High yield preparation of ganglioside GM1 using recombinant sialidase from Cellulosimicrobium cellulans

Yuan, Ye; Li, Ji; Hu, Yanbo; Hu, Chenxing; Chen, Honglei; Gao, Juan; Zhou, Yifa

doi:10.1016/j.procbio.2017.04.010

Cited by 4 publications

(6 citation statements)

References 29 publications

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“…As sialidase CcSia was efficient in terms of reaction rate and GM1 production [27], CcSia was selected for immobilization studies. In this study, four kinds of particles were prepared, and sialidase was immobilized onto these supports with different methods.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we used the response surface methodology to optimize GM1 production conditions. The optimum conditions for a 2 h transformation were found to be a temperature of 32.5 °C and a pH of 5.2 [27]. Under the same reaction conditions, GM1 was prepared by transforming the extracted GLS with immobilized enzyme in this work.…”

Section: Resultsmentioning

confidence: 99%

“…Sialidases (neuraminidases, EC 3.2.1.18) are widely distributed in living organisms and participate in the metabolism of sialoglycoconjugates. Scientists have made more efforts to convert GLS to GM1 with sialidase-producing bacteria [22,23,24,25] or recombine sialidases [26,27]. The engineering of enzymes from biological entities to industrial reactors is a very exciting goal.…”

Section: Introductionmentioning

confidence: 99%

“…We got sialidase from Cellulosimicrobium cellulans sp. 21, which catalyzes the hydrolysis of α-2,3-, α-2,6-, and α-2,8-glycosidic linkages [25,27]. In this study, we established a method for extracting GLS from a fresh pig brain by SCE and chose an appropriate immobilization method to transform GLS into GM1.…”

Section: Introductionmentioning

confidence: 99%

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Preparation of Ganglioside GM1 by Supercritical CO2 Extraction and Immobilized Sialidase

Qiao

Zhang

et al. 2019

Molecules

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Monosialotetrahexosylganglioside (GM1) has good activity on brain diseases and was developed to be a drug applied in clinics for neurological disorders and nerve injury. It is difficult to isolate GM1 in industry scale from the brains directly. In this work, a simple and highly efficient method with high yield was developed for the isolation, conversion, and purification of GM1 from a pig brain. Gangliosides (GLS) were first extracted by supercritical CO2 (SCE). The optimum extraction time of GLS by SCE was 4 h, and the ratio of entrainer to acetone powder from the pig brain was 3:1 (v/w). GM1 was then prepared from GLS by immobilized sialidase and purified by reverse-phase silica gel. Sodium alginate embedding was used for the immobilization of sialidase. Under the optimized method, the yield of high-purity GM1 was around 0.056%. This method has the potential to be applied in the production of GM1 in the industry.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation of Ganglioside GM1 by Supercritical CO2 Extraction and Immobilized Sialidase

Qiao

Zhang

et al. 2019

Molecules

Self Cite

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“…1 g/L of recombinant Cellulosimicrobium sp. 21 sialidase, expressed in E. coil BL21 (DE3), converted 10 g/L of gangliosides with a yield of 52 % GM1 in a 200 mL medium . However, the GM1 production from gangliosides through the sialidase hydrolysis‐producing sialic acids as a by‐product showed inhibited the activity of the sialidase .…”

Section: Introductionmentioning

confidence: 99%

Enhancement of bioconversion gangliosides to monosialotetrahexosylganglioside by in situ sialic acid removal and recovery

2019

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Monosialotetrahexosylganglioside (GM1) production via bioconversion from gangliosides is promising for industrial application because it has the advantages of a high GM1 yield and an environmentally friendly process. Sialidase hydrolyzes gangliosides to GM1 producing sialic acid as a by-product, which inhibits the sialidase activity, while the incomplete conversion of gangliosides was indicated by thin-layer chromatography (TLC) in the presence of sialic acid. The sialic acid showed competitive inhibition on the sialidase activity with an inhibition constant of 0.75 mmol/L. By harnessing the in situ product removal (ISPR) technique, 50 g/L of crude gangliosides was completely converted to GM1 after a 12 h conversion. The GM1 concentration increased from 0.42 to 10.88 g/L in the ISPR system, which was 59.1 % higher than that of the control (6.84 g/L GM1). In addition, sialic acid was recovered simultaneously with a yield of 74.7 %. In summary, the ISPR system improved the bioconversion from gangliosides to GM1 and recovered sialic acid within a one-step bioprocess.

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Production optimization, purification, expression, and characterization of a novel α-l-arabinofuranosidase from Paenibacillus polymyxa

Gao

Zhao

Zhang

et al. 2018

Electronic Journal of Biotechnology

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High yield preparation of ganglioside GM1 using recombinant sialidase from Cellulosimicrobium cellulans

Cited by 4 publications

References 29 publications

Preparation of Ganglioside GM1 by Supercritical CO2 Extraction and Immobilized Sialidase

Preparation of Ganglioside GM1 by Supercritical CO2 Extraction and Immobilized Sialidase

Enhancement of bioconversion gangliosides to monosialotetrahexosylganglioside by in situ sialic acid removal and recovery

Production optimization, purification, expression, and characterization of a novel α-l-arabinofuranosidase from Paenibacillus polymyxa

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