High-yields heterologous production of the novel Aspergillus fumigatus elastase inhibitor AFUEI in Aspergillus oryzae

Yamashita, Nobuo; Komori, Yumiko; Okumura, Yoshiyuki; Uchiya, Kei-ichi; Matsui, Toshimitsu; Nishimura, Akira; Ogawa, Kenji; Nikai, Toshiaki

doi:10.1016/j.jbiosc.2011.03.020

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…Purification and Crystallization of AFUEI-Details of the expression and purification of AFUEI were previously described (16). The protein solution for crystallization was prepared by dissolving vacuum-dried AFUEI in distilled water (19 mg/ml).…”

Section: Methodsmentioning

confidence: 99%

X-ray Structure Analysis and Characterization of AFUEI, an Elastase Inhibitor from Aspergillus fumigatus

Sakuma

Imada

Okumura

et al. 2013

Journal of Biological Chemistry

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Background:Elastase is an important factor in aspergillosis, and AFUEI is an elastase inhibitor derived from Aspergillus fumigatus. Results: The structure of AFUEI, the first structure of the I78 inhibitor family, was determined. Conclusion:The structure of AFUEI is extremely similar to serine protease inhibitors of the potato inhibitor I family. Significance: Our findings provide a basic contribution to both the prevention and treatment for aspergillosis.

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Section: Methodsmentioning

confidence: 99%

X-ray Structure Analysis and Characterization of AFUEI, an Elastase Inhibitor from Aspergillus fumigatus

Sakuma

Imada

Okumura

et al. 2013

Journal of Biological Chemistry

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“…To improve the production of heterologous proteins, in addition to reducing the activity of proteases, a series of strong promoters were developed to induce the expression of heterologous genes (Table 2). In A. oryzae, the starch-inducible promoter of the alpha-amylase gene (PamyB) is one of the most commonly used promoters for the production of foreign proteins (Tsuchiya et al, 1992;Ward et al, 1992;Rey et al, 2003;Hoshida et al, 2005;Yamashita et al, 2011;Yin et al, 2015). The glucoamylase gene promoter (PglaA) and its improved promoter (PglaA142) were often used for the production and function analysis of recombinant proteins, such as Candida antarctica lipase B and A. oryzae recombinant tannase (Tamalampudi et al, 2007;Tokuoka et al, 2008;Ichikawa et al, 2020).…”

Section: Promoters and Endogenous Secretion Carrier Proteins For Hetementioning

confidence: 99%

Advances in Genetic Engineering Technology and Its Application in the Industrial Fungus Aspergillus oryzae

Jin

Wang

et al. 2021

Front. Microbiol.

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The filamentous fungus Aspergillus oryzae is an important strain in the traditional fermentation and food processing industries and is often used in the production of soy sauce, soybean paste, and liquor-making. In addition, A. oryzae has a strong capacity to secrete large amounts of hydrolytic enzymes; therefore, it has also been used in the enzyme industry as a cell factory for the production of numerous native and heterologous enzymes. However, the production and secretion of foreign proteins by A. oryzae are often limited by numerous bottlenecks that occur during transcription, translation, protein folding, translocation, degradation, transport, secretion, etc. The existence of these problems makes it difficult to achieve the desired target in the production of foreign proteins by A. oryzae. In recent years, with the decipherment of the whole genome sequence, basic research and genetic engineering technologies related to the production and utilization of A. oryzae have been well developed, such as the improvement of homologous recombination efficiency, application of selectable marker genes, development of large chromosome deletion technology, utilization of hyphal fusion techniques, and application of CRISPR/Cas9 genome editing systems. The development and establishment of these genetic engineering technologies provided a great deal of technical support for the industrial production and application of A. oryzae. This paper reviews the advances in basic research and genetic engineering technologies of the fermentation strain A. oryzae mentioned above to open up more effective ways and research space for the breeding of A. oryzae production strains in the future.

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“…Additionally, the successful heterologous expressions of important structural natural products documented in the literature have been achieved through A. oryzae (Table 2). Furthermore, the heterologous expressions of these compounds have resulted in higher yields than those produced using their original strains [15]. For example, the heterogeneous expression of the trili biosynthetic gene clusters of Trichoderma reesei in A. oryzae produced new compounds (two acyl tetramic acids), indicating the fungal host diversity in catalytic reactions [16].…”

Section: Introductionmentioning

confidence: 99%

Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in Aspergillus oryzae

Jia,

Song,

et al. 2024

JoF

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The filamentous fungus Aspergillus oryzae (A. oryzae) has been extensively used for the biosynthesis of numerous secondary metabolites with significant applications in agriculture and food and medical industries, among others. However, the identification and functional prediction of metabolites through genome mining in A. oryzae are hindered by the complex regulatory mechanisms of secondary metabolite biosynthesis and the inactivity of most of the biosynthetic gene clusters involved. The global regulatory factors, pathway-specific regulatory factors, epigenetics, and environmental signals significantly impact the production of secondary metabolites, indicating that appropriate gene-level modulations are expected to promote the biosynthesis of secondary metabolites in A. oryzae. This review mainly focuses on illuminating the molecular regulatory mechanisms for the activation of potentially unexpressed pathways, possibly revealing the effects of transcriptional, epigenetic, and environmental signal regulation. By gaining a comprehensive understanding of the regulatory mechanisms of secondary metabolite biosynthesis, strategies can be developed to enhance the production and utilization of these metabolites, and potential functions can be fully exploited.

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High-yields heterologous production of the novel Aspergillus fumigatus elastase inhibitor AFUEI in Aspergillus oryzae

Cited by 5 publications

References 11 publications

X-ray Structure Analysis and Characterization of AFUEI, an Elastase Inhibitor from Aspergillus fumigatus

X-ray Structure Analysis and Characterization of AFUEI, an Elastase Inhibitor from Aspergillus fumigatus

Advances in Genetic Engineering Technology and Its Application in the Industrial Fungus Aspergillus oryzae

Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation in Aspergillus oryzae

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