In this contribution, four automatic adjustment algorithms for leakage carrier cancellation in radio frequency identification (RFID) readers are compared: full search, gradient search, fast and direct I/Q algorithms. Further, we propose two enhanced adjustment procedures. First, we analytically calculate the performance of the fast adjustment algorithm in the presence of noise and derive its theoretical bias. We compare the theoretical results with the numerical results from accompanying simulations. Further, we evaluate the performance of these algorithms based on real-world measurements acquired with our RFID testbed. Finally, we propose and discuss the merits of two enhanced adjustment procedures based on the fast adjustment algorithm. The fast adjustment procedure with bipolar probing signals achieves the isolation gain of the (much slower) gradient search algorithm at the expense of a mean penalty of 0.48 dB. We observe that the fast adjustment aided gradient algorithm requires 72% less steps than the gradient search algorithm in our measurements.