2022
DOI: 10.1016/j.cell.2022.09.038
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High-throughput sequencing of single neuron projections reveals spatial organization in the olfactory cortex

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Cited by 61 publications
(57 citation statements)
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References 132 publications
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“…Because many barcode molecules can be sequenced in parallel, barcoding-based neuroanatomical approaches can determine the long-range projections of tens of thousands of neurons in single animals. These barcoding-based techniques have been applied to various brain regions (Kebschull et al ., 2016; Han et al, 2018; Chen et al ., 2019; Gergues et al, 2020; Klingler et al, 2021; Mathis et al, 2021; Munoz-Castaneda et al, 2021; Sun et al ., 2021; Chen et al, 2022b) and have validated and extended findings by previous studies using conventional neuroanatomical techniques.…”
Section: Introductionsupporting
confidence: 71%
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“…Because many barcode molecules can be sequenced in parallel, barcoding-based neuroanatomical approaches can determine the long-range projections of tens of thousands of neurons in single animals. These barcoding-based techniques have been applied to various brain regions (Kebschull et al ., 2016; Han et al, 2018; Chen et al ., 2019; Gergues et al, 2020; Klingler et al, 2021; Mathis et al, 2021; Munoz-Castaneda et al, 2021; Sun et al ., 2021; Chen et al, 2022b) and have validated and extended findings by previous studies using conventional neuroanatomical techniques.…”
Section: Introductionsupporting
confidence: 71%
“…Such space-based rules in connectivity are common in vertebrate brains (e.g. retinotopy, cortical column) and many may exist independent of transcriptomic type-based differences in connectivity (Chen et al ., 2022b); specific spatial patterns of connectivity loss are common in many neurodegenerative diseases. By combining in situ sequencing with barcoded rabies tracing, we expand in situ sequencing-based barcoded neuroanatomical approaches to include anterograde projection mapping (BARseq), multiplexed retrograde tracing, and multiplexed transsynaptic tracing.…”
Section: Discussionmentioning
confidence: 99%
“…While in wild type mice unbiased machine learning approaches identify at least 5 distinct OR expression patterns, in the triple Nfi cKO mice these patterns become intermixed for all but zone 1 ORs. With recent observations arguing that individual mitral cells, the second order neurons in the olfactory circuit, have patterned projections in the brain [49], non-random OR expression in the MOE may contribute to putative hardwired components of odor perception and valance [50].…”
Section: Discussionmentioning
confidence: 99%
“…MAPseq and BARseq have been repeatedly validated using multiple methods in a wide range of brain areas (Zeisler et al 2023; Y. Chen et al 2022; Hausmann et al 2022; Tsoi et al 2022; Webb et al 2022; Klingler et al 2021; Mathis et al 2021; Muñoz-Castañeda et al 2021; Sun et al 2021; Gergues et al 2020; Huang et al 2020; X. Chen et al 2019; Han et al 2018; Kebschull et al 2016).…”
Section: Introductionmentioning
confidence: 99%