High Variations in Endospore Numbers within Tidal Flat Sediments Revealed by Quantification of Dipicolinic Acid

Fichtel, Jörg; Köster, Jürgen; Rullkötter, Jürgen; Sass, Henrik

doi:10.1080/01490450802402877

Cited by 16 publications

(12 citation statements)

References 32 publications

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“…However, sediment texture has been shown to affect endospore abundance in aquatic sediments, with significantly lower numbers found in sandy sediments compared to black muds with higher TOC (Fichtel et al ., ); this is different to what is known for biogeochemically active microbial communities which can reach high abundances in sandy sediments (de Beer et al ., ; Musat et al ., ). Fichtel and colleagues () suggested that endospores delivered from an external source, i.e., inactive dispersing propagules, are preferentially captured in muddy sediments with small pore space and lower hydraulic conductivity. Low TOC sandy sediment at station A may, therefore, retain fewer endospores as compared to other stations consisting of muddy sediments with higher TOC (Table ) and not necessarily be reflective of the numbers of endospores carried downstream.…”

Section: Discussioncontrasting

confidence: 79%

Distribution of thermophilic endospores in a temperate estuary indicate that dispersal history structures sediment microbial communities

Bell

Blake

Sherry

et al. 2018

Environmental Microbiology

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Endospores of thermophilic bacteria are found in cold and temperate sediments where they persist in a dormant state. As inactive endospores that cannot grow at the low ambient temperatures, they are akin to tracer particles in cold sediments, unaffected by factors normally governing microbial biogeography (e.g., selection, drift, mutation). This makes thermophilic endospores ideal model organisms for studying microbial biogeography since their spatial distribution can be directly related to their dispersal history. To assess dispersal histories of estuarine bacteria, thermophilic endospores were enriched from sediments along a freshwater-to-marine transect of the River Tyne in high temperature incubations (50°C). Dispersal histories for 75 different taxa indicated that the majority of estuarine endospores were of terrestrial origin; most closely related to bacteria from warm habitats associated with industrial activity. A subset of the taxa detected were marine derived, with close relatives from hot deep marine biosphere habitats. These patterns are consistent with the sources of sediment in the River Tyne being predominantly terrestrial in origin. The results point to microbial communities in estuarine and marine sediments being structured by bi-directional currents, terrestrial run-off and industrial effluent as vectors of passive dispersal and immigration.

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Section: Discussioncontrasting

confidence: 79%

Distribution of thermophilic endospores in a temperate estuary indicate that dispersal history structures sediment microbial communities

Bell

Blake

Sherry

et al. 2018

Environmental Microbiology

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“…The latter explanation is supported by viable counts which show that pasteurized incubations exhibit almost the same numbers as non-pasteurized incubations (data not shown). Further, the numbers of endospores increase from about 1% of the total cell counts in Janssand surface sediments to 10% at 5 mbsf indicating an increasing number of bacteria in this resting stage with depth (Fichtel et al, 2008). At site JS-A, pasteurized and non-pasteurized incubations result in similar numbers at 0.5 and 18 mbsf as for site JS-B.…”

Section: Imprint Of Present Environmental Conditionsmentioning

confidence: 56%

Imprint of past and present environmental conditions on microbiology and biogeochemistry of coastal Quaternary sediments

et al. 2011

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Abstract. To date, North Sea tidal-flat sediments have been intensively studied down to a depth of 5 m below seafloor (mbsf). However, little is known about the biogeochemistry, microbial abundance, and activity of sulfate reducers as well as methanogens in deeper layers. In this study, two 20 m-long cores were retrieved from the tidal-flat area of Spiekeroog Island, NW Germany. The drill sites were selected with a close distance of 900 m allowing to compare two depositional settings: first, a paleo-channel filled with Holocene sediments and second, a mainly Pleistocene sedimentary succession. Analyzing these cores, we wanted to test to which degree the paleo-environmental imprint is superimposed by present processes.In general, the numbers of bacterial 16S rRNA genes are one to two orders of magnitude higher than those of Archaea. The abundances of key genes for sulfate reduction and methanogenesis (dsrA and mcrA) correspond to the sulfate and methane profiles. A co-variance of these key genes at sulfate-methane interfaces and enhanced ex situ AOM rates suggest that anaerobic oxidation of methane may occur in these layers. Microbial and biogeochemical profiles are vertically stretched relative to 5 m-deep cores from shallower sediments in the same study area, but still appear compressed compared to deep sea sediments. Our interdisciplinary analysis shows that the microbial abundances and metabolic rates are elevated in the Holocene compared to Pleistocene sediments. However, this is mainly due to present environmental Correspondence to: M. Beck (m.beck@icbm.de) conditions such as pore water flow and organic matter availability. The paleo-environmental imprint is still visible but superimposed by these processes.

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“…Based on measurements of the endospore‐specific compound dipicolinic acid (DPA) endospores are estimated to be as abundant as vegetative cells in subsurface sediments (Langerhuus et al ., ; Lomstein et al ., ; Braun et al . ; Fichtel et al ., ) and their presence is also documented by cultivation dependent enumeration (Volpi et al ., ; de Rezende et al ., ; Hubert et al ., ; Köpke et al ., ).…”

Section: Introductionmentioning

confidence: 83%

“…However, endospores also occur in high abundances in marine sediments, although the seabed is a homogenous and stable environment (Hoehler and Jørgensen, 2013). Based on measurements of the endospore-specific compound dipicolinic acid (DPA) endospores are estimated to be as abundant as vegetative cells in subsurface sediments Lomstein et al, 2012;Braun et al 2017;Fichtel et al, 2008) and their presence is also documented by cultivation dependent enumeration (Volpi et al, 2017;de Rezende et al, 2013;Hubert et al, 2009;Köpke et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Contrasting community composition of endospores and vegetative Firmicutes in a marine sediment suggests both endogenous and exogenous sources of endospore accumulation

Cupit

Lomstein

Kjeldsen

2018

Environ Microbiol Rep

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Bacterial endospores are highly abundant in marine sediments, but their taxonomic identity and ecology is largely unknown. We selectively extracted DNA from endospores and vegetative cells and sequenced 16S rRNA genes to characterize the composition of the endospore and vegetative Firmicutes communities in the sediment and water column of Aarhus Bay (Denmark). The endospore community in the sediment was dominated by the families Bacillaceae, Lachnospiraceae, Clostridiaceae and Ruminoccocaceae. These families were also represented in the vegetative community in the sediment and the endospore community in the water column. OTUs of high relative abundance in the endospore community were also represented in the vegetative Firmicutes community. Other OTUs were exclusively found in the endospore communities. This suggests that endospores accumulate in marine sediments due to passive deposition from the water column and sporulation of vegetative cells in the sediment. Some OTUs were detected in the endospore community of the water column and the vegetative community the sediment indicating that endospores deposited from the water column may germinate upon burial/deposition in the sediment. We provide novel insight into the composition of endospore communities in marine sediments and highlight their role in microbial dispersal and as a seed bank in subsurface sediments.

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High Variations in Endospore Numbers within Tidal Flat Sediments Revealed by Quantification of Dipicolinic Acid

Cited by 16 publications

References 32 publications

Distribution of thermophilic endospores in a temperate estuary indicate that dispersal history structures sediment microbial communities

Distribution of thermophilic endospores in a temperate estuary indicate that dispersal history structures sediment microbial communities

Imprint of past and present environmental conditions on microbiology and biogeochemistry of coastal Quaternary sediments

Contrasting community composition of endospores and vegetative Firmicutes in a marine sediment suggests both endogenous and exogenous sources of endospore accumulation

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