High yield bio-butanol production by solvent-producing bacterial microflora

Cheng, Chieh-Lun; Che, Pei-Yi; Chen, Bor-Yann; Lee, Wen-Jhy; Chien, Liang-Jung; Chang, Jo‐Shu

doi:10.1016/j.biortech.2011.12.133

Cited by 85 publications

(35 citation statements)

References 28 publications

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“…Amounts of acetic acid and butyric acid were observed to be low (1.6 $ 2.1 g/L) in fermentation broths (data not shown), suggesting that the metabolic pattern was shifted toward more reduced metabolites as reflected by higher butanol-to-acid ratio (1.5-2.0). Unlike engineered butanologenic Escherichia coli and C. sporogenes BE01, strain MF28 produced butanol continuously and did not exhibit obvious acidogenic and solventogenic phase during the entire fermentation process (Chen et al, 2013a,b;Gottumukkala et al, 2013;Cheng et al, 2012). In addition, strain MF 28 did not exhibit any carbon catabolite repression.…”

Section: Identification and Characterization Of A Mesophilic Strain Mmentioning

confidence: 99%

Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

2016

Bioresource Technology

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h i g h l i g h t sSimultaneous saccharification and fermentation of xylan to butanol by new strain MF28. Simultaneous fermentation of glucose and xylose to produce 11.9 g/L butanol. High yields of butanol on xylan and xylooligosaccharide were obtained. No acetone/ethanol byproducts were formed in converting lignocellulose to butanol. Non-sporulating strain MF28 is capable of continuous industrial-scale fermentation. a r t i c l e i n f o t r a c tProduction of lignocellulosic butanol has drawn increasing attention. However, currently few microorganisms can produce biofuels, particularly butanol, from lignocellulosic biomass via simultaneous saccharification and fermentation. Here we report discovery of a wild-type, mesophilic Clostridium sp. strain MF28 that ferments xylan to produce butanol (up to 3.2 g/L) without the addition of saccharolytic enzymes and without any chemical pretreatments. Application of selective pressure from 2-deoxy-Dglucose facilitated isolation of strain MF28, which exhibits inactivation of genes (gid and ccp genes) responsible for carbon catabolite repression, thus allowing strain MF28 to simultaneously ferment a combination of glucose (30 g/L), xylose (15 g/L), and arabinose (15 g/L) to produce 11.9 g/L of butanol. Strain MF28 possesses several unique features: (i) non-sporulating, (ii) no acetone/ethanol, (iii) complete hemicellulose-binding enzymatic domain, and (iv) absence of carbon catabolite repression. These unique characteristics demonstrate the industrial potential of strain MF28 for cost-effective biofuel generation from lignocellulosic biomass.

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Section: Identification and Characterization Of A Mesophilic Strain Mmentioning

confidence: 99%

Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

2016

Bioresource Technology

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“…Furthermore, Clostridium acetobutylicum was the first bacterium used for ABE fermentation [2]. However, other clostridium sp.…”

Section: Microorganismmentioning

confidence: 99%

“…In general, fermentation using Clostridia sp. results in the ABE production of around 15-25 g/L with a yield of 0.25-0.4 g ABE/g sugar [2]. Substrate inhibition has not been the major concern in ABE fermentation when glucose is used as a carbon source [53].…”

Section: Microorganismmentioning

confidence: 99%

“…Reasons for this trend include growing concerns about global warming and climatic change, volatility of oil supply, increasing price of crude oil and legislation restricting the use of nonrenewable energy sources. Furthermore, the generation of biofuels may improve the local employment opportunities and contribute to the reduction of CO 2 emissions [1][2][3]. Among the alternative fuels, biobutanol has shown promise as its properties are similar to gasoline [4] and, in comparison with ethanol, it has a longer carbon chain length as well as higher volatility, polarity, combustion value, octane rating [5] and is less corrosive [6].…”

Section: Introductionmentioning

confidence: 99%

“…Commercial butanol fermentation processes have been developed by some companies [2]. There is an expectation that the number of companies devoted to biobutanol production will increase worldwide as well as the development of new technologies to increase the yield [9].…”

Section: Introductionmentioning

confidence: 99%

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Recent advances on biobutanol production

Visioli

Enzweiler

Kuhn

et al. 2014

Sustain Chem Process

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Recent studies have shown that butanol is a potential gasoline replacement that can also be blended in significant quantities with conventional diesel fuel. However, biotechnological production of butanol has some challenges such as low butanol titer, high cost feedstocks and product inhibition. The present work reviewed the technical and economic feasibility of the main technologies available to produce biobutanol. The latest studies integrating continuous fermentation processes with efficient product recovery and the use of mathematical models as tools for process scale-up, optimization and control are presented.

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