High Xylose Yields from Dilute Acid Pretreatment of Corn Stover Under Process-Relevant Conditions

Weiss, Noah D.; Nagle, Nick; Tucker, Melvin P.; Elander, Richard T.

doi:10.1007/s12010-008-8490-y

Cited by 40 publications

(20 citation statements)

References 25 publications

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“…When side chains of acetyl group present in hemicellulose are released, acetic acid is generated. Various researchers reported a range of 1.9-7.3% acetyl group for corn stover (Balan et al, 2009;Kim & Lee, 2005b;Torget et al, 1991;Weiss et al, 2009). Acetic acid was the only byproduct found in most of the pretreated corn stover samples in the range of 0.060-0.168 g/L.…”

Section: Byproducts Formationmentioning

confidence: 98%

Application of Response Surface Methodology to Optimize Alkali Concentration, Corn Stover Particle Size, and Extruder Parameters for Maximum Sugar Recovery

Karunanithy¹,

Muthukumarappan²

2011

Biofuel Production-Recent Developments and Prospects

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Section: Byproducts Formationmentioning

confidence: 98%

Application of Response Surface Methodology to Optimize Alkali Concentration, Corn Stover Particle Size, and Extruder Parameters for Maximum Sugar Recovery

Karunanithy¹,

Muthukumarappan²

2011

Biofuel Production-Recent Developments and Prospects

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“…Dilute-acid pretreatment has proven to be a robust means of converting herbaceous lignocellulosic feedstock to fermentable 5-and 6-carbon sugars [1]. However, it is also prone to producing potent fermentation inhibitors (i.e., furfural), and also releases acetic acid from the acetyl groups present in biomass, commingled with the desired sugars.…”

Section: Introductionmentioning

confidence: 99%

“…All authors read and approved the final manuscript. 1 Transportation and Hydrogen Systems Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80004, USA.…”

mentioning

confidence: 99%

Development and characterization of a high-solids deacetylation process

Shekiro

Chen

Smith

et al. 2016

Sustain Chem Process

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Background: Dilute-acid pretreatment has proven to be a robust means of converting herbaceous feedstock to fermentable sugars. However, it also releases acetic acid, a known fermentation inhibitor, from acetyl groups present in the biomass. A mild, dilute alkaline extraction stage was implemented prior to acid pretreatment to separate acetic acid from the hydrolysate sugar stream. This step, termed deacetylation, improved the glucose and xylose yields from enzymatic hydrolysis and ethanol yields from fermentation of the sugars relative to the control experiments using dilute-acid pretreatment of native corn stover without deacetylation. While promising, deacetylation as it was historically practiced is conducted at low solids loadings, and at fixed conditions. Thus, many questions have been left unanswered, including the relationship between sodium hydroxide and solids loading, and acetate and xylan solubilization, as well as the impact of temperature and residence time on the process efficacy.Results: A central composite experiment was designed to evaluate the impact of solids loading, sodium hydroxide loading, reaction time and temperature during deacetylation on the acetate and xylan solubilization of corn stover. Using the ANOVA test, it became apparent that neither of the responses was significantly impacted by the solids loading, while the reaction time was a minor factor-the responses were largely driven by reaction temperature and the sodium hydroxide loading. Based on the results, we successfully demonstrated the ability to transition the low-solids (10 % w/w) deacetylation process to a higher-solids process (30 % w/w) with minimal impact on the ability to extract acetate from biomass. Conditions were selected to minimize xylose loss during deacetylation, while also removing 70 % of acetyl groups. The impact of selected conditions on the enzymatic hydrolysis and fermentation was further investigated. Conclusions:Evaluation of the whole-process impact demonstrated that despite the upfront reduction in carbohydrate loss during deacetylation, the overall process sugar yields were depressed by the high-solids, low alkali process relative to the historical control. Consequently, ethanol titers were reduced, though strong fermentation performance was still observed, indicating that 70 % acetate removal is sufficient to depress acetic acid concentrations to a level that does not substantially inhibit ethanol fermentation by rZymomonas.

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“…Furthermore, current promising pretreatment technologies are able to generate xylose-rich hydrolysates from corn stover, a model lignocellulosic feedstock [29][30][31]. Therefore, it is necessary to determine the feasibility and limits of SA production on a clean xylose feed to establish a baseline for lignocellulosic fermentations, and to gain insight into the extent to which C5 carbohydrates can be utilised by A. succinogenes.…”

Section: Introductionmentioning

confidence: 99%

Continuous succinic acid production from xylose by Actinobacillus succinogenes

Bradfield

Nicol

2015

Bioprocess Biosyst Eng

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) and carbohydrate consumption rates were similar. Also, mass balance closures on xylose were up to 18.2% lower than those on glucose. A modified HPLC method revealed pyruvic acid excretion at appreciable concentrations (1.2 -) which improved the mass balance closure by up to 16.8%. Furthermore, redox balances based on the accounted xylose consumed and the excreted metabolites, indicated an overproduction of reducing power. The oxidative pentose phosphate pathway was shown to be a plausible source of the additional reducing power.

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High Xylose Yields from Dilute Acid Pretreatment of Corn Stover Under Process-Relevant Conditions

Cited by 40 publications

References 25 publications

Application of Response Surface Methodology to Optimize Alkali Concentration, Corn Stover Particle Size, and Extruder Parameters for Maximum Sugar Recovery

Application of Response Surface Methodology to Optimize Alkali Concentration, Corn Stover Particle Size, and Extruder Parameters for Maximum Sugar Recovery

Development and characterization of a high-solids deacetylation process

Continuous succinic acid production from xylose by Actinobacillus succinogenes

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