High-Yield Preparation of Isolated Rat Liver Parenchymal Cells

Berry, Michael N.; Friend, Daniel S.

doi:10.1083/jcb.43.3.506

Cited by 4,294 publications

(1,004 citation statements)

References 35 publications

Supporting

Mentioning

970

Contrasting

Unclassified

Order By: Relevance

“…1,27,28 The rats were obtained from the local animal facility of the university, and all animals received care according to the German Animal Welfare Act. Cell culture techniques and the procedures of semiquantitative and quantitative reverse transcription polymerase chain reaction (RT-PCR) as well as western blot and immunofluorescence staining techniques were carried out as described.…”

Section: Methodsmentioning

confidence: 99%

The niche of stellate cells within rat liver

et al. 2009

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

The niche of stellate cells within rat liver

et al. 2009

View full text Add to dashboard Cite

“…25 The isolated cells were suspended in culture medium (75% Eagle's MEM and 25% Waymouth's medium supplemented with 10% FCS, 2 mm l-glutamine, 4 g/ml dexamethasone, 10 ng/ml T 3 (Sigma Chemical, St Louis, MO, USA), 50 ng/ml epidermal growth factor (Sigma) and ITS Universal Culture Supplement (Collaborative Biomedical Products, Bedford, MA, USA). The cells were kept on ice for immediate use or were plated on either collagen-coated 24-well dishes at a density of 2 × 10 5 cells per well or collagen-coated 96-well plates at density of 7500 cells per well.…”

Section: Methodsmentioning

confidence: 99%

An interaction between penton base and αv integrins plays a minimal role in adenovirus-mediated gene transfer to hepatocytes in vitro and in vivo

et al. 1998

View full text Add to dashboard Cite

Studies in cultured cell lines have shown that adenovirus tion of hepatocytes, even though the mutation impaired infection involves binding of adenovirus fiber to its cell surinfection of HeLa cells. Hepatocytes had undetectable face receptor and binding of penton base to ␣v integrins.amounts of ␣v integrins on their cell surface and showed However, much less is known about the role of these interno specific adherence to vitronectin, the natural substrate actions in cells that are targets for adenovirus-mediated of ␣v integrins. Adenovirus with an intact penton base gene transfer. Earlier work showed that hepatocytes are enhanced infection of liver following intravenous injection, readily infected by adenovirus, making them an attractive but only by three-fold as compared with virus in which the target for gene therapy in several diseases. We found that integrin-binding motif was disrupted. These studies sugaddition of fiber protein blocked adenovirus infection of prigest that interactions between cell surface integrins and mary cultures of hepatocytes. This suggests an important penton base are not required for adenovirus infection of role for fiber and its receptor. However, mutation of the hepatocytes in vitro, but the interaction enhances infection integrin-binding motif in penton base did not inhibit infecto a small degree in vivo.

show abstract

“…In vitro hepatocyte studies have largely been limited to primary cultures of hepatocytes established after collagenase perfusion of rat or mouse livers (Williams et al, 1977;Berry and Friend, 1969). These techniques were also amenable to the establishment of primary hepatocyte cultures in nonrodent species such as the pig (Caperna et al, 1985).…”

Section: Introductionmentioning

confidence: 99%