High tolerance to apoptotic stimuli induced by serum depletion and ceramide in side-population cells: High expression of CD55 as a novel character for side-population

Xu, Jing-Xian; Morii, Eiichi; Nakamichi, Naoto; Ikeda, Jun‐ichiro; Kimura, Hayato; Aozasa, Katsuyuki

doi:10.1016/j.yexcr.2007.03.006

Cited by 37 publications

(32 citation statements)

References 32 publications

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“…This broad spectrum is also observed in MCF7 breast cancer cell line (22). Very recently, we found that CD55-high population in the MCF7 cells is resistant to apoptotic stimuli and forms colonies in vitro more efficiently than CD55-low population (22). CD55 expression level is higher in SP cells than in non-SP of MCF7 cells.…”

supporting

confidence: 59%

“…Staining of HT29 colon cancer cell line with anti-CD55 antibody showed a broad spectrum of expression levels among cancer cells (19). This broad spectrum is also observed in MCF7 breast cancer cell line (22). Very recently, we found that CD55-high population in the MCF7 cells is resistant to apoptotic stimuli and forms colonies in vitro more efficiently than CD55-low population (22).…”

mentioning

confidence: 60%

“…Center). MCF7 cells were sorted as CD55-high population and CD55-low population as described previously (22).…”

Section: Methodsmentioning

confidence: 99%

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Prognostic Significance of CD55 Expression in Breast Cancer

Ikeda¹,

Morii²,

Liu³

et al. 2008

Clinical Cancer Research

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Purpose: Our recent study revealed that CD55-high population in breast cancer cell line was resistant to apoptosis and formed colonies in vitro more efficiently than CD55-low population. The present study was conducted to examine whether CD55-high population in breast cancer cell line possesses higher tumorigenic potential in vivo and presence of CD55-high cells in breast cancer affects clinicopathologic behavior of patients. Experimental Design: CD55-high and CD55-low population was sorted from breast cancer cell line, injected into immunodeficient mice, and the resultant tumor volume was measured. CD55 expression was immunohistochemically examined in clinical samples from 74 cases with breast cancers, and cases with >1% of tumor cells showing high level of CD55 expression were categorized as CD55 high. Results: The xenotransplanted tumor volume derived from CD55-high population was significantly larger than that from CD55-low population. Fifty (67.6%) of 74 cases of breast cancer were CD55-high. A significant correlation was observed between CD55-high character and relapse rate (P < 0.001). Univariate analysis showed that tumor size (P = 0.005) and CD55 expression (P = 0.005) were unfavorable prognostic factors. Multivariate analysis revealed that the tumor size (P = 0.013) and CD55 expression (P = 0.011) were independent prognostic factors. Conclusions: CD55 play an important role in tumorigenesis of breast cancer, and presence of small population of cells with strong CD55 expression would be sufficient to predict poor prognosis of patients.

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confidence: 59%

mentioning

confidence: 60%

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Prognostic Significance of CD55 Expression in Breast Cancer

Ikeda¹,

Morii²,

Liu³

et al. 2008

Clinical Cancer Research

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“…The possible toxicity of Hoechst 33342 may create bias by selectively injuring non-SP cells thus bias any comparative tests of cancer SP and Non-SP cells. 80,81 However, recent work by Chiba et al, demonstrated that the difference of Hoechst 33342 toxicity on cancer SP and Non-SP cells is marginal and may not explain the failure of Non-SP cells to sustain proliferation in vitro or to form tumors in vivo. 42 To reduce the potential bias by Hoechst 33342, alternative low toxicity dyes, like Cycle Violet (also known as Vybrant Violet), could be used for SP staining.…”

Section: Isolation and Enrichment Of Cancer Stem Cellsmentioning

confidence: 99%

Cancer stem cells: Models, mechanisms and implications for improved treatment

Zhou

Zhang

2008

Cell Cycle

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Cancer stem cell research has drawn a lot of attention recently. The importance of cancer stem cell research lies in the possibility of providing new approaches for improved understanding of cancer biology and cancer treatment. However, cancer stem cell research is still at its infancy. In this review, we will discuss the concept of cancer stem cells and the commonly used approaches for isolating or enriching cancer stem cells. We then review the common features of cancer stem cells including resistance to cancer drugs and stresses such as hypoxia and radiation. We also summarize signaling pathways which might be preferentially important for cancer stem cells. Finally, we will propose a new Yin-Yang model of cancer stem cells and discuss the possibility of developing new drugs that target cancer stem cells for improved treatment of cancer.

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“…These rare cell populations possessed an increased ability to form tumors when they were injected into etoposide-treated NOD/ SCID mice; as few as 100 CD44 + /cd24 -human breast cancer cells could recapitulate the human mammary tumors, whereas the injection of 10,000 cells with other phenotypes failed to give rise to tumors (2). Since then, several techniques have been established to isolate or enrich for tumorigenic breast cancer stem cells (BCSCs), including side-population (SP) separation and mammosphere culture (6,7). In breast cancer, the mammosphere culture system has been widely used to identify and enrich for putative stem cells using breast cancer cell lines (8).…”

Section: Introductionmentioning

confidence: 99%