High-volume pre-dilution on-line hemodiafiltration is the adequate blood purification method from the viewpoint of amino acid nutrition

Abstract

Background We compared amino acid leakage among high-volume pre-dilution on-line hemodiafiltration (Pre O-HDF), hemodialysis (HD), and post-dilution on-line hemodiafiltration (Post O-HDF). Subjects and methods The subjects were 9 patients. For HD, the total dialysate flow rate was established as 500 mL/min. For high-volume Pre O-HDF, it was established as 600 mL/min, under a replacement fluid volume of 90 L. For Post O-HDF, it was established as 600 mL/min, under a replacement fluid volume of 10 L. In both procedures, the duration of treatment was 4 h, and the blood flow volume was 200 mL/min. We compared the leakages of total amino acid, non-essential amino acid, and essential amino acid, clear spaces (CSs), β2-microglobulin (β2-MG) reduction rate, Kt/V for urea, and albumin leakage among the three procedures. Results Amino acid leakages after high-volume Pre O-HDF were significantly lower than HD and Post O-HDF. The CSs after high-volume Pre O-HDF were significantly lower than Post O-HDF. The β2-MG reduction rate after high-volume Pre O-HDF was significantly lower than Post O-HDF. The Kt/V for urea was not significantly different. Albumin leakages were below the detection limit (< 1 g) in the three procedures. Conclusion Under the treatment conditions we performed this time, high-volume Pre O-HDF reduces amino acid leakage in comparison with HD and Post O-HDF. High-volume Pre O-HDF is a therapeutic mode that suppresses amino acid leakage.

Study of the Metabolite Changes in Ganoderma lucidum under Pineapple Leaf Residue Stress via LC-MS/MS Coupled with a Non-Targeted Metabolomics Approach

“…With the vigorous development of national animal husbandry, the gap in protein-related feed was increasing, and amino acids such as lysine and histidine were essential amino acids for animal growth and development as well as being very scarce in animal feeding. Amino acids are closely related to animal metabolism, not only promoting animal growth but also improving feed utilization [35]. Among them, lysine not only participated in protein synthesis (including skeletal muscle, peptide hormones, plasma proteins, enzymes, and other key proteins), lipid metabolism, ketone body metabolism, and glucose production, but also regulated the expression of some genes in intestinal cells, which had an impact on the absorption and metabolism of other amino acids and further affected the absorption and metabolism of proteins.…”