High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

Méndez-Pérez, Roberto; García-López, Rodrigo; Bautista-López, J. Santiago Bautista-López; Álvarez-González, Carlos Alfonso; Peña-Marín, Emyr Saúl; Baltierra-Trejo, Eduardo; Adams-Schroeder, Randy; Domínguez-Rodríguez, V. I.; Melgar-Valdes, Carolina E.; Martínez‐García, Rafael; Moya, Andrés; Gómez-Cruz, Rodolfo

doi:10.3856/vol48-issue3-fulltext-2419

Cited by 10 publications

(6 citation statements)

References 107 publications

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“…The composition of Fusobacteria and Proteobacteria in E. stellifer is similar to that found in other marine fish, such as Silurus and Carassium 24 , and to results reported for freshwater garpike 32 . A few studies have shown Fusobacteria to be the dominant members of the gut microbiota of freshwater fish 33 , 34 .…”

Section: Discussionsupporting

confidence: 86%

Gut microbiota analysis of Blenniidae fishes including an algae-eating fish and clear boundary formation among isolated Vibrio strains

Yoshida

Tanabe

Akiyoshi

et al. 2022

Sci Rep

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Some marine fishes are algae-feeding, and the microorganisms in their digestive tracts produce carbohydrate hydrolyzing enzymes such as agarose and fucosidase, which are potentially interesting resource for new functional enzymes. The purpose of this study was to establish a method for identifying and utilizing characteristic bacteria from the intestines of two algae-eating fish species: Andamia tetradactylus, which exclusively eats algae on the rock surface, and stellar rockskipper Entomacrodus stellifer, which feeds on both algae and invertebrates. We tested the species composition of the intestinal bacterial flora and found that Proteobacteria were commonly found both in species as in the common gut communities of marine fish, whereas Spirochaetes and Tenericutes occupied the flora of A. tetradactylus. We then performed anaerobic and aerobic cultures and isolated 34 and 44 strains including 48 strains belonged to Vibrio species from A. tetradactylus and E. stellifer. We observed that some Vibrio strains formed a clear boundary to avoid contacting other strains of bacteria. Whole-genome sequencing of such two Vibrio alginolyticus strains revealed two cyclic chromosomes commonly found in the genome of Vibrio species, and some unique genes encoding alginate lyase, chitinases, and type I-F CRISPR-associated endoribonuclease for the first time in Vibrio alginolyticus.

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Section: Discussionsupporting

confidence: 86%

Gut microbiota analysis of Blenniidae fishes including an algae-eating fish and clear boundary formation among isolated Vibrio strains

Yoshida

Tanabe

Akiyoshi

et al. 2022

Sci Rep

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“…Gut microbial alpha diversity did not differ between the sexes in G. japonicus, similar to the result reported for a wide range of vertebrates including fish [61], amphibians [62], birds [57], and mammals [63]. However, sexual differences in gut microbial diversity do exist in many animals, also including fish [64], birds [65], and mammals [66]. Taken together, available data show that dietary and/or sexual correlates of host gut microbial alpha diversity are species-or taxonspecific.…”

Section: Discussionsupporting

confidence: 85%

Dietary and Sexual Correlates of Gut Microbiota in the Japa-Nese Gecko, Gekko japonicus (Schlegel, 1836)

Jiang¹,

Dai²,

Wang³

et al. 2023

Preprint

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Numerous studies have demonstrated that multiple intrinsic and extrinsic factors shape the structure and composition of gut microbiota in a host. The disorder of gut microbiota may trigger various host diseases. Here, we collected fecal samples from wild-caught Japanese geckos (Gekko japonicus) and captive conspecifics fed with mealworms (mealworm-fed geckos) and fruit flies (fly-fed geckos), aiming to examine dietary and sexual correlates of gut microbiota. We used the 16S rRNA gene sequencing technology to determine the composition of gut microbiota. The dominant phyla with a mean relative abundance higher than 10% were Verrucomicrobiota, Bacteroidota and Firmicutes. Gut microbial community richness was higher in mealworm-fed geckos than in fly-fed and wild geckos, and community diversity was higher in mealworm-fed geckos than in wild geckos. Neither alpha nor beta diversity of gut microbiota differed among wild, mealworm-fed and fly-fed geckos. The beta rather than alpha diversity of gut microbiota was sex-dependent. Based on the relative abundance of gut bacteria and its gene functions, we concluded that gut microbiota contributed more significantly to the host’s metabolic and immune functions. Higher diversity of gut microbiota in mealworm-fed geckos could result from higher chitin contents of insects of the order Coleoptera. This study not only provides basic information about the gut microbiota of G. japonicus, but also shows that gut microbiota correlates with dietary habit and sex in the species.

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“…Gut microbial alpha diversity did not differ between the sexes in G. japonicus , similar to the results reported for a wide range of vertebrates, including fish [ 62 ], amphibians [ 63 ], birds [ 58 ], and mammals [ 64 ]. However, sexual differences in gut microbial diversity do exist in many animals, including fish [ 65 ], birds [ 66 ], and mammals [ 67 ]. Taken together, the available data show that dietary and/or sexual correlates of host gut microbial alpha diversity are species- or taxon-specific.…”

Section: Discussionmentioning

confidence: 99%

Dietary and Sexual Correlates of Gut Microbiota in the Japanese Gecko, Gekko japonicus (Schlegel, 1836)

Jiang

Dai

Wang

et al. 2023

Animals

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Numerous studies have demonstrated that multiple intrinsic and extrinsic factors shape the structure and composition of gut microbiota in a host. The disorder of the gut microbiota may trigger various host diseases. Here, we collected fecal samples from wild-caught Japanese geckos (Gekko japonicus) and captive conspecifics fed with mealworms (mealworm-fed geckos) and fruit flies (fly-fed geckos), aiming to examine the dietary and sexual correlates of the gut microbiota. We used 16S rRNA gene sequencing technology to determine the composition of the gut microbiota. The dominant phyla with a mean relative abundance higher than 10% were Verrucomicrobiota, Bacteroidota, and Firmicutes. Gut microbial community richness and diversity were higher in mealworm-fed geckos than in wild geckos. Neither community evenness nor beta diversity of gut microbiota differed among wild, mealworm-fed, and fly-fed geckos. The beta rather than alpha diversity of gut microbiota was sex dependent. Based on the relative abundance of gut bacteria and their gene functions, we concluded that gut microbiota contributed more significantly to the host’s metabolic and immune functions. A higher diversity of gut microbiota in mealworm-fed geckos could result from higher chitin content in insects of the order Coleoptera. This study not only provides basic information about the gut microbiota of G. japonicus but also shows that gut microbiota correlates with dietary habits and sex in the species.

show abstract

High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

Cited by 10 publications

References 107 publications

Gut microbiota analysis of Blenniidae fishes including an algae-eating fish and clear boundary formation among isolated Vibrio strains

Gut microbiota analysis of Blenniidae fishes including an algae-eating fish and clear boundary formation among isolated Vibrio strains

Dietary and Sexual Correlates of Gut Microbiota in the Japa-Nese Gecko, Gekko japonicus (Schlegel, 1836)

Dietary and Sexual Correlates of Gut Microbiota in the Japanese Gecko, Gekko japonicus (Schlegel, 1836)

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