High-yield expression of extracellular lipase from Yarrowia lipolytica and its interactions with lipopeptide biosurfactants: A biophysical approach

Janek, Tomasz; Mirończuk, Aleksandra M.; Rymowicz, Waldemar; Dobrowolski, Adam

doi:10.1016/j.abb.2020.108475

Cited by 21 publications

(7 citation statements)

References 43 publications

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“…A significant increase in CRL activity (82% compared to control) was also observed upon addition of 40 mg/L viscosinamide. Janek et al 17 showed that viscosinamide and amphisin have effects on the activity of lipase from Yarrowia lipolytica , suggesting that lipopeptide biosurfactants, as natural surfactants, have the ability to open the conformation of lipases. As a result, it is believed that viscosinamide creates an expedient environment for the substrate to link and interact with the active site of the lipase.…”

Section: Resultsmentioning

confidence: 99%

“…Lipases are amphiphilic and the association with other amphiphilic substances, such as biosurfactants, is expected to occur 47 . This binding is complex and depends strongly on many different aspects, such as the nature of both species and of the solvent, concentrations, etc.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The activity of lipases can be enhanced by the presence of biosurfactants 17 . Several studies have described the interactions between lipases and synthetic or microbial surfactants 17 – 19 . Lipases (triacylglycerol hydrolases, EC 3.1.1.3) catalyze the hydrolysis of triacylglycerols to glycerol and long-chain fatty acids 20 .…”

Section: Introductionmentioning

confidence: 99%

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Bioconversion of waste glycerol into viscosinamide by Pseudomonas fluorescens DR54 and its activity evaluation

Jama,

Łaba,

Kruszelnicki

et al. 2024

Sci Rep

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Lipopeptides, derived from microorganisms, are promising surface-active compounds known as biosurfactants. However, the high production costs of biosurfactants, associated with expensive culture media and purification processes, limit widespread industrial application. To enhance the sustainability of biosurfactant production, researchers have explored cost-effective substrates. In this study, crude glycerol was evaluated as a promising and economical carbon source in viscosinamide production by Pseudomonas fluorescens DR54. Optimization studies using the Box − Behnken design and response surface methodology were performed. Optimal conditions for viscosinamide production including glycerol 70.8 g/L, leucine 2.7 g/L, phosphate 3.7 g/L, and urea 9.3 g/L were identified. Yield of viscosinamide production, performed under optimal conditions, reached 7.18 ± 0.17 g/L. Preliminary characterization of viscosinamide involved the measurement of surface tension. The critical micelle concentration of lipopeptide was determined to be 5 mg/L. Furthermore, the interactions between the viscosinamide and lipase from Candida rugosa (CRL) were investigated by evaluating the impact of viscosinamide on lipase activity and measuring circular dichroism. It was observed that the α-helicity of CRL increases with increasing viscosinamide concentration, while the random coil structure decreases.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioconversion of waste glycerol into viscosinamide by Pseudomonas fluorescens DR54 and its activity evaluation

Jama,

Łaba,

Kruszelnicki

et al. 2024

Sci Rep

Self Cite

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“…Expression systems featuring Yarrowia and Hansenula are also commonly used for recombinant lipase expression; Yarrowia have been used to promote overexpression of their own lipase ( Janek et al, 2020 ).…”

Section: Heterologous Expression and Molecular Modification Of Microbial Lipasesmentioning

confidence: 99%

A Valuable Product of Microbial Cell Factories: Microbial Lipase

Yao

Liu

et al. 2021

Front. Microbiol.

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As a powerful factory, microbial cells produce a variety of enzymes, such as lipase. Lipase has a wide range of actions and participates in multiple reactions, and they can catalyze the hydrolysis of triacylglycerol into its component free fatty acids and glycerol backbone. Lipase exists widely in nature, most prominently in plants, animals and microorganisms, among which microorganisms are the most important source of lipase. Microbial lipases have been adapted for numerous industrial applications due to their substrate specificity, heterogeneous patterns of expression and versatility (i.e., capacity to catalyze reactions at the extremes of pH and temperature as well as in the presence of metal ions and organic solvents). Now they have been introduced into applications involving the production and processing of food, pharmaceutics, paper making, detergents, biodiesel fuels, and so on. In this mini-review, we will focus on the most up-to-date research on microbial lipases and their commercial and industrial applications. We will also discuss and predict future applications of these important technologies.

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