High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain

Kahar, Prihardi; Tsuge, Takeharu; Taguchi, Kazunori; Doi, Yoshiharu

doi:10.1016/s0141-3910(03)00227-1

Cited by 283 publications

(170 citation statements)

References 19 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…PHA content of this result was lower than that of obtained from Ralstonia eutropha using soy been oil as a carbon source, i.e. 76 % CDW [8] and from P. oleovorans using octanoate as a carbon source, 1.e. 67 % CDW [9].…”

Section: Resultscontrasting

confidence: 64%

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM OLEIC ACID USING Pseudomonas putida PGA1 BY FED BATCH CULTURE

Marsudi

Tan

Gan

et al. 2010

MST

View full text Add to dashboard Cite

Bacterial polyhydroxyalkanoates (PHAs) are a class of polymers currently receiving much attention because of their potential as renewable and biodegradable plastics. A wide variety of bacteria has been reported to produce PHAs including Pseudomonas strains. These strains are known as versatile medium chain length PHAs (PHAs-mcl) producers using fatty acids as carbon source. Oleic acid was used to produce PHAs-mcl using Pseudomonas putida PGA 1 by continuous feeding of both nitrogen and carbon source, in a fed batch culture. During cell growth, PHAs also accumulated, indicating that PHA production in this organism is growth associated. Residual cell increased until the nitrogen source was depleted. At the end of fermentation, final cell concentration, PHA content, and productivity were 30.2 g/L, 44.8 % of cell dry weight, and 0.188 g/l/h, respectively.

show abstract

Section: Resultscontrasting

confidence: 64%

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM OLEIC ACID USING Pseudomonas putida PGA1 BY FED BATCH CULTURE

Marsudi

Tan

Gan

et al. 2010

MST

View full text Add to dashboard Cite

show abstract

“…A. caviae is known to synthesize both SCL and MCL PHA containing four to six carbon atoms (Shimamura et al, 1994). Various plant oils including palm oil products have been used to produce P(3HB-co-3HHx) copolymer from the recombinant C. necator PHBˉ4 harboring the PHA synthase gene of A. caviae (Fukui and Doi, 1998;Kahar et al, 2004;Loo et al, 2005). The generation of a new monomer in a different host could be associated with the occurrence and substrate specificity of enzymes.…”

Section: Carbon Sourcesmentioning

confidence: 99%

Cloning and expression of the PHA synthase gene from a locally isolated Chromobacterium sp. USM2

Bhubalan¹,

Kam²,

Yong³

et al. 2010

MJM

View full text Add to dashboard Cite

Chromobacterium sp. USM2, a locally isolated bacterium was found to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV) copolymer with high 3HV monomer composition. The PHA synthase gene was cloned and expressed in Cupriavidus necator PHBˉ4 to investigate the possibilities of incorporating other monomer. The recombinant successfully incorporated 3-hydroxyhexanoate (3HHx) monomer when fed with crude palm kernel oil (CPKO) as the sole carbon source. Approximately 63 ± 2 wt% of P(3HB-co-3HHx) copolymer with 4 mol% of 3HHx was synthesized from 5 g/L of oil after 48 h of cultivation. In addition, P(3HB-co-3HV-co-3HHx) terpolymer with 9 mol% 3HV and 4 mol% 3HHx could be synthesized with a mixture of CPKO and sodium valerate. The presence of 3HV and 3HHx monomers in the copolymer and terpolymer was further confirmed with + H-NMR analysis. This locally isolated PHA synthase has demonstrated its ability to synthesize P(3HB-co-3HHx) copolymer from a readily available and renewable carbon source; CPKO, without the addition of 3HHx precursors.

show abstract

“…As a result, many studies have focused on exploiting microbial fatty acid degradation pathways in order to produce P(HB-co-HHx) (Budde et al 2011a, b;Khanna and Srivastava 2005). Indeed, previous reports have shown P(HBco-HHx), containing greater than 20 % content of 3-hydroxyhexanoate (3HHx) monomer, can be produced using plant oils as the sole carbon source (Budde et al 2011b;Kahara et al 2004;Riedel et al 2012). Although its biosynthetic pathway from butyrate through butyryl-CoA, 3-ketohexanoyl-CoA, and 3-hydroxyhexanoyl CoA to PHA can be predicted in butyrate metabolism (Fig.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha

Jeon

Brigham

Kim

et al. 2014

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHAs), a promising family of bio-based polymers, are considered to be alternatives to traditional petroleum-based plastics. Copolymers like poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) have been shown to exhibit favorable physical and mechanical properties, due to decreased crystallinity resulting from the presence of medium-chain-length 3-hydroxyhexanoate (3HHx) monomers. In this study, we produced P(HB-coHHx) using engineered Ralstonia eutropha strains containing deletions of the acetoacetyl-CoA reductase (phaB) genes and replacing the native PHA synthase with phaC2 from Rhodococcus aetherivorans I24 and by using butyrate, a short-chain organic acid, as the carbon source. Although the wild-type R. eutropha did not produce P(HB-co-HHx) when grown on mixed acids or on butyrate as the sole carbon source, we are able to produce polymer containing up to 40 wt% 3HHx monomer with the aforementioned engineered R. eutropha strains using various concentrations of just butyrate as the sole carbon source. This is the first report for the production of P(HB-co-HHx) copolymer in R. eutropha using butyrate.

show abstract

High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain

Cited by 283 publications

References 19 publications

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM OLEIC ACID USING Pseudomonas putida PGA1 BY FED BATCH CULTURE

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM OLEIC ACID USING Pseudomonas putida PGA1 BY FED BATCH CULTURE

Cloning and expression of the PHA synthase gene from a locally isolated Chromobacterium sp. USM2

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha

Contact Info

Product

Resources

About