High turnover and rescue effect of XRCC1 in response to heavy charged particle radiation

Liu, Wenjing; Wu, Ruqun; Guo, Junbo; Shen, C.; Zhao, Jing; Mao, Guangbo; Mou, Hongjin; Zhang, Lei; Du, Guanghua

doi:10.1016/j.bpj.2022.03.011

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…Considering the recruitment time of XRCC1, we found that it is dependent mainly on the deposited energy for both α-particles and protons, with a minimum value of 34 s following irradiation by 1000 α-particles. The measured recruitment times measured in this study are compatible with the ones reported by Liu et al with higher LET charged particles [61].…”

Section: Discussionsupporting

confidence: 92%

Recruitment Kinetics of XRCC1 and RNF8 Following MeV Proton and α-Particle Micro-Irradiation

Muggiolu

Torfeh

Simón

et al. 2023

Biology

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Time-lapse fluorescence imaging coupled to micro-irradiation devices provides information on the kinetics of DNA repair protein accumulation, from a few seconds to several minutes after irradiation. Charged-particle microbeams are valuable tools for such studies since they provide a way to selectively irradiate micrometric areas within a cell nucleus, control the dose and the micro-dosimetric quantities by means of advanced detection systems and Monte Carlo simulations and monitor the early cell response by means of beamline microscopy. We used the charged-particle microbeam installed at the AIFIRA facility to perform micro-irradiation experiments and measure the recruitment kinetics of two proteins involved in DNA signaling and repair pathways following exposure to protons and α-particles. We developed and validated image acquisition and processing methods to enable a systematic study of the recruitment kinetics of GFP-XRCC1 and GFP-RNF8. We show that XRCC1 is recruited to DNA damage sites a few seconds after irradiation as a function of the total deposited energy and quite independently of the particle LET. RNF8 is recruited to DNA damage sites a few minutes after irradiation and its recruitment kinetics depends on the particle LET.

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Section: Discussionsupporting

confidence: 92%

Recruitment Kinetics of XRCC1 and RNF8 Following MeV Proton and α-Particle Micro-Irradiation

Muggiolu

Torfeh

Simón

et al. 2023

Biology

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Multi-omics landscape and molecular basis of radiation tolerance in a tardigrade

Li,

Ge,

Liu

et al. 2024

Science

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Tardigrades are captivating organisms known for their resilience in extreme environments, including ultra-high-dose radiation, but the underlying mechanisms of this resilience remain largely unknown. Using genome, transcriptome, and proteome analysis of Hypsibius henanensis sp. nov. , we explored the molecular basis contributing to radiotolerance in this organism. A putatively horizontally transferred gene, DOPA dioxygenase 1 ( DODA1 ), responds to radiation and confers radiotolerance by synthesizing betalains—a type of plant pigment with free radical–scavenging properties. A tardigrade-specific radiation-induced disordered protein, TRID1, facilitates DNA damage repair through a mechanism involving phase separation. Two mitochondrial respiratory chain complex assembly proteins, BCS1 and NDUFB8, accumulate to accelerate nicotinamide adenine dinucleotide (NAD + ) regeneration for poly(adenosine diphosphate–ribosyl)ation (PARylation) and subsequent poly(adenosine diphosphate–ribose) polymerase 1 (PARP1)–mediated DNA damage repair. These three observations expand our understanding of mechanisms of tardigrade radiotolerance.

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High turnover and rescue effect of XRCC1 in response to heavy charged particle radiation

Cited by 2 publications

References 37 publications

Recruitment Kinetics of XRCC1 and RNF8 Following MeV Proton and α-Particle Micro-Irradiation

Recruitment Kinetics of XRCC1 and RNF8 Following MeV Proton and α-Particle Micro-Irradiation

Multi-omics landscape and molecular basis of radiation tolerance in a tardigrade

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