Moreover, this species was the most sensitive to Mn, according to the species sensitivity distribution curve. Overall, the tested metals did not lead to significant changes in reactive oxygen species production, cellular complexity, and cell relative size but significantly decreased the algal growth and the mean cell chlorophyll a (Chl a) fluorescence at the highest concentrations (3.64–14.56 µM of Mn and 15.36–19.2 µM of Cr). The decreased mean cell Chl a fluorescence indicates an impact on pigment synthesis, which may be related to the observed growth inhibition. In the mixture tests, the reference models concentration addition and independent action were used to analyze the data, and the independent action model was the best fit to describe our results. Therefore, the Mn and Cr mixture was noninteractive, showing additive effects. This is the first study to address the combined toxicity of Mn and Cr regarding freshwater Chlorophyceae. Environ Toxicol Chem 2022;41:1004–1015. © 2022 SETAC

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Higher biomolecules yield in phytoplankton under copper exposure

Cited by 39 publications

References 65 publications

Physiological responses of Chlorella sorokiniana to copper nanoparticles

Physiological responses of Chlorella sorokiniana to copper nanoparticles

The effects of copper on photosynthesis and biomolecules yield in Chlorolobion braunii

Individual and Combined Effects of Manganese and Chromium on a Freshwater Chlorophyceae

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