High-Vigor Maize Seeds Resist Fusarium graminearum Infection through Stronger Ca2+ Signaling

Abstract

Seeds with high vigor have strong resistance to various adverse environmental conditions. However, little is known about how seed vigor affects the resistance of seeds to biotic stress. In this study, newly harvested seeds that had high vigor and seeds with low vigor, achieved via an artificially accelerated aging treatment, were used in the germination test after inoculation with Fusarium graminearum for 24 h. The results showed that high-vigor seed-related germination and seedling growth were not significantly affected by F. graminearum infection, while those related to low-vigor seeds were significantly inhibited. Analysis of transgenic maize seeds expressing the luminescent Ca2+ probe encoded by aequorin indicated that the concentration of free Ca2+ in the cytoplasm and nucleus of the embryo cells of high-vigor seeds was significantly higher than that of the low-vigor seeds. Through an experiment with Ca2+ inhibitor treatment and exogenous Ca2+ application, we further confirmed that Ca2+ played an important role in seed germination and seedling growth. Interestingly, in the presence of F. graminearum, the Ca2+ required for seed germination and seedling growth mainly came from the vacuolar calcium pool, while in the absence of F. graminearum, the required Ca2+ mainly came from the apoplastic calcium store. This study helps understand how high-vigor seeds resist disease and provides theoretical support for the wide application of high-vigor seeds in agricultural production.

Genome-Wide Identification and Expression Analysis of Rosa roxburghii Autophagy-Related Genes in Response to Top-Rot Disease

“…Next, it will transmit immune signals to downstream cellular responses through a decoding mechanism formed by Ca 2+ sensors [ 36 ]. In addition, the germination of highly viable maize seeds was not affected by F. graminearum infection because it probably had a higher concentration of free Ca 2+ in the cytoplasm of the embryonic cells [ 38 ]. Moreover, the dependent function of AtATG18a in the cytoplasm was sufficient to induce autophagy and enhance resistance against B. cinerea [ 34 ].…”

“…When Arabidopsis plants were grown in a low Ca 2+ medium, the reduction of PTI responses was examined [ 37 ]. Similarly, the high-vigor maize seeds grew better without being affected by pathogens due to the higher concentration of free Ca 2+ in the cytoplasm and nucleus [ 38 ]. Recently, the most prevalent approach to reducing the damage caused by various kinds of stresses was to increase intracellular Ca 2+ concentration through the application of exogenous calcium salt [ 39 ].…”