High Yield of Cells Committed to the Photoreceptor Fate from Expanded Mouse Retinal Stem Cells

Merhi-Soussi, Faten; Angénieux, Brigitte; Canola, Kriss; Kostic, Corinne; Tekaya, M.; Hornfeld, D.; Arsenijévic, Yvan

doi:10.1634/stemcells.2005-0311

Cited by 40 publications

(50 citation statements)

References 63 publications

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“…However, the addition of growth factors increases the number of spheres formed significantly (Coles et al 2004). Current culture methods mostly use EGF and/ or FGF-2 in the growth media at a concentration of 10-20 ng/ ml Coles et al 2004;Klassen et al 2004;Merhi-Soussi et al 2006;Tropepe et al 2000).…”

Section: In Vitro Propagation Of Rscsmentioning

confidence: 99%

“…These cells remain homogeneous in an undifferentiated state, even after extensive passaging, as shown by the expression of the progenitor markers NES, BMI1, Ascl1 Mash1 , and Pax6 and by the lack of differentiated cell markers, such as glial fibrillary acidic protein (GFAP) or beta-tubulin 3 ). These cells have a vigorous capacity to maintain their potential to generate neurons, even after extensive passaging (Merhi-Soussi et al 2006). Importantly, neural SCs isolated from brain radial glia have similar characteristics of expansion and robust differentiation (Conti et al 2005).…”

Section: In Vitro Propagation Of Rscsmentioning

confidence: 99%

“…Interestingly, we have observed a number of neurons expressing RGC markers confirming that late RSCs can generate early neurons. A more extensive analysis with the neonatal RSCs derived from the radial glia population has demonstrated their potential to differentiate into the photoreceptor lineage (Merhi-Soussi et al 2006). However, whether a single neonatal RSC is able to generate all retinal cell types in vitro remains to be determined.…”

Section: Differentiation Of Retinal Cells In Vitromentioning

confidence: 99%

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Retinal stem cells: promising candidates for retina transplantation

Djojosubroto

Arsenijévic

2007

Cell Tissue Res

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Stem cell transplantation is widely considered as a promising therapeutic approach for photoreceptor degeneration, one of the major causes of blindness. In this review, we focus on the biology of retinal stem cells (RSCs) and progenitor cells (RPCs) isolated from fetal, postnatal, and adult animals, with emphasis on those from rodents and humans. We discuss the origin of RSCs/RPCs, the markers expressed by these cells and the conditions for the isolation, culture, and differentiation of these cells in vitro or in vivo by induction with exogenous stimulation.

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Section: In Vitro Propagation Of Rscsmentioning

confidence: 99%

Section: In Vitro Propagation Of Rscsmentioning

confidence: 99%

Section: Differentiation Of Retinal Cells In Vitromentioning

confidence: 99%

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Retinal stem cells: promising candidates for retina transplantation

Djojosubroto

Arsenijévic

2007

Cell Tissue Res

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“…These cells can extensively replicate [15,16], are able to form glia and neurons in vitro and depending on the protocols can generate neurons expressing some retinal markers [15][16][17]. After transplantation of non-differentiated expanded retinal progenitors in different animal models, these cells tend to form glia and some rare neurons with retinal characteristics [16][17][18][19]. Cells expressing some photoreceptor markers were reported, but these transplanted cells never display a convincing complete maturation with the presence of well-formed outer segments and synapses with the target cells, the bipolar cells.…”

Section: Introductionmentioning

confidence: 99%

“…The exposure in vitro of retinal stem cell-like cells to different factors known to participate to photoreceptor development commits the non-differentiated cells towards a retinal neuron fate [16,17] including the photoreceptor fate. However, these cells express only few specific retinal markers and don't robustly integrate into the retina after cell transplantation [16,18,21].…”

Section: Introductionmentioning

confidence: 99%

An Advocacy for the Use of 3D Stem Cell Culture Systems for the Development of Regenerative Medicine: An Emphasis on Photoreceptor Generation

Decembrini¹

2015

J Stem Cell Res Ther

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IntroductionThe possibility to grow in vitro a specific cell population brings a broad potential to study the biological characteristics of the cell of interest. A culture system allows to dissect the intrinsic processes controlling different cell functions and to investigate cell interactions. This knowledge will serve as a standard to study pathophysiological mechanisms as well as to evaluate the potential of the cells of interest to become a tool for regenerative medicine, for tissue repair using cell transplantation or cell mobilization in situ for cell replacement. Successes were achieved for epithelial cells of the skin [1] or the cornea [2], but innovative protocols have to be established for many organs with a complex structure.The large diversity of the cell populations composing the central nervous system brings a new challenge to the generation of specific neuronal phenotypes. Several groups have attempted and continue to strive to generate fully differentiated neurons in a culture dish such as dopaminergic neurons, motoneurons, serotoninergic neurons and photoreceptors for instance. In this review, we will focus on the advances made in neuroscience that have favored the strategic development of new technologies to drive the generation of specific CNS neuron phenotypes and on photoreceptor production from stem cells.One gold standard, to categorize a cell identity or to evaluate the efficacy of cell commitment from stem cells, is the transplantation challenge to determine whether the isolated cell phenotype can integrate its population in vivo and participate to a physiological function, the pioneer field using this approach being immunology [3]. Concerning the CNS, homotopic transplantation of neural cells attested that certain cell populations can integrate into their original brain region in a host AbstractThe availability of stem cells is of great promise to study early developmental stages and to generate adequate cells for cell transfer therapies. Although many researchers using stem cells were successful in dissecting intrinsic and extrinsic mechanisms and in generating specific cell phenotypes, few of the stem cells or the differentiated cells show the capacity to repair a tissue. Advances in cell and stem cell cultivation during the last years made tremendous progress in the generation of bona fide differentiated cells able to integrate into a tissue after transplantation, opening new perspectives for developmental biology studies and for regenerative medicine. In this review, we focus on the main works attempting to create in vitro conditions mimicking the natural environment of CNS structures such as the neural tube and its development in different brain region areas including the optic cup. The use of protocols growing cells in 3D organoids is a key strategy to produce cells resembling endogenous ones. An emphasis on the generation of retina tissue and photoreceptor cells is provided to highlight the promising developments in this field. Other examples are presented and discussed, such as ...

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Retinal Stem Cells and Regeneration of Vision System

Yip

2013

The Anatomical Record

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The vertebrate retina is a well-characterized model for studying neurogenesis. Retinal neurons and glia are generated in a conserved order from a pool of mutlipotent progenitor cells. During retinal development, retinal stem/progenitor cells (RPC) change their competency over time under the influence of intrinsic (such as transcriptional factors) and extrinsic factors (such as growth factors). In this review, we summarize the roles of these factors, together with the understanding of the signaling pathways that regulate eye development. The information about the interactions between intrinsic and extrinsic factors for retinal cell fate specification is useful to regenerate specific retinal neurons from RPCs. Recent studies have identified RPCs in the retina, which may have important implications in health and disease. Despite the recent advances in stem cell biology, our understanding of many aspects of RPCs in the eye remains limited. PRCs are present in the developing eye of all vertebrates and remain active in lower vertebrates throughout life. In mammals, however, PRCs are quiescent and exhibit very little activity and thus have low capacity for retinal regeneration. A number of different cellular sources of RPCs have been identified in the vertebrate retina. These include PRCs at the retinal margin, pigmented cells in the ciliary body, iris, and retinal pigment epithelium, and M€ uller cells within the retina. Because PRCs can be isolated and expanded from immature and mature eyes, it is possible now to study these cells in culture and after transplantation in the degenerated retinal tissue. We also examine current knowledge of intrinsic RPCs, and human embryonic stems and induced pluripotent stem cells as potential sources for cell transplant therapy to regenerate the diseased retina. Anat Rec, 297:137-160,

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High Yield of Cells Committed to the Photoreceptor Fate from Expanded Mouse Retinal Stem Cells

Cited by 40 publications

References 63 publications

Retinal stem cells: promising candidates for retina transplantation

Retinal stem cells: promising candidates for retina transplantation

An Advocacy for the Use of 3D Stem Cell Culture Systems for the Development of Regenerative Medicine: An Emphasis on Photoreceptor Generation

Retinal Stem Cells and Regeneration of Vision System

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