High-yielding, one-pot, and green production of biodiesel from waste grease using wet cells of a recombinant Escherichia coli strain as catalyst

Tian, Kaiyuan; Li, Zhi

doi:10.1016/j.bej.2016.08.002

Cited by 10 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among them, high 2-PE conversion was observed with ethyl oleate and biodiesel producing 66.8 mM (95.2% conversion) and 65.4 mM (93.2% conversion) 2-PE, respectively. Though first generation biodiesel obtained from edible sources such as palm oil are not sustainable due to its competition for food and fertile land, researchers have developed second generation biodiesel from sustainable, nonedible sources such as waste cooking oil, waste grease etc. − …”

Section: Resultsmentioning

confidence: 99%

“…In the meantime, E. coli T7-Sty was grown at 22 °C to produce cells containing the L-Phe to 2-PE enzyme cascades. According to our previous study, 39 15 g cdw/L of cells is optimal for the conversion of 50−80 mM L-Phe to 2-PE. Therefore, 15 g cdw/ L of active E. coli T7-Sty cells was obtained by centrifugation and added to the 10 mL of fermentation culture containing 70.2 mM L-Phe.…”

Section: Acs Sustainable Chemistry and Engineeringmentioning

confidence: 95%

“…An alternative way of producing natural 2-PE is the microbial synthesis from glucose or glycerol which are green and sustainable sources. Glucose can be obtained from nonedible lignocellulosic biomass such as sugarcane bagasse. , Glycerol can be also considered as a sustainable source when obtained as a byproduct from second generation biodiesel synthesis using nonedible sources such as waste cooking oil , and waste grease. , In addition, biocatalysts are environmental-friendly, nontoxic and biodegradable. Several microorganisms including yeast were reported for the fermentative production of 2-PE from glucose, , but at very low concentration.…”

Section: Introductionmentioning

confidence: 99%

“…10,11 Glycerol can be also considered as a sustainable source when obtained as a byproduct from second generation biodiesel synthesis using nonedible sources such as waste cooking oil 12,13 and waste grease. 14,15 In addition, biocatalysts are environmental-friendly, nontoxic and biodegradable. Several microorganisms including yeast were reported for the fermentative production of 2-PE from glucose, 5,16 but at very low concentration.…”

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Production of Natural 2-Phenylethanol from Glucose or Glycerol with CoupledEscherichia coliStrains Expressingl-Phenylalanine Biosynthesis Pathway and Artificial Biocascades

Sekar

Lukito

2019

ACS Sustainable Chem. Eng.

Self Cite

View full text Add to dashboard Cite

2-Phenylethanol (2-PE) is a fragrance widely used in food and cosmetics. Natural 2-PE is preferred in these applications but with high price and very limited availability. Microbial synthesis from glucose or glycerol is an attractive way to produce natural 2-PE, but suffers from low product titer. Escherichia coli NST74-Phe-Sty was engineered to coexpress the L-phenylalanine (L-Phe) biosynthesis pathway and enzyme cascade of L-Phe to 2-PE, producing only 0−0.17 g/L 2-PE from glucose at 22−37 °C due to the incompatibility of the temperature for enzyme expression and activity. Enhanced production of 2-PE (8.4−9.1 g/L) from glucose or glycerol was achieved by coupling of E. coli NST74-Phe expressing the L-Phe biosynthesis pathway for L-Phe production at the optimal temperature of 37 °C with E. coli T7-Sty expressing enzyme cascades of L-Phe to 2-PE at the optimal expression temperature of 22 °C and the optimal biotransformation temperature of 30 °C. The 2-PE titer is 4.7-fold higher than the best reported 2-PE concentration produced from glucose. The coupled strains approach could be generally applicable for enhancing microbial production of useful chemicals from sugars or glycerol.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Acs Sustainable Chemistry and Engineeringmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production of Natural 2-Phenylethanol from Glucose or Glycerol with CoupledEscherichia coliStrains Expressingl-Phenylalanine Biosynthesis Pathway and Artificial Biocascades

Sekar

Lukito

2019

ACS Sustainable Chem. Eng.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Normally, the FFA content of wastewater feedstock ranges from 8% to 100%. ,,,− ,− ,− ,, Many studies reported that the fatty acids C18:1 and C18:2 are the main components found in the composition of GTW. ,,,− ,,,, Table provides some characteristics of GTW oils obtained from previous studies.…”

Section: Lipid Extraction Processesmentioning

confidence: 99%

Production of Biodiesel from Recycled Grease Trap Waste: A Review

Tran

Tišma

Budžaki

et al. 2021

Ind. Eng. Chem. Res.

View full text Add to dashboard Cite

In recent years, climate changes occurring worldwide have encouraged research into the development of eco-friendly, lowerenvironmental-impact, and more-sustainable energy sources. This may also be a solution to deal with the rapid decrease in, and depletion of, fossil resources. Biodiesel has been considered as a key attribute for effective climate change responses and a potential candidate to substitute for mineral diesel. Even so, the marketability of biodiesel is still very limited, because of the lower price of fossil diesel, as well as the expensive cost of present biodiesel feedstocks. In this regard, the use of wastewater residuals such as scum sludge and grease trap waste (GTW) as a cost-effective feedstock appears to be attractive. Many studies have been conducted to investigate the characteristics of GTW in order to develop an appropriate technique for biodiesel production from this low-quality and low-cost resource. However, most of these studies have only focused on a partial process, including the pretreatment of feedstock, the esterification, and the transesterification processes, without reporting a fully integrated process for biodiesel production from recycled trap grease. Therefore, this study aims to highlight recent achievements in the production of biodiesel from GTW and to consider the potential marketability of this fuel.

show abstract

Enzymatic Production of Biodiesel: Strategies to Overcome Methanol Inactivation

Lotti

Pleiss

Valero

et al. 2018

Biotechnology Journal

View full text Add to dashboard Cite

Lipase-catalyzed transesterification of triglycerides and alcohols to obtain biodiesel is an environmentally friendly and sustainable route for fuels production since, besides proceeding in mild reaction conditions, it allows for the use of low-cost feedstocks that contain water and free fatty acids, for example non-edible oils and waste oils. This review article reports recent advances in the field and focus in particular on a major issue in the enzymatic process, the inactivation of most lipases caused by methanol, the preferred acyl acceptor used for alcoholysis. The recent results about immobilization of enzymes on nano-materials and the use of whole-cell biocatalysts, as well as the use of cell-surface display technologies and metabolic engineering strategies for microbial production of biodiesel are described. It is discussed also insight into the effects of methanol on lipases obtained by modeling approaches and report on studies aimed at mining novel alcohol stable enzymes or at improving robustness in existing ones by protein engineering.

show abstract

High-yielding, one-pot, and green production of biodiesel from waste grease using wet cells of a recombinant Escherichia coli strain as catalyst

Cited by 10 publications

References 31 publications

Production of Natural 2-Phenylethanol from Glucose or Glycerol with CoupledEscherichia coliStrains Expressingl-Phenylalanine Biosynthesis Pathway and Artificial Biocascades

Production of Natural 2-Phenylethanol from Glucose or Glycerol with CoupledEscherichia coliStrains Expressingl-Phenylalanine Biosynthesis Pathway and Artificial Biocascades

Production of Biodiesel from Recycled Grease Trap Waste: A Review

Enzymatic Production of Biodiesel: Strategies to Overcome Methanol Inactivation

Contact Info

Product

Resources

About