Higher biofilm formation in multidrug-resistant clinical isolates of Staphylococcus aureus

Kwon, An Sung; Park, Gwang Chul; Ryu, So Yeon; Lim, Dong Hoon; Lim, Dong Yoon; Choi, Chul Hee; Park, Yoonkyung; Lim, Yong

doi:10.1016/j.ijantimicag.2008.02.009

Cited by 92 publications

(74 citation statements)

References 15 publications

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“…SCCmec IV isolates were capable of higher biofilm formation (Kwon et al, 2008). In contrast to the findings by Lim et al where SCCmec III was found to be a genetic risk factor for strong biofilm formation (Lim et al, 2013), we found SCCmec IV isolates produced the strongest biofilms (only three isolates tested).…”

Section: Discussioncontrasting

confidence: 99%

“…A larger collection of MRSA isolates should be tested to make any meaningful conclusions. (Kwon et al, 2008). Other studies showed no difference in biofilm formation between MRSA and MSSA isolates (Smith et al, 2008;Indrawattana et al, 2013).…”

Section: Discussionmentioning

confidence: 94%

“…Isolates from blood cultures showed a higher frequency of biofilm formation compared to isolates from other sites (Kwon et al, 2008). In the study by Smith et al, the isolates derived from skin had a greater ability to form fully established biofilms (Smith et al, 2008).…”

Section: Discussionmentioning

confidence: 94%

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Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Naicker

Karayem

Hoek

et al. 2016

Microbial Pathogenesis

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2 Declaration I, Preneshni Rochelle Naicker, hereby declare that the work contained in this assignment is my original work and that I have not previously submitted it, in its entirety or in part, at any university for a degree. The ability to form biofilms contributes significantly to the virulence of Staphylococcus aureus. Virulence factors may be associated with certain S. aureus lineages. However, the contribution of the genetic background of S. aureus to biofilm formation is poorly understood. This study investigated the association between the genetic background and the biofilm forming ability of clinical invasive S. aureus isolates. Secondary objectives included investigating any correlation with biofilm formation and methicillin resistance or the source of bacteraemia. Methods:The study was conducted at a 1300-bed tertiary hospital in Cape Town, South Africa. S. aureus isolates obtained from blood cultures between January 2010 and January 2012 were included. Genotypic characterization was performed by PFGE, spa typing, SCCmec typing and MLST. Thirty genotypically unique strains were assessed for phenotypic biofilm formation with the microtitre plate assay. All isolates were tested in triplicate and an average optical density, measured at a wavelength of 490nm, was determined. The biofilm forming ability of isolates with A 490 > 0.17 was considered 'weak positive' and A 490 > 0.34 'strong positive'. Isolates with A 490 ≤ 0.17 were considered non-adherent. ANOVA with Bonferroni-adjusted post-hoc tests and t-tests were used for statistical analysis of the association between biofilm forming ability and strain characteristics.Results: Fifty seven percent of isolates were capable of forming biofilms. Weak biofilm formation occurred in 40% (n=12) and strong biofilm formation in 17% (n=5) of isolates. Thirteen (43%) of the isolates were classified as non-adherent. All 5 isolates capable of strong biofilm formation belong to one spa clonal complex (spa-CC 064). Strains from spa-CC 064 were capable of higher biofilm formation than other spa clonal complexes (p=0.00002). These 5 strains belonged to MLST CC5 and CC8. Biofilm formation did not correlate with methicillin resistance and was not related to the source of bacteraemia. Conclusion:Biofilm formation correlates with the spa clonal lineage in our population of invasive S. aureus strains. High biofilm formation was associated with spa-CC 064. MLST CC5 and CC8 strains are capable of strong biofilm formation.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 94%

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Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Naicker

Karayem

Hoek

et al. 2016

Microbial Pathogenesis

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“…There was insignificant association between biofilm formation and methicillin resistant in Staphylococcus aureus. Similar study conducted by Kwon et al [12] showed the relationship between methicillin resistance and biofilm formation, they found that the rate of biofilm positivity was 37.9% for methicillin-resistant strains and 14.3% for methicillinsusceptible strains (P<0.05)…”

Section: Discussionsupporting

confidence: 76%

Effect of Chlorhexidine and Sodium Hypochlorite on Staphylococcus aureus Biofilm

Abdallah¹,

Abakar²

2017

J Prev Infect Cntrol

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Biofilm is a matrix in which a microorganism encases in it and survives environmental stresses. It help the organisms to resist the antibiotics and disinfectant, chronic biofilm associated infection lead to significant increase in morbidity and mortality especially patient with indwelling medical devices. The objective of this research was to analyze the effectiveness of chlorhexidine, sodium hypochlorite and antimicrobial activity of methicillin and vancomycin against biofilm of isolated strains of Staphylococcus aureus isolated from different clinical samples. The results revealed that most biofilm strains were sensitive to vancomycin, some strains were sensitive some were moderate resist and some were resist to methicillin. In comparing different concentrations (0.3%, 0.2%, 0.15% and 0.075%) of chlorhexidine among time interval (1 min, 3 min and 5 min) concentrations showed significant decrease in biofilm formation in association with time; P value (0.001, 0.001, 0.000 and 0.000, respectively). Different concentrations (5%, 4%, 2.5% and 1.25%) of sodium hypochlorite also tested through the same time intervals; concentrations showed significant decrease in biofilm in association with time; P value (0.000, 0.000, 0.000 and 0.000, respectively).

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“…Biofilms are closely involved in higher antibiotic resistance because of several conditions including lower penetration of antibiotics, lower growth rate of bacteria in biofilms and altered metabolic requirements. Eventually, the surviving isolates in the inner portion of biofilms are likely to possess a higher probability of acquiring the ability to develop biofilms as well as multidrug resistance in clinical settings (9). Biofilm forming strains are more frequently isolated from non-fluid tissues, in particular bone and soft tissues, and also MDR pathogens are more often biofilm formers.…”

Section: Introductionmentioning

confidence: 99%

Several Virulence Factors of Multidrug-Resistant Staphylococcus aureus Isolates From Hospitalized Patients in Tehran

Ghasemian¹,

Peerayeh²,

Bakhshi³

et al. 2015

Int J Enteric Pathog

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Background: Biofilm formation plays an important role in resistance of Staphylococcus aureus isolates; especially multidrug-resistant isolates are a threat to healthcare settings. Objectives: The aims of this study were to detect biofilm formation and presence of several related genes among multidrug-resistant (MDR) isolates of Staphylococcus aureus. Patients and Methods: A total Of 209 S. aureus strains were isolated from patients and identified by conventional diagnostic tests. The multidrug-resistant MRSA isolates were detected by antibiotic susceptibility test. The phenotypic biofilm formation was detected by microtitre tissue plate assay. The polymerase chain reaction (PCR) was performed to detect the mecA, Staphylococcal Cassette Chromosome mec (SCCmec) types, accessory gene regulatory (agr) genes, the icaADBC and several genes encoding staphylococcal surface proteins including clfAB, fnbAB, fib, eno, can, ebps and bbp genes with specific primers. Results: Sixty-four (30.6%) isolates were methicillin-resistant, among which thirty-six (56.2%) were MDR. These isolates were resistant to amoxicillin, tetracycline, ciprofloxacin, gentamicin, erythromycin and trimethoprim-sulfamethoxazole (except to 6 isolates). All the isolates were susceptible to vancomycin and linezolid. All the MDR-MRSA harbored SCCmec type III. All the MDR-MRSA isolates were strong biofilm producers in the phenotypic test. The majority of MDR-MRSA was belonged to agrI (67%, n = 24), followed by agr II (17%, n = 6), agrIV (11%, n = 4) and agrIII (5.5%, n = 2). The frequency of icaADBC genes were 75% (n = 27), 61% (n = 22), 72% (n = 26) and 72% (n = 26), respectively. Furthermore, the prevalence of clfA, clfB, fnbA, fnbB, fib, can, eno, ebps and bbp genes was 100%, 100%, 67%, 56%, 80%, 63%, 78%, 7% and 0%, respectively. Furthermore, approximately all the MRSA was strong biofilm producers. Conclusions: Multidrug-resistant isolates produced biofilm strongly and the majority harbored most of biofilm related genes, suggesting that biofilm formation is associated to the presence of these genes, and also biofilm production can increase the antibiotic resistance as have demonstrated in MDR-MRSA isolates.

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Higher biofilm formation in multidrug-resistant clinical isolates of Staphylococcus aureus

Cited by 92 publications

References 15 publications

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Effect of Chlorhexidine and Sodium Hypochlorite on Staphylococcus aureus Biofilm

Several Virulence Factors of Multidrug-Resistant Staphylococcus aureus Isolates From Hospitalized Patients in Tehran

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