High throughput sequencing of 18S rRNA and its gene to characterize a Prorocentrum shikokuense (Dinophyceae) bloom

Zhang, Yaqun; Lin, Xin; Li, Tangcheng; Li, Hengde; Lin, Lingxiao; Luo, Hao; Li, Ling; Ji, Nanjing; Lin, Senjie

doi:10.1016/j.hal.2020.101809

Cited by 11 publications

(5 citation statements)

References 62 publications

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“…These dinoflagellate homologs share conserved residues with bacteria PR, including retinal pocket, proton donor and receptor, and light tuning (Lin et al 2010;Shi et al 2015). Shi et al (2015) found that PR in the dinoflagellate, Prorocentrum donghaisense (recently renamed as P. shikokuense; see Zhang et al 2020), exhibited a strong diel rhythm in transcript abundance: high Edited by Chengchao Chen.…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression and cell membrane localization of dinoflagellate opsins (rhodopsin proteins) in mammalian cells

Shi

Lin

2020

Mar Life Sci Technol

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Rhodopsins are now found in all domains of life, and are classified into two large groups: type II, found in animals and type I found in microbes including Bacteria, Archaea, and Eukarya. While type II rhodopsin functions in many photodependent signaling processes including vision, type I among others contains rhodopsins that function as a light-driven proton pump to convert light into ATP as in proteobacteria (named proteorhodopsin). Proteorhodopsin homologs have been documented in dinoflagellates, but their subcellular localizations and functions are still poorly understood. Even though sequence analyses suggest that it is a membrane protein, experimental evidence that dinoflagellate rhodopsins are localized on the plasma membrane or endomembranes is still lacking. As no robust dinoflagellate gene transformation tool was available, we used HEK 293T cells to construct a mammalian expression system for two dinoflagellate rhodopsin genes. The success of expressing these genes in the system shows that this mammalian cell type is suitable for expressing dinoflagellate genes. Immunofluorescence of the expressed protein locates these dinoflagellate rhodopsins on the cell membrane. This result indicates that the protein codons and membrane targeting signal of the dinoflagellate genes are compatible with the mammalian cells, and the proteins' subcellular localization is consistent with proton pump rhodopsins.

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Section: Introductionmentioning

confidence: 99%

Heterologous expression and cell membrane localization of dinoflagellate opsins (rhodopsin proteins) in mammalian cells

Shi

Lin

2020

Mar Life Sci Technol

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“…Metabarcoding and metatranscriptome profiling with next-generation sequencing have proven to be effective ways to profile community composition and metabolic landscape ( 19 , 20 ) and to detect major shifts in metabolic activities and resource utilization in the course of phytoplankton succession ( 21 , 22 ). Metatranscriptomic studies have successfully tracked cellular processes associated with life history transitions, nutrient acquisition, defense, and energy production during dinoflagellate blooms by community gene expression analyses ( 23 – 30 ). Previous data also suggested that sexual reproduction (meiosis) could contribute to bloom formation for dinoflagellate blooms ( 25 , 31 ).…”

Section: Introductionmentioning

confidence: 99%

In Situ Molecular Ecological Analyses Illuminate Distinct Factors Regulating Formation and Demise of a Harmful Dinoflagellate Bloom

et al. 2023

Microbiol Spectr

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“…Since 2001, blooms of P. donghaiense have occurred annually in the similar area of the ECS (Lu D. et al, 2005;Lu et al, 2014). Because of the severity of economic and ecological impacts of these blooms, P. donghaiense has been extensively investigated from many facets during the last two decades, including its tolerance to extreme or changing salinity, temperature, and light intensity (Chen et al, 2005;Xu et al, 2010;Boyd et al, 2013;Hu et al, 2014Hu et al, , 2016, nutrient requirements (Huang et al, 2005;Hong et al, 2009;Ou et al, 2010;Hu et al, 2012Hu et al, , 2016Zhang et al, 2012;Jing et al, 2017), method development for species detection (Wang et al, 2007;Chen et al, 2011Chen et al, , 2013, effects on and interactions with other organisms (Wang et al, 2003(Wang et al, , 2020Yan et al, 2007;Lin et al, 2014;Li et al, 2018;Shi et al, 2018), effects on resource utilization (Chai et al, 2020), and blooming dynamics and oceanographic mechanisms (Cai et al, 2002;Zhu et al, 2009;Li et al, 2010;Dai et al, 2013Dai et al, , 2014Zhang et al, 2020). All these studies helped us to better understand the biology and ecology of this species, but it is still highly controversial regarding where the initial "seeding population" came from for the annual blooms.…”

Section: Introductionmentioning

confidence: 99%

The Notorious Harmful Algal Blooms-Forming Dinoflagellate Prorocentrum donghaiense Produces Sexual Resting Cysts, Which Widely Distribute Along the Coastal Marine Sediment of China

Liu

Deng

2022

Front. Mar. Sci.

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The armored dinoflagellate Prorocentrum donghaiense distributes globally and has been forming large scale and dense ecosystem disruptive algal blooms (EDABs) in the East China Sea (ECS) almost every year since the 1990s and often in other coastal waters of the world. It has long been a mystery, however, about how these blooms were seeded or where the initiating population came from. In this work, we provide a more feasible and universal seeding mechanism, formation of resting cysts. Using light microscopy, we confirmed sexual reproduction according to the observations of mating cells in pairs, planozygotes having two similar flagella, darkened and thick-walled resting cysts with smooth surface, and germination processes of resting cyst. Using morpho-molecular detection, we confirmed P. donghaiense resting cyst in the field, including the positive detections of polymerase chain reaction (PCR) using species-specific primers and then the fluorescence in situ hybridization (FISH) using species-specific probes, and further confirmation via single-cell sequencing for the individual FISH-detected cysts. Furthermore, the distribution and abundance of P. donghaiense cysts along the coast of China Seas were mapped using an approach combining real-time PCR (qPCR) and FISH, with the qPCR quantification taking into account the doubled copy number of LSU rRNA gene in resting cysts. Resting cysts of this species were found to widely distribute in the Yellow Sea (YS), ECS, and South China Sea (SCS), with a relatively low abundance at most sampling sites, but to be absent in the eight samples from the Bohai Sea (BS). Resting cyst production confirmed with evidences from both laboratory cultures and field sediments and the extensive distribution of cysts in the China Seas, as the first case in planktonic species of Prorocentrum, not only filled up a knowledge gap about the life history of P. donghaiense but also provided a possible mechanistic facility to seed the annual blooms in the ECS and the global distribution of the species.

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High throughput sequencing of 18S rRNA and its gene to characterize a Prorocentrum shikokuense (Dinophyceae) bloom

Cited by 11 publications

References 62 publications

Heterologous expression and cell membrane localization of dinoflagellate opsins (rhodopsin proteins) in mammalian cells

Heterologous expression and cell membrane localization of dinoflagellate opsins (rhodopsin proteins) in mammalian cells

In Situ Molecular Ecological Analyses Illuminate Distinct Factors Regulating Formation and Demise of a Harmful Dinoflagellate Bloom

The Notorious Harmful Algal Blooms-Forming Dinoflagellate Prorocentrum donghaiense Produces Sexual Resting Cysts, Which Widely Distribute Along the Coastal Marine Sediment of China

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