High‐Yield of Nucleic Acid Adsorption via Poly(Vinyl Alcohol‐<i>co</i>‐Ethylene) Nanofiber‐Based Anion‐Exchange Chitosan Aerogel Membrane with Controllable Porosity

Xu, Jia; Wang, Jing; Wan, Yucai; Cheng, Peng; Geng, Yun; Xia, Ming; Zheng, Luping; Tan, Yan; Liu, Ke; Wang, Dong

doi:10.1002/admi.202200613

Cited by 6 publications

(3 citation statements)

References 54 publications

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“…The EVOH nanofibers were generated by melt extrusion and phase separation, which have been proven to construct nanofibers [ 26 , 27 ]. An amount of 1 g of obtained EVOH nanofibers was dispersed into 100 mL of mixture solvent (deionized water and tert-butanol at a volume ratio of 3:1) by homogenization to form EVOH nanofibrous suspensions.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of EVOH/PANI Composite Nanofibrous Aerogels for the Removal of Dyes and Heavy Metal Ions

Jun-shan

Song

2023

Materials

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Water pollution caused by the leakage and discharge of pollutants, such as dyes and heavy metal ions, can cause serious damage to the environment and human health. Therefore, it is important to design and develop adsorbent materials that are efficient and multifunctional for the removal of these pollutants. In this work, poly(vinyl alcohol-co-ethylene) (EVOH)/polyaniline (PANI) composite nanofibrous aerogels (NFAs) were fabricated via solution oxidation and blending. The aerogels were characterized by a scanning electron microscope, Fourier transform infrared spectrometry, a contact angle measuring instrument and a universal testing machine. The influences of the introduction of PANI nanorods on the structural properties of aerogels were investigated, and the adsorption performance of aerogels was also studied. The results showed that the introduction of PANI nanorods filled the fibrous network structure, reduced porosity, increased surface hydrophilicity and improved compressive strength. Furthermore, EVOH/PANI composite NFAs possess good adsorption performances for dyes and heavy metal ions: The adsorption capacities of methyl orange and chromium ions (VI) are 73.22 mg/g and 115.54 mg/g, respectively. Overall, the research suggests that EVOH/PANI NFAs have great potential as efficient and multifunctional adsorbent materials for the removal of pollutants from water.

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Section: Methodsmentioning

confidence: 99%

Fabrication of EVOH/PANI Composite Nanofibrous Aerogels for the Removal of Dyes and Heavy Metal Ions

Jun-shan

Song

2023

Materials

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“…EVOH nanofibers were prepared using the melt-extrusion phase separation method, and these nanofibers were subsequently utilized as the fundamental components for the fabrication of aerogels [24][25][26]. In brief, a 1 gm sample of EVOH nanofiber was evenly dispersed in a 100 mL dispersion of tert-butanol and water.…”

Section: Preparation Of Evoh Nanofibrous Aerogels (Nfas)mentioning

confidence: 99%

Butane Tetracarboxylic Acid Grafted on Polymeric Nanofibrous Aerogels for Highly Efficient Protein Absorption and Separation

Lu,

Jiang,

Qiao

et al. 2024

Polymers

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Developing high-performance and low-cost protein purification materials is of great importance to meet the demands for highly purified proteins in biotechnological industries. Herein, a facile strategy was developed to design and construct high-efficiency protein absorption and separation media by combining aerogels’ molding techniques and impregnation processes. Poly (ethylene-co-vinyl alcohol) (EVOH) nanofibrous aerogels (NFAs) were modified by grafting butane tetracarboxylic acid (BTCA) over them in situ. This modification was carried out using polyphosphoric acid as a catalyst. The resulting EVOH/BTCA NFAs exhibited favorable comprehensive properties. Benefiting from the highly interconnected porous structure, good underwater compressive properties, and abundant absorption ligands, the obtained EVOH/BTCA NFAs possessed a high static absorption capacity of 1082.13 mg/g to lysozyme and a short absorption equilibrium time of about 6 h. A high saturated dynamic absorption capacity for lysozyme (716.85 mg/g) was also realized solely by gravity. Furthermore, EVOH/BTCA NFAs displayed excellent reusability, good acid and alkaline resistance, and unique absorption selectivity performance. The successful synthesis of such aerogels can provide a potential candidate for next-generation protein absorbents for bio-separation and purification engineering.

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“…In recent years, extraction-free techniques such as utilizing surface charge interactions between polymers and RNAs have become a promising approach for hassle-free RNA extraction. Xu et al successfully used poly(vinyl alcohol- co -ethylene) (EVOH) nanofiber-based polyethylenimine (PEI) chitosan aerogel (ENPC) membranes for nucleic acid extraction . Additionally, a poly(aminoester) thin film was used to cover magnetic nanoparticles (MNP) and extract COVID-19 RNA from nasopharyngeal swabs. , However, these methods often require high process temperatures (approximately 180–220 °C) to form functional polymer surfaces onto a wide range of substrates, hindering their application to vulnerable substrates to lower the extraction system’s cost.…”

mentioning

confidence: 99%

Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR

Choi,

Song,

Cho

et al. 2023

Anal. Chem.

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The ongoing coronavirus disease 2019 (COVID-19) pandemic demands rapid and straightforward diagnostic tools to prevent early-stage viral transmission. Although nasopharyngeal swabs are a widely used patient sample collection method for diagnosing COVID-19, using these samples for diagnosis without RNA extraction increases the risk of obtaining false-positive and -negative results. Thus, multiple purification steps are necessary, which are time-consuming, generate significant waste, and result in substantial sample loss. To address these issues, we developed surface-modified polymerase chain reaction (PCR) tubes using the tertiary aminated polymer poly(2-dimethylaminomethylstyrene) (pDMAMS) via initiated chemical vapor deposition. Introducing the clinical samples into the pDMAMS-coated tubes resulted in approximately 100% RNA capture efficiency within 25 min, which occurred through electrostatic interactions between the positively charged pDMAMS surface and the negatively charged RNA. The captured RNA is then detected via chamber digital PCR, enabling a sensitive, accurate, and rapid diagnosis. Our platform provides a simple and efficient RNA extraction and detection strategy that allows detection from 22 nasopharyngeal swabs and 21 saliva specimens with 0% false negatives. The proposed method can facilitate the diagnosis of COVID-19 and contribute to the prevention of early-stage transmission.

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High‐Yield of Nucleic Acid Adsorption via Poly(Vinyl Alcohol‐co‐Ethylene) Nanofiber‐Based Anion‐Exchange Chitosan Aerogel Membrane with Controllable Porosity

Cited by 6 publications

References 54 publications

Fabrication of EVOH/PANI Composite Nanofibrous Aerogels for the Removal of Dyes and Heavy Metal Ions

Fabrication of EVOH/PANI Composite Nanofibrous Aerogels for the Removal of Dyes and Heavy Metal Ions

Butane Tetracarboxylic Acid Grafted on Polymeric Nanofibrous Aerogels for Highly Efficient Protein Absorption and Separation

Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR

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