High-yield oleaginous fungi and high-value microbial lipid resources from Mucoromycota

Zhao, Heng; Lv, Meilin; Liu, Ze; Zhang, Mingzhe; Wang, Yaning; Ju, Xiao; Song, Zhen; Ren, Liying; Jia, Bisi; Qiao, Min; Liu, Xiaoyong

doi:10.1007/s12155-020-10219-3

Cited by 22 publications

(19 citation statements)

References 50 publications

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“…Among the mycobiota shared in the three plots (Table 3), some species of Mortierella that were found are reported in the literature as fungi of bioprospective interest. M. exigua has potential as an agent of bioremediation against heavy metals [75], while M. camargensis and M. amoeboidea can both accumulate high concentrations of arachidonic acid in prospective bioenergy production [76]. Moreover, M. amoeboidea has shown herbicidal activities [77].…”

Section: Discussionmentioning

confidence: 99%

The Mycobiota of High Altitude Pear Orchards Soil in Colombia

Nicola

Landínez-Torres

Zambuto

et al. 2021

Biology

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In Colombia, the cultivation of deciduous fruit trees such as pear is expanding for socio-economic reasons and is becoming more and more important for the local population. Since organized cultivation is slowly replacing sustenance cultivation, scientific information on the present agro-environment is needed to proceed in this change in an organic and environmentally friendly way. In particular, this study is an accurate description of the mycobiota present in the bulk soil of two different high altitude pear orchards in the Colombian Andes. The metabarcoding of soil samples allowed an in-depth analysis of the whole fungal community. The fungal assemblage was generally dominated by Ascomycota and secondly by Mortierellomycota. As observed in other studies in Colombia, the genus Mortierella was found to be especially abundant. The soil of the different pear orchards appeared to host quite different fungal communities according to the soil physico-chemical properties. The common mycobiota contained 35 fungal species, including several species of Mortierella, Humicola, Solicoccozyma and Exophiala. Moreover, most of the identified fungal species (79%) were recorded for the first time in Colombian soils, thus adding important information on soil biodiversity regarding both Colombia and pear orchards.

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Section: Discussionmentioning

confidence: 99%

The Mycobiota of High Altitude Pear Orchards Soil in Colombia

Nicola

Landínez-Torres

Zambuto

et al. 2021

Biology

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“…Species of Absidia synthesized important metabolites, such as α-galactosidase, laccase, chitosan, and fatty acids [ 57 , 58 , 59 , 60 ], and our results provide a basis for their applications. In addition to the five new species proposed in this paper, 51 species of Absidia have previously been described from all around the world.…”

Section: Discussionmentioning

confidence: 94%

Species Diversity and Ecological Habitat of Absidia (Cunninghamellaceae, Mucorales) with Emphasis on Five New Species from Forest and Grassland Soil in China

Zhao

Nie

Zong

et al. 2022

JoF

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Although species of Absidia are known to be ubiquitous in soil, animal dung, and insect and plant debris, the species diversity of the genus and their ecological habitats have not been sufficiently investigated. In this study, we describe five new species of Absidia from forest and grassland soils in southwestern China, with support provided by phylogenetic, morphological, and physiological evidence. The species diversity and ecological habitat of Absidia are summarized. Currently, 22 species are recorded in China, which mainly occur in soil, especially in tropical and subtropical forests and mountains. An updated key to the species of Absidia in China is also provided herein. This is the first overview of the Absidia ecological habitat.

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“…Six samples (CK20, One20, Two20, CK80, One80, and Two80) of 0.05 g of urediniospores were used to detect concentrations of H 2 O 2 according to the protocol of Kit YX-W-A400 (Sinobest Bio Co., Ltd., Shanghai, China). In addition, 0.1 g of urediniospores from Two80 and 0.1 g from CK80 were selected to detect fatty acids using gas chromatography-mass spectrometry (GC-MS, QP2010; Shimadzu Corp., Kyoto, Japan) (Zhao et al, 2020). Each sample was biologically repeated three times, and the means of each detection were determined using Duncan's multiple range test (software SPSS 22.0, Chicago, IL, USA).…”

Section: Hydrogen Peroxide and Fatty Acid Detectionmentioning

confidence: 99%

Multinuclei Occurred Under Cryopreservation and Enhanced the Pathogenicity of Melampsora larici-populina

Zheng

Peng

et al. 2021

Front. Microbiol.

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Melampsora larici-populina is a macrocyclic rust, and the haploid stage with two nuclei and the diploid of mononuclear sequentially occur annually. During the preservation of dry urediniospores at −80°C, we found that one isolate, ΔTs06, was different from the usual wild-type isolate Ts06 at −20°C because it has mixed polykaryotic urediniospores. However, the other spores, including the 0, I, III, and IV stages of a life cycle, were the same as Ts06. After five generations of successive inoculation and harvest of urediniospores from the compatible host Populus purdomii, the isolate ΔTs06 steadily maintained more than 20% multiple nucleus spores. To test the pathogenesis variation of ΔTs06, an assay of host poplars was applied to evaluate the differences between ΔTs06 and Ts06. After ΔTs06 and Ts06 inoculation, leaves of P. purdomii were used to detect the expression of small secreted proteins (SSPs) and fungal biomasses using quantitative real-time PCR (qRT-PCR) and trypan blue staining. ΔTs06 displayed stronger expression of five SSPs and had a shorter latent period, a higher density of uredinia, and higher DNA mass. A transcriptomic comparison between ΔTs06 and Ts06 revealed that 3,224 were differentially expressed genes (DEGs), 55 of which were related to reactive oxygen species metabolism, the Mitogen-activated protein kinase (MAPK) signaling pathway, and the meiosis pathway. Ten genes in the mitotic and meiotic pathways and another two genes associated with the “response to DNA damage stimulus” all had an upward expression, which were detected by qRT-PCR in ΔTs06 during cryopreservation. Gas chromatography–mass spectrometry (GC-MS) confirmed that the amounts of hexadecanoic acid and octadecadienoic acid were much more in ΔTs06 than in Ts06. In addition, using spectrophotometry, hydrogen peroxide (H2O2) was also present in greater quantities in ΔTs06 compared with those found in Ts06. Increased fatty acids metabolism could prevent damage to urediniospores in super-low temperatures, but oxidant species that involved H2O2 may destroy tube proteins of mitosis and meiosis, which could cause abnormal nuclear division and lead to multinucleation, which has a different genotype. Therefore, the multinuclear isolate is different from the wild-type isolate in terms of phenotype and genotype; this multinucleation phenomenon in urediniospores improves the pathogenesis and environmental fitness of M. larici-populina.

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High-yield oleaginous fungi and high-value microbial lipid resources from Mucoromycota

Cited by 22 publications

References 50 publications

The Mycobiota of High Altitude Pear Orchards Soil in Colombia

The Mycobiota of High Altitude Pear Orchards Soil in Colombia

Species Diversity and Ecological Habitat of Absidia (Cunninghamellaceae, Mucorales) with Emphasis on Five New Species from Forest and Grassland Soil in China

Multinuclei Occurred Under Cryopreservation and Enhanced the Pathogenicity of Melampsora larici-populina

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